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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 22 days
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Anything I can salvage?
#23853863 - 11/21/16 10:36 AM (7 years, 2 months ago) |
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Okay I can pretty tell that most of these petris are done for.
Petri C there seems to be something I can take, highlighted red circle, but it looks like it's surrounded by mold
Petri E looks cleanish, the upper right red circle is where mycelium is developing, with no mold surrounding it
Petri F looks the cleanist, with the bottom of the petri with mold spores and mycelium, while upper right, red circle, looks like mycelium developing a distance from the contamination
Anything I can salvage or are they mostly done for ??
My idea was to take a couple of wedges from Petri E and F and transfer those onto agar plates
Also is mycelium meant to look so invisible and whispy on agar?
I can barely detect it sometimes unless I flip the petri a certain way and the light gives away a greyish-whispy hue on the surface

TOP LANE

MIDDLE LANE

BOTTOM LANE
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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ComebackKid
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Re: Anything I can salvage? [Re: Rosen_Rot]
#23853915 - 11/21/16 10:53 AM (7 years, 2 months ago) |
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All you can do is try man. It's definitely going to take a lot of work to clean these up. I like to tease a little mycelium off the agar surface with the tip of my scalpel when it's contam central. Rather than slicing a big piece of agar. Not sure if this is reccomend but it seems to work for me so far. 
Yeas sometimes before the myc thickens the outer ring will be almost invisable
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Rosen_Rot
Learning



Registered: 12/06/14
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On closer inspection of the plates I have noticed a lot, of what seems like, strandy mycelium and now I am not sure if I am cultivating mold or its the actual mycelium growing in a mixture of tomentose and rhizomorphic mycelium
What confuses me is that there is always a greyish spot/invisble area and then from that area white strands of mycelium is coming out. I am not sure if the invisible spot is the place of inoculation and if mycelium is developing from it and the left over liquid from the spore syringe OR if the actual invisible ring is the mycelium itself and it's being engulfed from what looks like mold.
Ugh this kind of stuff you get with experience and I am not sure what to look out for
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
Edited by Rosen_Rot (11/21/16 11:12 AM)
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ComebackKid
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Re: Anything I can salvage? [Re: Rosen_Rot]
#23853986 - 11/21/16 11:16 AM (7 years, 2 months ago) |
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Yeah I just checked out your pics. I'm not liking any of them tbh but maybe get someone else's opinion before throwing them away. Personally I would start over
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Mycolorado
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Registered: 07/23/16
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Re: Anything I can salvage? [Re: Rosen_Rot]
#23853993 - 11/21/16 11:18 AM (7 years, 2 months ago) |
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Hey Rot,
All those plates have some degree of bacterial contam....they all came from the same syringe, correct? I would transfer from the top 2 plates as they appear to have the best growth. You might try backing down the nutrient content of your medium to help promote more rhizomorphic growth, which in turn, will outrun the bacteria in its search for nutes. I have good luck with 3%. What's the recipe you're using?
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ComebackKid
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Re: Anything I can salvage? [Re: Mycolorado]
#23854009 - 11/21/16 11:21 AM (7 years, 2 months ago) |
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What's your explanation for the hollow centers? And the specks of random myc surrounding the outer edge?
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Mycolorado
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Registered: 07/23/16
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Hey Comeback,
My guess would be the contams (on the hollow centers)...I've seen it on a couple plates I've grown from syringe and that are showing bacteria...I just keep transferring from the outside until it cleans up. You might be right on starting over though as sometimes they're just a lost cause. Either way, it's good practice. The satelites could be due to poor technique or it's myc from spores deposited from the carrying water being rolled around on the plate, maybe...?
Edited by Mycolorado (11/21/16 11:28 AM)
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 22 days
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Re: Anything I can salvage? [Re: Mycolorado]
#23854024 - 11/21/16 11:27 AM (7 years, 2 months ago) |
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Yes they are all from the same spore syringe. The recipe I am using is 10gram cat food, 10 gram agar and 500ml water. I could knock it down to 4g cat food and 10gram agar
I just threw away the first 2 plates in the trash  You were talking about plates A and D right??

Fuck and double fuck
What would your next suggestion be?
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Quote:
ComebackKid said: What's your explanation for the hollow centers? And the specks of random myc surrounding the outer edge?
Mostly due to bacteria IME.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Anything I can salvage? [Re: Teemo 6T3]
#23854039 - 11/21/16 11:33 AM (7 years, 2 months ago) |
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Btw how long did you sterilise the cat food for?
Because according to Pasty they require more time than normal recipes like MEA for sterilisation.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Mycolorado
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Registered: 07/23/16
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Re: Anything I can salvage? [Re: Rosen_Rot]
#23854041 - 11/21/16 11:34 AM (7 years, 2 months ago) |
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Yeah...they looked like they had the most organized areas of growth that would allow for a decent transfer. Hmm...that's a 2% nute concentration, so in theory, it should promote rhizo as opposed to tomentose mycelium. Try a different nute source...I get really different growth characteristics on MEA v. DFA. If you can't get MEA and don't have dog food, try the same recipe with oatmeal instead...grind it up and use in place of the cat food. I've had good luck with it as well. Like I said in the other thread...ditch the cat food.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
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Loc: ked in the trunk of a car
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Re: Anything I can salvage? [Re: Mycolorado]
#23854043 - 11/21/16 11:35 AM (7 years, 2 months ago) |
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Yeah I never thought maybe the spores were spread with an Inoc loop? Cool good to know, I would have immediately assumed mold. Rosen rot, I apologize if you toseed those because of me. I hope they are salvageable
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 22 days
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Re: Anything I can salvage? [Re: Mycolorado]
#23854177 - 11/21/16 12:29 PM (7 years, 2 months ago) |
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Would wheetabix suffice? They have Thaimine B1, fibre and protein but it's made of wheat not oats, not sure if that makes a world of difference. As far as my knowledge goes Thaimine encourages mycelial growth. If not wheetabix would quaker oats be good oats to use in the recipe?
I have some other plates made from the same CFA recipe. I'll wait for growth from those plates, make new plates with the new recipe and transfer from there
BTW I am really appreciating your responses, this is helping out a lot in tackling the vast ocean of agar 
ComeBackKid; it's alright. I threw them away cause I genuinely thought they were bad and moldy as well. I should have trusted my judgement to be completely honest, I should have known to recognize mycelium when I see it. Ah well, learning curve, pretty slow one too haha
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
Edited by Rosen_Rot (11/21/16 12:31 PM)
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Anything I can salvage? [Re: Rosen_Rot]
#23854264 - 11/21/16 01:05 PM (7 years, 2 months ago) |
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This is the easiest agar tek ever .
You can find the ingredients in any grocery store.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
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Re: Anything I can salvage? [Re: Rosen_Rot]
#23854400 - 11/21/16 02:01 PM (7 years, 2 months ago) |
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Quote:
Rosen_Rot said: Would wheetabix suffice? They have Thaimine B1, fibre and protein but it's made of wheat not oats, not sure if that makes a world of difference. As far as my knowledge goes Thaimine encourages mycelial growth. If not wheetabix would quaker oats be good oats to use in the recipe?
I use quaker oats. I would try both and see how they compare. Not sure where you're located but if there is a homebrew store in your area I recommend you pick up some powdered light malt extract for a couple bucks a pound which should last quite a while...MEA is a great all around medium for a variety of species. I tend to make smaller batches (200ml - makes 7-8 plates)and work with fewer plates so it's not a total plate-fest. More importantly, for me at least, is that it allows for experimentation with different nutrients and nutrient %s and not ending up with 20 plates of something that doesn't work great...
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 22 days
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Re: Anything I can salvage? [Re: Mycolorado]
#23855078 - 11/21/16 05:47 PM (7 years, 2 months ago) |
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Yes I am beginning to see the error and my learning cycle, I made entire batches of the one recipe when just experimenting, thing is, so many teks tell you to make spares that I got paranoid. If anything I have noticed so far in CFA is that growth is pretty slow but then again I haven't done much experimentation. I think switching the recipe to a grain substance would be better.
I'll have a look around the local brewery, see if they have any MEA.
Quacker Oats and Wheetabix are also very easy to acquire
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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