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acidninja
student



Registered: 09/08/16
Posts: 1,693
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Last seen: 2 months, 10 days
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Over 2 months and not even half colonized yet
#23851643 - 11/20/16 02:16 PM (7 years, 2 months ago) |
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when people said that growing mushrooms requires "patience" I didn't know it would go on for almost 2 or so months, by now I'm starting to wounder if these jars are even still alive . . . I've been tripping on Libs since I went out with Joel and started picking them in September and at the same time I inoculated the jars. Anyway here's the story; So I inoculated 4 jars in my sterilized bathroom with cube spores and left them in my room on a shelf with a little desk light for about a month and they were doing fine, the temp was around 70f in the day and around 60f at night and the humidity was around 80-85%, but when October came the house started to get colder and I could see the growth was slowing so I decided to stick the jars in my boiler room, I checked on them after an hour and the humidity had plunged to around 20% but the temperature was well over (and at a constant) 80f, that's when weird shit started to happen, some jars stopped completely whilst others spread aggressively and then stopped and then started again, in the end (tonight) I've decided to finally put them in my cupboard above my cooker and now there are a constant 80-85% humidity and around 75-85f temperature, but what I really want to know is why are they taking so long?
Anyway here are the details:
Jar 1: 375ml Jam/Mason Jar - Inoculated on the 8th of September - Psilocybe Cubensis Golden Teacher - Myc growth: Started off aggressive then completely stopped
Jar 2: 375ml Jam/Mason Jar - Inoculated on the 5th of September - Psilocybe Cubensis Cambodian - Myc Growth: Started off slow and then stopped completely
Jar 3: 375ml Jam/Mason Jar - Inoculated on the 8th Of September - Psilocybe Cubensis koh samui Myc Growth: Started Off Fast and then stopped completely
jar 4: 375ml Jam/Mason jar - Inoculated on the 5th of September - Psilocybe Cubensis Cambodian - Myc Growth: Started off fast and is now growing at a slow but constant rate
Can anyone help me out? I will also post pics if necessary.
Thanks AJ
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Over 2 months and not even half colonized yet [Re: acidninja]
#23851715 - 11/20/16 02:47 PM (7 years, 2 months ago) |
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I am curious about how you sterilized your bathroom? If your jars stopped all of a sudden they are done, as in bad toss em. Healthy jars from MS should be none in no more than 3 weeks, and that's being liberal, not to mention MS is a crapshoot, can be done but also has a chance to be contaminated.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Over 2 months and not even half colonized yet [Re: acidninja]
#23851723 - 11/20/16 02:49 PM (7 years, 2 months ago) |
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jars crap out from bad water content or contam.
they are taking to long because have been fucking with them too much you are the 'hamburger grill' looking incubation set up guy right? but chances are the prep wasnt done correctly.
why are you even concerned with humidity while colonizing? even during fruiting, humidity is nothing you really need to worry about unless you live in a arid climate.
you cant sterilize a bathroom. too many shit particles build a SAB for 5$
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acidninja
student



Registered: 09/08/16
Posts: 1,693
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Re: Over 2 months and not even half colonized yet [Re: Marty Mycfly]
#23851738 - 11/20/16 02:55 PM (7 years, 2 months ago) |
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so toss and next time dont fuck with em, got it, oh and btw just a quick side note as well, the spores I were using were kinda . . . well . . . 11 years old, sitting in a syringe in Joel's fridge, he gave em to me as a gift
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Over 2 months and not even half colonized yet [Re: acidninja]
#23851747 - 11/20/16 02:59 PM (7 years, 2 months ago) |
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if the spores germinated, then the age is inconsequential in regards to colonizing speed.
and check your jar prep. dont inoc in a bathroom. take away more then 'dont fuck with them'
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Re: Over 2 months and not even half colonized yet [Re: mushboy]
#23851758 - 11/20/16 03:02 PM (7 years, 2 months ago) |
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Post the pics you mentioned. Were the jars ever shaken? I presume you have filters, and know they are OK.
I have also seen a dramatic drop in growth as the cold weather has come in. I think this is normal & expected, but if you read many threads here you will see people saying low temps like 60 or 65F are perfectly fine and just a little slower -many of whom I guess never grew at the temps they mention. They are just reiterating what they heard before, or else do not want to back down after saying the vague "room temperature is fine" line and then find out that persons room is far lower than they would have actually thought.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Over 2 months and not even half colonized yet [Re: mushboy]
#23851765 - 11/20/16 03:03 PM (7 years, 2 months ago) |
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There is no way an 11 year old syringe is going to be sterile in the first place. It's amazing they germinated in the first place, if you have any left I would put it on agar and get it cleaned up.
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Tira



Registered: 11/20/10
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Re: Over 2 months and not even half colonized yet [Re: mushboy]
#23851768 - 11/20/16 03:04 PM (7 years, 2 months ago) |
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I wouldn't toss them as long as the uncolonized area didn't look bacterial (meaning it looks wetter or darker than before).
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Marty Mycfly
Time Traveler


Registered: 12/16/13
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Re: Over 2 months and not even half colonized yet [Re: Tira]
#23851778 - 11/20/16 03:09 PM (7 years, 2 months ago) |
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Out of curiosity, what would you do with jars that haven't colonized in two months, that should have been in 3 weeks or less.
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acidninja
student



Registered: 09/08/16
Posts: 1,693
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Re: Over 2 months and not even half colonized yet [Re: blackout]
#23851803 - 11/20/16 03:18 PM (7 years, 2 months ago) |
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Quote:
blackout said: Post the pics you mentioned. Were the jars ever shaken? I presume you have filters, and know they are OK.
I have also seen a dramatic drop in growth as the cold weather has come in. I think this is normal & expected, but if you read many threads here you will see people saying low temps like 60 or 65F are perfectly fine and just a little slower -many of whom I guess never grew at the temps they mention. They are just reiterating what they heard before, or else do not want to back down after saying the vague "room temperature is fine" line and then find out that persons room is far lower than they would have actually thought.
here are the pics, ive marked the growth for each with a black felt tip on friday:
Jar 1: Golden Teacher
 
Jar 2: Cambodian

Jar 3: Koh Samui

Jar 4: Cambodian
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Over 2 months and not even half colonized yet [Re: blackout]
#23851917 - 11/20/16 03:58 PM (7 years, 2 months ago) |
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Quote:
blackout said:
I have also seen a dramatic drop in growth as the cold weather has come in. I think this is normal & expected, but if you read many threads here you will see people saying low temps like 60 or 65F are perfectly fine and just a little slower -many of whom I guess never grew at the temps they mention.
I have grow in 65F many times. 60 is pretty chilly and I wouldn't recommend it but 65F has worked for me on many occasions. I usually try to keep things at 70-72F of course but in winter in Canada it's pretty tough keeping a basement at 70 all the time.
I don't think the temp ever gets over 72 in my house, I can't stand that. Too hot.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Re: Over 2 months and not even half colonized yet [Re: Pastywhyte]
#23852095 - 11/20/16 04:56 PM (7 years, 2 months ago) |
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Quote:
Pastywhyte said: I have grow in 65F many times. 60 is pretty chilly and I wouldn't recommend it but 65F has worked for me on many occasions.
Yeah, my last few grows were around 60-65F. I did notice it slowing a lot at lower temps though. I put my jars in socks to try and buffer and heat loss from them, and to contain the heat generated by the myc when growing -which I think is highly exaggerated by many, most do not point out that lower temp grows will be slower so the heat generated would be less too.
RR reported colonizing at very low temps
Quote:
RogerRabbit said: I left some pf jars in a cold garage during the winter, when the garage temps were in the 30's and 40's. It took about two months, but they colonized fully. RR
Quote:
RogerRabbit said: Last year, while visiting my brother, I inoculated a bunch of jars for him, thinking he was actually interested. This was in December. I went back in February, and found he didn't have time to bother with them, so put them out in the garage. Temps outside were in the teens and twenties, and in the unheated garage, 30's to low 40's. The jars were fully colonized. I took them home and they fruited like mad. Low temps slow things down, but that's all. I keep master culture slants in the refrigerator for years. RR
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mrmazdarx9
Pffffttt


Registered: 05/15/16
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Re: Over 2 months and not even half colonized yet [Re: blackout]
#23852153 - 11/20/16 05:13 PM (7 years, 2 months ago) |
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They dont look too bad tbh must be a contam in there or something . Ive had my ape in fruiting for two months and only just getting fruits
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blackout


Registered: 07/16/00
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Re: Over 2 months and not even half colonized yet [Re: mrmazdarx9]
#23854176 - 11/21/16 12:29 PM (7 years, 2 months ago) |
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Forgot to say I had presumed they were grain jars for some reason, that's why I was asking if they were shaken. I also missed the bit about 11 year old syringes. The older syringes get the less viable they should be, so less spores will germinate so chances are they will be slower to take off.
You said some started off slow or fast -was this fast to show growth, or fast once growing? how soon after did you see visible growth, with 11 year old syringes I am surprised you got growth with all of them.
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