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PieEyed-Messiah
MANDINGO



Registered: 11/24/15
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Storage/long term
#23852475 - 11/20/16 07:10 PM (7 years, 2 months ago) |
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Ok soo i think i may have bitten off more than i can chew...i recently traded for a lamanarflow hood and alot of edible gormet wedges about 18 to be exact and was wondering how to go about storeing these i would eventuallly like to fruit and collect spore prints /untill i can manage that monumental task how would i keep the myc vaible? My plan was to make at least 2 dishes from each wedge(do i really have to change agar nutrients every 30 days ?) Should i store them in fridge after colonization?would a slant last longer?
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Quadman
Challenged


Registered: 04/23/16
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If agar is poured thick and dish is sealed with parafilm they will keep months in the fridge. Slants will keep longer, I use 50 ml tubes with 25 ml of agar for slants. The man problem is for the culture to dry out.Some are storing wedges on sterile water for extended time with no refrigeration.
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Gr0wer
always improving



Registered: 09/16/03
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Re: Storage/long term [Re: Quadman] 1
#23853652 - 11/21/16 09:17 AM (7 years, 2 months ago) |
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I use 1/4 pint jars with thick agar and toothpicks, works well. I also store backup cultures in sterile water.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: Storage/long term [Re: Gr0wer]
#23853752 - 11/21/16 09:55 AM (7 years, 2 months ago) |
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Toothpicks are an amazing addition to any mycology toolbox. Nice video
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filyep
Dr.



Registered: 07/12/16
Posts: 131
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Re: Storage/long term [Re: Ferather]
#23855243 - 11/21/16 06:45 PM (7 years, 2 months ago) |
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So what exactly are the toothpicks for?
Additional food for the mycelium?
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Gr0wer
always improving



Registered: 09/16/03
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Re: Storage/long term [Re: filyep]
#23855254 - 11/21/16 06:48 PM (7 years, 2 months ago) |
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Yes food, they also offer a easy to grab chunk.
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Shu
Vote for Humanity



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Re: Storage/long term [Re: filyep]
#23857741 - 11/22/16 03:28 PM (7 years, 2 months ago) |
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According to GGMM the wood seems to provide longevity where agar alone failed. I just finished resuscitation and passage of my entire collection that was put on antibiotic MEA slants with toothpicks in October 2014 and every one survived. Prior to putting them in the refrigerator I opened the caps 1/4 turn and wraped with 3 windings of parafilm and put them in a ziplock bag. To resuscitate I just pulled out the picks and dropped them on MEA.
P.S I bend the picks in half before wedging them in the tubes then rotate the tubes while cooling so the don't get entirely submerged.
Edited by Shu (11/22/16 03:32 PM)
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Ferather
Mycological



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Re: Storage/long term [Re: Shu]
#23857760 - 11/22/16 03:31 PM (7 years, 2 months ago) |
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Thats the exact same findings I have achieved. I am temped to see what pink does on wood at lower temps.
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Shu
Vote for Humanity



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Re: Storage/long term [Re: Shu]
#23859637 - 11/23/16 06:27 AM (7 years, 2 months ago) |
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Ferather
Mycological



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Re: Storage/long term [Re: Shu]
#23859659 - 11/23/16 06:43 AM (7 years, 2 months ago) |
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Nice hover method, your own idea?
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PieEyed-Messiah
MANDINGO



Registered: 11/24/15
Posts: 611
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Re: Storage/long term [Re: Ferather]
#23881221 - 11/30/16 10:53 AM (7 years, 1 month ago) |
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Would this woulrk for cubes aswell as gormet?
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keifnnugs
Master fucker
Registered: 11/27/15
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Has anyone stored cultures in LC.?
Was wondering this since venders will send syringes out and mine have lasted over a year at room temp
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PieEyed-Messiah
MANDINGO



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Re: Storage/long term [Re: keifnnugs]
#23882518 - 11/30/16 06:01 PM (7 years, 1 month ago) |
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lc is a nutrient souloution which tends to go bad after a while ime should i store emmediatly after making in refridgerator?
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Quadman
Challenged


Registered: 04/23/16
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You can store cubes on agar slants. Only wood lovers will benefit from wood in the slant.LC aren't ideal storage. If you go with liquid just use sterile water and store wedges in water filled tube.
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Ferather
Mycological



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Posts: 6,325
Loc: United Kingdom
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Re: Storage/long term [Re: Quadman]
#23883677 - 12/01/16 05:16 AM (7 years, 1 month ago) |
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Cubes will transfer to wood and from wood, not ideal. However if you watch Gr0wer's video you will see he uses them for other reasons also. One of them which I think is a great idea is to hold down the agar in place. Removal is also easier, limited agar contact.
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keifnnugs
Master fucker
Registered: 11/27/15
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Re: Storage/long term [Re: Ferather]
#23885669 - 12/01/16 08:02 PM (7 years, 1 month ago) |
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I was thinking to guarantee clean storage in water you could do no pour agar in 1/2 pint with an LC lid.. Add a wedge to let the dish colonize then inject x ml sterile water through the LC lid, shake it like crazy then draw the water back up and store in sterile centrifuge tube, test tubes or whatever else you want to use..
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keifnnugs
Master fucker
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Re: Storage/long term [Re: keifnnugs]
#23888463 - 12/02/16 06:18 PM (7 years, 1 month ago) |
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So is this how most people store their cultures?
I recently got a bunch of cultures that I want to make sure I don't lose.. My plan is to get the LC syringes on agar.. After its clean I was going to drop a bunch of small wedges into waster like the tek suggests as well as make thick agar jars like Gr0wer for the fridge..
Is it safe to leave the cultures in water at room temp for years? Or is it smart to take each culture to agar ever 6 months to a year then back to storage?
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Gr0wer
always improving



Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX
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Re: Storage/long term [Re: keifnnugs]
#23889072 - 12/02/16 09:29 PM (7 years, 1 month ago) |
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From what ive read the sterile water puts the culture in suspended animation of sorts. Especiallly with a layer of mineral oil on top of the water. So no refrigeration is needed for years. I ran into a few of my water cultures to contaminate so now i use them as a last resort backup and run the small half pint jars with about an inch of agar and toothpicks as my go to masters. Also, always have multiple masters especially if your a production grower like me. I have 3 jars of each and 3 water cultures of each of my main strains.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Storage/long term [Re: Gr0wer]
#23889780 - 12/03/16 07:00 AM (7 years, 1 month ago) |
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The oil will also help prevent oxygen dissolving into the water. No 02, no nutrients or energy = stall, dormancy.
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PieEyed-Messiah
MANDINGO



Registered: 11/24/15
Posts: 611
Last seen: 2 days, 21 hours
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Re: Storage/long term [Re: Gr0wer]
#23889957 - 12/03/16 08:56 AM (7 years, 1 month ago) |
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Quote:
Gr0wer said: From what ive read the sterile water puts the culture in suspended animation of sorts. Especiallly with a layer of mineral oil on top of the water. So no refrigeration is needed for years. I ran into a few of my water cultures to contaminate so now i use them as a last resort backup and run the small half pint jars with about an inch of agar and toothpicks as my go to masters. Also, always have multiple masters especially if your a production grower like me. I have 3 jars of each and 3 water cultures of each of my main strains.
shit im trying to be a production grower like you but youve some mighty shoes to fill my friend
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Mattisfat
Learning


Registered: 01/20/16
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keifnnugs
Master fucker
Registered: 11/27/15
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Re: Storage/long term [Re: Gr0wer]
#23890394 - 12/03/16 11:53 AM (7 years, 1 month ago) |
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Quote:
Gr0wer said: From what ive read the sterile water puts the culture in suspended animation of sorts. Especiallly with a layer of mineral oil on top of the water. So no refrigeration is needed for years. I ran into a few of my water cultures to contaminate so now i use them as a last resort backup and run the small half pint jars with about an inch of agar and toothpicks as my go to masters. Also, always have multiple masters especially if your a production grower like me. I have 3 jars of each and 3 water cultures of each of my main strains.
Thanks for the response.. I was hoping to store everything in water in small tubes but if you save the thick agar jars are better I'll go with that and have the water as back up..
I need to find some small jars that I can fit in a lunchbag in my fridge.. Would baby food jars work? Storing 10 half pints will take up to much space in the fridge.. Thinking of something smaller until I get my mini fridge
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Storage/long term [Re: keifnnugs]
#23890407 - 12/03/16 11:57 AM (7 years, 1 month ago) |
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Anything that can contain fluids and can be made to be sterile, in working order.
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JesseCruz
Stranger
Registered: 02/29/16
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Re: Storage/long term [Re: Ferather]
#23894425 - 12/04/16 04:35 PM (7 years, 1 month ago) |
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How about anaerobic storage in sterile distilled water?
Dram vials of mycelium water may keep for several years at room temperature. I'm refining a syringe-based method based on this tek.
The literature describing this method focuses on yeasts and pathogens, but it seems reliable for gourmet mushrooms too.
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keifnnugs
Master fucker
Registered: 11/27/15
Posts: 335
Last seen: 26 days, 7 hours
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Re: Storage/long term [Re: JesseCruz]
#23894667 - 12/04/16 06:01 PM (7 years, 1 month ago) |
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Would you guys recommend storing in vacutainers? Or would it be better using screw top test tubes? Would you pc the vacutainers with water in them? Or would it be better to LC them in a jar and use sterile water from a syringe to fill them when doing the agar to vacutainer transfer?
If anyone can throw a like up of good test tubes that would be great
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Quadman
Challenged


Registered: 04/23/16
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Loc: IL
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Re: Storage/long term [Re: keifnnugs]
#23894827 - 12/04/16 06:46 PM (7 years, 1 month ago) |
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Watch the video in the beginning of this thread.
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keifnnugs
Master fucker
Registered: 11/27/15
Posts: 335
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Re: Storage/long term [Re: Quadman]
#23894982 - 12/04/16 07:38 PM (7 years, 1 month ago) |
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Where in the video does he mention the size of the test tubes? Where in the video does he mention vacutainers?
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Gr0wer
always improving



Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX
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Re: Storage/long term [Re: keifnnugs]
#23895102 - 12/04/16 08:09 PM (7 years, 1 month ago) |
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The source, size and details of the tubes is in the videos comments.
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keifnnugs
Master fucker
Registered: 11/27/15
Posts: 335
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Re: Storage/long term [Re: Gr0wer]
#23896190 - 12/05/16 08:52 AM (7 years, 1 month ago) |
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Ahh lol I wanted the video like 20 times.. When watching it in the player it doesn't show comments unless I open in YouTube.. Thanks Gr0wer
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PieEyed-Messiah
MANDINGO



Registered: 11/24/15
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Re: Storage/long term [Re: keifnnugs]
#23896224 - 12/05/16 09:08 AM (7 years, 1 month ago) |
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I have adopted this method and have all my species under steril h2o ive yet to do any transfers (grower have you noticed contamination also being sort of in suspended cultivation due to lac of o2 and nutrients?)or will the contams just take over completly?
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keifnnugs
Master fucker
Registered: 11/27/15
Posts: 335
Last seen: 26 days, 7 hours
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Found this great write up that shows how to use various containers including vacutainers..
https://www.shroomery.org/forums/showflat.php/Number/12968316/fpart/1/vc/1
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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Re: Storage/long term [Re: keifnnugs]
#23917321 - 12/11/16 06:22 PM (7 years, 1 month ago) |
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My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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keifnnugs
Master fucker
Registered: 11/27/15
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If anyone is interested 15ml centrifuge tube are like $15 for 100 Or if you want some fat boys you can get the 50ml tubes..
I'll be getting both. I'll use the 15ml for water storage and 50ml for the thick agar like in Gr0wers video
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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Re: Storage/long term [Re: keifnnugs]
#23917727 - 12/11/16 08:03 PM (7 years, 1 month ago) |
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I wouldn't know from personal experience, but the Care and Handling of Cultures write-up by Penn state says to never put cultures in a fridge that doesn't have fresh air circulation (i.e., anaerobic storage). Is it possible that in an aerobic fridge with proper humidity a culture would last longer? Culture banks obviously use other freezing and cooling techniques to store cultures for decades, so is anaerobic refrigeration the 'best case scenario' since dehydration is the main problem for the home cultivator?
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My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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