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InvisiblexPTxHIPPIe
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Registered: 06/13/16
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Quick agar question
    #23852946 - 11/20/16 11:12 PM (7 years, 2 months ago)

So I have been working with agar for a few months now and have just recently ran into some major contam problems with every transfer I do. I'm using pasty plates and I am under the assumption it is my potatoe flakes are old but my untapped plates aren't growing any thing. So my next assumption is I'm just transfering some contams and I feel like maybe they will go away if I catch the mycellium before the contams get worse. I snatch a piece of clean tissue every time but there is just so much contam.


So I guess my question is if you read all of that. Do I just need to keep making transfers to get rid of the contain?


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Invisiblevan hattonFacebookDiscord
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Re: Quick agar question [Re: xPTxHIPPIe]
    #23852972 - 11/20/16 11:35 PM (7 years, 2 months ago)

If you UN inoculated plates are fine then that's not the problem Eh.

I assume your transferring mycelium that has mold intertwined in it.

pic would help


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisiblexPTxHIPPIe
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Registered: 06/13/16
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Re: Quick agar question [Re: van hatton]
    #23852991 - 11/20/16 11:47 PM (7 years, 2 months ago)



Yeah I think your right my last plate probably grew out to much but I thought taking the transfer from the oppsite end of the plate would be safe.


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Invisiblevan hattonFacebookDiscord
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Re: Quick agar question [Re: xPTxHIPPIe]
    #23853006 - 11/20/16 11:59 PM (7 years, 2 months ago)

Is that green on your plate my phone sucks hard to tell.

When your problems started did you transfer from a plate with green on it?


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisibleBoogieman47
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Re: Quick agar question [Re: van hatton]
    #23853018 - 11/21/16 12:04 AM (7 years, 2 months ago)

Open a plate that is really gone and let us see in the plate ... i had some issues with trich in the myc its a fucked situation and may not be fixable... if its bacteria then its your sterile tek do you atleast wipe your knjfe handle with iso?


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Offlineafflictis
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Re: Quick agar question [Re: Boogieman47]
    #23853022 - 11/21/16 12:06 AM (7 years, 2 months ago)

Hard to tell from the pic but that plate looks infected


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InvisibleBoogieman47
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Re: Quick agar question [Re: Boogieman47]
    #23853023 - 11/21/16 12:06 AM (7 years, 2 months ago)

here are some bacterial and aspergillus plates


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Invisiblevan hattonFacebookDiscord
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Re: Quick agar question [Re: afflictis]
    #23853027 - 11/21/16 12:09 AM (7 years, 2 months ago)

My thought is that he had a plate contam on him. It sportulated and while he was doing his transfer spores got knocked around possibly to the new agar.

He said he let one grow out so the contain is possibly intertwined in the myc.

Must of figured it was clean and transfer a bunch :shrug:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Offlineafflictis
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Re: Quick agar question [Re: Boogieman47]
    #23853028 - 11/21/16 12:09 AM (7 years, 2 months ago)

Quote:

noob47 said:
here are some bacterial and aspergillus plates


on that third pic that rope looking growth isn't mycelium?


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InvisibleBoogieman47
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Re: Quick agar question [Re: afflictis]
    #23853030 - 11/21/16 12:11 AM (7 years, 2 months ago)

Ya but you notice all the black shit? That was a pe clone that got super fucked


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InvisiblexPTxHIPPIe
Inspired
I'm a teapot


Registered: 06/13/16
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Re: Quick agar question [Re: Boogieman47]
    #23853040 - 11/21/16 12:15 AM (7 years, 2 months ago)

Wow yup noob47 is right looks just alike all of my others. I plan to start from scratch anyways.



Edited by xPTxHIPPIe (11/21/16 12:17 AM)


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Offlineafflictis
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Re: Quick agar question [Re: Boogieman47]
    #23853054 - 11/21/16 12:23 AM (7 years, 2 months ago)

Quote:

noob47 said:
Ya but you notice all the black shit? That was a pe clone that got super fucked



Haha now I do


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InvisibleBoogieman47
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Re: Quick agar question [Re: xPTxHIPPIe]
    #23853055 - 11/21/16 12:23 AM (7 years, 2 months ago)

Thats just some bacteria honestly the way it looks is either youre not waiting long enough after the spray or you need to wipe your handle off really good try holding the plate upside down in your sab ... you can clean that up man


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Offlineafflictis
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Re: Quick agar question [Re: xPTxHIPPIe]
    #23853059 - 11/21/16 12:24 AM (7 years, 2 months ago)

Quote:

xPTxHIPPIe said:
Wow yup noob47 is right looks just alike all of my others. I plan to start from scratch anyways.




yea that's all kinds of fucked


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Invisiblevan hattonFacebookDiscord
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Re: Quick agar question [Re: afflictis]
    #23853069 - 11/21/16 12:29 AM (7 years, 2 months ago)

9-11 o clock small transfers and you'll get that clean good luck.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Invisiblemorty422
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Registered: 07/06/16
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Re: Quick agar question [Re: van hatton]
    #23853102 - 11/21/16 12:49 AM (7 years, 2 months ago)

Quote:

van hatton said:
9-11 o clock small transfers and you'll get that clean good luck.




I agree. Not too far gone yet. I've isolated away from much worse.

Be serious about your sterile tek and you can get away from that.

Are you switching your agar type up at all? I've found a change of agar nutrients can help in isolating away from nasties.

Sometimes the change in medium can give mycelium a chance to outrun the contamination a bit.

I'd like to say that it's a bit of luck that's needed as well...

Keep trying brother-you'll grab it soon. I've had some tricky ones as well from some recent syringes I got...

:cheers:


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InvisiblexPTxHIPPIe
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Registered: 06/13/16
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Re: Quick agar question [Re: morty422]
    #23853269 - 11/21/16 04:19 AM (7 years, 2 months ago)

Lol thanks for all the input. The only thing iv changed was I made 2 batch one with karo and one without. Using the pasty plate recipe and forgot to add that so I made a second batch. Lol I have more fun messing with the agar than my tubs.


Maybe I need to wait a little longer. I most soapy water on the sab almost like I would a tub or cakes.

I recall letting my myc touch some green shit and thought I could just transfer from the other side of it


Edited by xPTxHIPPIe (11/21/16 04:22 AM)


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