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OfflineOldYamaha
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Liquid Culture, Liquidize Grain spawn to substrate
    #23854653 - 11/21/16 03:33 PM (7 years, 2 months ago)

I have tried to make liquid culture from grain spawn, and the method is working with several attempts. Has anyone tried to do this same thing, adding sterilized water to jars of grain spawn and blending the grain/mycelium up and then putting the blended spawn into a hardwood pellet tec?
The liquid culture from grain spawn to more grain has produced very good results, why wouldn't this same process work with lime pasteurized sawdust?
I have 14 quart jars ready to go to substrate and thought, can this be done? Has anyone tried to do this?


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Offlineprimus
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Re: Liquid Culture, Liquidize Grain spawn to substrate [Re: OldYamaha]
    #23854920 - 11/21/16 04:47 PM (7 years, 2 months ago)

why go through the process of making liquid culture from grain spawn only to inoculate grain with it? cant you just do a grain to grain transfer?

if you have colonized grain, the most sterile and fastest way to colonize substrate would be to add the grain to the substrate. in my experince grain to sawdust colonizes very quickly. are you trying to speed up the process even further? by turning your grain spawn into liquid you are adding another possible contamination point.

curious... how do you blend the grain mixture and keep it sterile?


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OfflineOldYamaha
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Re: Liquid Culture, Liquidize Grain spawn to substrate [Re: primus]
    #23855727 - 11/21/16 09:24 PM (7 years, 2 months ago)

That's just it, the sawdust pellets are just poured into my 55 gallon drum, with 4#s of wheat bran, 1# gypsum, 72 grams of Hydrated lime, 10#s of spawn, and 6-7 gallons of water. Everything is cold, I have been using this method for over 1 year now and no contamination issues. However if the grain spawn was liquefied there would then be many more inoculation points hence a faster (hopefully?) consolidation time. SEE THIS https://www.shroomery.org/forums/showflat.php/Number/19402655#19402655
At least that was my thought. If you can speed up grain to grain using a liquid culture made this same way, SEE THIS TEC https://www.shroomery.org/forums/showflat.php/Number/10648149/fpart/1/vc/1
which I have used to speed up my grain to grain inoculation, then why can't this be used for the grain to substrate step?

<<curious... how do you blend the grain mixture and keep it sterile?>>
With a standard food processor. At this point in the process its the last step before bagging. Keeping it as sterile as possible is no longer the major consideration. But yes you want to be clean and sterile as possible, but the hydrated lime will inhibit the nasties until the mycelium has swallowed them. And if this would then speed up the consolidation of the mycelium it would be ?more better?:grin:


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Edited by OldYamaha (11/21/16 09:34 PM)


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InvisibleMarty Mycfly
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Re: Liquid Culture, Liquidize Grain spawn to substrate [Re: OldYamaha]
    #23855880 - 11/21/16 10:34 PM (7 years, 2 months ago)

It seems difficult to do on a large scale like that, and risky.
However if you want to try it you can use an Oster blender blade attachment, they fit onto a regular mouth quart jar perfect, I use one to make LI sometimes.



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OfflineQuadman
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Re: Liquid Culture, Liquidize Grain spawn to substrate [Re: Marty Mycfly]
    #23855927 - 11/21/16 10:59 PM (7 years, 2 months ago)

If grain works for you then LI should.


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Offlineanthiawe
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Re: Liquid Culture, Liquidize Grain spawn to substrate [Re: Quadman]
    #23856223 - 11/22/16 03:28 AM (7 years, 2 months ago)



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