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OfflineJapaneseson
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I searched high and low...What does this look like?
    #23852447 - 11/20/16 06:55 PM (7 years, 2 months ago)

Will post pics


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OfflineJapaneseson
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23852504 - 11/20/16 07:20 PM (7 years, 2 months ago)



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OfflineJapaneseson
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23852510 - 11/20/16 07:22 PM (7 years, 2 months ago)



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OfflineJapaneseson
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23852517 - 11/20/16 07:25 PM (7 years, 2 months ago)

Looks waterlogged, soggy, with some ex-puff balls that went kinda green to me...Man, I dug and dug and dug for some answers here.

Bottom line...What is the diagnosis here?


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OfflineJapaneseson
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23854330 - 11/21/16 01:26 PM (7 years, 2 months ago)

Help a brother?

Background: So I spawned bags, added to HP that was pasteurized. Waited 10 days then introduced to FC...Here is where I think I went wrong...I misted in a hybrid tub. Pretty sure the humidity dipped below range, but the sub had yellow pooling that I hoped would absorb. I thought bringing down Temps after full colonization would protect the cakes. Then I got some fuzzy looking balls which I thought were knots. That's the 3 spots that look greenish.


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OfflineTira
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Registered: 11/20/10
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23854417 - 11/21/16 02:09 PM (7 years, 2 months ago)

That just looks bruised and bacterial.


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OfflineJapaneseson
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23854459 - 11/21/16 02:25 PM (7 years, 2 months ago)

So that contam could have happened anywhere between spawn-to-sub and somewhere after introducing to FC?

I went with the theory the spawn/sub intro was pretty safe. The spawn/sub didn't even see ambient air particulates until after 10 days of incubation.


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OfflineTira
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Registered: 11/20/10
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23854507 - 11/21/16 02:49 PM (7 years, 2 months ago)

The spawn is exposed the momentit came out from the bags and was mixed with the substrate.  It doesn't matter (greatly) if you kept it closed during incubation or not, what matters is if the substrate was pasteurized properly and kept in ideal conditions.  Keeping it too wet (misting more than the substrate's needs) may cause bacterial growth.

But the most important question is, was your spawn clean? Did you do grains, how long did you PC, did you inoculate with spore syringe, LC or agar?


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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OfflineJapaneseson
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Re: I searched high and low...What does this look like? [Re: Tira]
    #23854660 - 11/21/16 03:35 PM (7 years, 2 months ago)

Thanks bro. Bought the bags (rye) and sub from a reputable source. The LC could be culprit. A failure on the part of the source could have contributed. Obviously I could have contributed.

Looks like I'm going to start tightly controlling everything...Agar to fruit


Edited by Japaneseson (11/21/16 04:09 PM)


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OfflineJapaneseson
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23854680 - 11/21/16 03:39 PM (7 years, 2 months ago)

The smells were right. Temps right. Sub not too wet.


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OfflineJapaneseson
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23854804 - 11/21/16 04:11 PM (7 years, 2 months ago)

I got the shitake end of the stick!:thumbdown:


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
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Re: I searched high and low...What does this look like? [Re: Japaneseson]
    #23854891 - 11/21/16 04:37 PM (7 years, 2 months ago)

No problem at all. Starting agar is a very good idea.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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OfflineJapaneseson
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Re: I searched high and low...What does this look like? [Re: Tira]
    #23892176 - 12/03/16 09:35 PM (7 years, 1 month ago)

Update: my shittake mycelium was healthy...And this post is my 10th, so now I can see shit I couldn't before!


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