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OfflineEdmunter
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Agar questions and answers
    #23857076 - 11/22/16 11:44 AM (7 years, 2 months ago)

So I have some stuff going on with agar.  Id like to understand more about whats going on.

This P.Mex plate is DFA 8:10 did something strange on transfer.  It fluffed up b4 begining to row.  It happened to all 3 plates.  WTF is going on here then?

v

This P. Sandose has been producing spots and not growing like id like it to since the first transfer.  Do these have a history of this kinda growth



These PE plates both have gaps where it looks like it either hasnt liked the agar there or it's genetics?



This AA+ has produced a pin before the culture has reached the edge,  Im thinking I want those genetics as shes a white cracker bursting with life.  Transferring soon




India O plates are looking good.  Lots of nice sectors very clear



Thai Plate is looking good and getting closer to Isolate



Finally this Eringii clone from a shop bought mushroom is doing well and will be grown out to inoculate rye seed soon.



If you can help please add some comments.


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Offlineenlightenment
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Re: Agar questions and answers [Re: Edmunter]
    #23857165 - 11/22/16 12:16 PM (7 years, 2 months ago)

If the agar is very nutritious the transfered pieces tend to fluff sometimes IME. You should not worry about that. I had this with different species that got nice growth later.
I have no experience with DF agar. Usually I use MEA.
The PE plate looks strange. Observe it and update this thread in 2 days.


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InvisiblebodhisattaMDiscordReddit
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Re: Agar questions and answers [Re: enlightenment]
    #23857169 - 11/22/16 12:17 PM (7 years, 2 months ago)

:whathesaid:


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InvisibleComebackKid
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Re: Agar questions and answers [Re: bodhisatta]
    #23857549 - 11/22/16 02:38 PM (7 years, 2 months ago)

My last batch of PDA plates all look like those fluff balls you have there. Too much nutrients


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OfflineEdmunter
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Re: Agar questions and answers [Re: Edmunter]
    #23859935 - 11/23/16 08:57 AM (7 years, 2 months ago)

I have 2 aa+ plates with pins.  After cloning  Is it ok to use these for LI and get decent results?


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InvisibleMunchauzen
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Re: Agar questions and answers [Re: Edmunter]
    #23860028 - 11/23/16 09:24 AM (7 years, 2 months ago)

I would not spawn pins to bulk. Fruits are a vector for cubensis.


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OfflineEdmunter
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Re: Agar questions and answers [Re: Munchauzen]
    #23860044 - 11/23/16 09:27 AM (7 years, 2 months ago)

So clone the pin and LI the plate so I get a  MS grow?  I kinda want some for xmas so im in a hurry.


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Offlinewtfcrazymofo
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Re: Agar questions and answers [Re: Edmunter]
    #23860466 - 11/23/16 12:27 PM (7 years, 2 months ago)

Eat that pin merry x mas.


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineEdmunter
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Re: Agar questions and answers [Re: Edmunter]
    #23862882 - 11/24/16 08:06 AM (7 years, 2 months ago)

These PE plate certainly dont like those parts of the agar.  Ok on 1 plate but to have 2 do the same suggests something else.  The other sides on both have nice sectoring going on.  I have take 3 transfers from 1 plate and hope for better all round genetics.




There she blows!  I was too late on one plate but the other was ok. so I made a LI and clones the Pin.





On a side note, can you use Cat food as a medium?


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OfflineEdmunter
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Re: Agar questions and answers [Re: Edmunter]
    #23877196 - 11/29/16 03:53 AM (7 years, 2 months ago)

So the P.mex after the initial fluffing has grown out nice and cleanly and round.  Im thinking of using this for a master jar now.  Here are the latest photos



The lids a bit clouded due to old age but it looks fine to me.


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InvisibleComebackKid
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Re: Agar questions and answers [Re: Edmunter]
    #23877269 - 11/29/16 05:12 AM (7 years, 2 months ago)

I litterally just did the same thing a few days ago. My plate was super fluffy but perfectly round like yours. I cut it into 36 pieces and nocd up 6 jars with it.
The growth in jars is suprisingly the most rhizo I have ever had yet.




--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineEdmunter
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Re: Agar questions and answers [Re: ComebackKid]
    #23877722 - 11/29/16 09:49 AM (7 years, 2 months ago)

One of the PE plates kinda colonised the patch.  What could of caused this?



I transferred away from the last plate but the new plate looks a bit sweaty.

The sectoring the on left of this plate is really clear and looking good.

Gunna transfer once more.


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OfflineEdmunter
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Re: Agar questions and answers [Re: Edmunter]
    #23922686 - 12/13/16 01:27 PM (7 years, 1 month ago)

4th Transfer

PE



Made a LI with another similar which is going for it, so happy days.

Doubt I will be getting any Penis for christmas though which is a shame as I was really looking forward to some festive fun.

I heard somebody say they had never had a blob when they had straight cased which is interesting.

Where would you take from to get close to isolate?  I take it if I take one tiny wedge from the single stains that will isolate?  I know its not neccesarry but Id like a few isolates as ive never gone that far.



7 oclock looks quite uniform


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OfflineEdmunter
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Re: Agar questions and answers(New questions) [Re: Edmunter]
    #23983112 - 01/05/17 04:41 AM (7 years, 25 days ago)

So with my shinny silver pants im cooking the A again


So questions of the day
Are these aa+ pins?



What are these, blobs?


Is this a contamination?



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OfflineEdmunter
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Re: Agar questions and answers(New questions) [Re: Edmunter]
    #23986062 - 01/06/17 09:08 AM (7 years, 24 days ago)

Quote:

Edmunter said:
So with my shinny silver pants im cooking the A again


So questions of the day
Are these aa+ pins?



What are these, blobs?


Is this a contamination?





The answer I got from another forum was

Yes
Knots
Yes

I m not sure if it is a contamination but there dont seem to be too many experts on this subject.



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OfflinePsilocyBen17
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Re: Agar questions and answers(New questions) [Re: Edmunter]
    #23986073 - 01/06/17 09:17 AM (7 years, 24 days ago)

:threadmonitor:


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OfflineEdmunter
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Re: Agar questions and answers(New questions) [Re: Edmunter]
    #23986086 - 01/06/17 09:25 AM (7 years, 24 days ago)

My next agar question is why does this culture do this?  It has done it since the first transfer away from spores and now I am at the 5th.





So when they start growing they leave an area like this.  Has anyone had this b4 and what causes it?  It has done it with every plate involved from the original plate.



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InvisibleMycolorado
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Re: Agar questions and answers(New questions) [Re: Edmunter]
    #23986127 - 01/06/17 09:54 AM (7 years, 24 days ago)

The dark stuff looks like sediment from the dog food...you're coloring your agar, correct?  If so, as the color fades, the sediment becomes more apparent...possibly.


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OfflinePsilocyBen17
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Re: Agar questions and answers(New questions) [Re: Mycolorado]
    #23986162 - 01/06/17 10:09 AM (7 years, 24 days ago)

that looks like a nice sector


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OfflineEdmunter
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Re: Agar questions and answers(New questions) [Re: Edmunter]
    #23986206 - 01/06/17 10:31 AM (7 years, 24 days ago)

Quote:

Edmunter said:
My next agar question is why does this culture do this?  It has done it since the first transfer away from spores and now I am at the 5th.





So when they start growing they leave an area like this.  Has anyone had this b4 and what causes it?  It has done it with every plate involved from the original plate.







Why does it grow round on itself and miss out that section?  I was thinking of using this to make a LI.  What do you think?


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