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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar questions and answers(New questions) [Re: van hatton]
#25646047 - 11/29/18 05:19 AM (5 years, 2 months ago) |
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If I use 10:8 nute for speed would you advice using a higher nutes before transfer or doesnt it matter and reasons please.
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Agar questions and answers(New questions) [Re: Edmunter]
#25646054 - 11/29/18 05:26 AM (5 years, 2 months ago) |
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Uh.
Use a low ratio 2% is standard I use 1.5-1.75%
I like to keep my stuff constant so the mycelium doesn't make any strange moves on me and end up looking weird on me.
Stick with the ratio that works for u.
Reason I wouldn't do higher nutes specifically is because rizo mycelium doesn't happen very often on higher nutes plates. The nice thing about rizo is it's super easy to tell that from contams. So I shoot for rizo.
Does that make sense?
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar questions and answers(New questions) [Re: van hatton]
#25646325 - 11/29/18 09:46 AM (5 years, 2 months ago) |
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One of the TCs was saying lower nute make nicer looking plate but its best to use higher nute plates to inocutate with. Im just trying get confirmation here.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Agar questions and answers(New questions) [Re: Edmunter]
#25646336 - 11/29/18 09:54 AM (5 years, 2 months ago) |
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I run 2% nutes across the board.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar questions and answers(New questions) [Re: Mycolorado]
#25698443 - 12/23/18 02:34 PM (5 years, 1 month ago) |
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So if you wanted to start a new master how long should you leave a plate b4 using it. I took a dime size from a plate the other day and cut just inside the outer growth. My thinking is that this part is clean and even if the plate has something funky on it I should be ok.
What's your thinking/experience on this subject?
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar questions and answers(New questions) [Re: Edmunter]
#25699528 - 12/24/18 07:17 AM (5 years, 1 month ago) |
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Looks good so far
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar questions and answers(New questions) [Re: Edmunter]
#25727030 - 01/06/19 11:35 AM (5 years, 24 days ago) |
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Ive started some new videos to see how my technique is working.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar questions and answers(New questions) [Re: Edmunter]
#25727032 - 01/06/19 11:38 AM (5 years, 24 days ago) |
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar questions and answers(New questions) [Re: Edmunter]
#25727037 - 01/06/19 11:39 AM (5 years, 24 days ago) |
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar questions and answers(New questions) [Re: Edmunter]
#25737460 - 01/11/19 04:52 AM (5 years, 19 days ago) |
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So ive had a bit of a eureka moment that what im trying to do is grow a culture that is 100% clean on the culture itself regardless of whether the plate has a few spots. In these photos I am quite happy they are clean and wondered what options I have? Can you transfer out even when its really small to get rid of side contaminations if you are still learning your sterile technique? Can you comment on these?
 This looks clean
 This looks clean
 This looks clean
 From a pin that appeared on a plate 1 transfer away from use
Edited by Edmunter (01/11/19 05:02 AM)
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar questions and answers(New questions) [Re: Edmunter]
#25737476 - 01/11/19 05:03 AM (5 years, 19 days ago) |
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From PE transfer

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