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cheesie
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B+ agar work from spore print via LC
#23850523 - 11/20/16 05:48 AM (7 years, 2 months ago) |
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So the first appearance of growth.
I am working on black agar as i find it superb for seeing exactly whats on the surface.
Update-

I was wondering what i should do now? I can start to see the edges and i was considering doing further agar plates.
Please advise.
Edited by cheesie (11/22/16 01:49 AM)
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spore-ty



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Re: B+ agar work from spore print via LC [Re: cheesie]
#23850545 - 11/20/16 06:02 AM (7 years, 2 months ago) |
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Looks pretty cool, keep it updated got a log of clones of b+ thatll be up soon
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cheesie
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Re: B+ agar work from spore print via LC [Re: spore-ty]
#23850551 - 11/20/16 06:10 AM (7 years, 2 months ago) |
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Yeah i will do. Have a few plates i had to use the sandwich tek on as there was some contam on the plate, once they start to take off i will pop them on here as well.
One of the plate has there inoculation points but i cant get any decent pics of it yet.
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23850750 - 11/20/16 08:31 AM (7 years, 2 months ago) |
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What do you use to make black agar? What kind of container are you using for that culture?
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cheesie
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Re: B+ agar work from spore print via LC [Re: SampaJasli]
#23850778 - 11/20/16 08:43 AM (7 years, 2 months ago) |
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The containers are oval pp5 food containers. They fit perfectly stacked in the PC i own so no wasted space.
The agar i make myself so i add black food colouring before the agar is PC'd.
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23856159 - 11/22/16 01:56 AM (7 years, 2 months ago) |
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This an update from one of my plates which was from a contaminated plate.
I have just noticed a blemish in the bottom left and i am worried this may be another contamination.
Any advise?
Please.
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23856161 - 11/22/16 02:00 AM (7 years, 2 months ago) |
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Take a small transfer on the other side and go from there man
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23856275 - 11/22/16 04:18 AM (7 years, 2 months ago) |
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Need to knock out some more plates.
If the blemish turns out to be green contam how do i minimise the threat of contamm from this plate?
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23856969 - 11/22/16 11:06 AM (7 years, 2 months ago) |
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Take the transfer now haha it would have turned green already shit in agar shows almost instantly .. if ever you see something pop up do a transfer furthest away from the nastie or if its really fucked and you wanna save the culture do a sandwich by dropping a piece of agar over top the myc will colonize through but the contam wont majority of the time
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23857369 - 11/22/16 01:33 PM (7 years, 2 months ago) |
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I have done three small transfers on a large plate.
Hopefully fingers crossed i will get a result.
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23859422 - 11/23/16 03:44 AM (7 years, 2 months ago) |
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Some of the plates i have ongoing.
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23860495 - 11/23/16 12:38 PM (7 years, 2 months ago) |
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See how the myc is transparent i usually take that if im gstting bacteria on plates
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23860667 - 11/23/16 01:28 PM (7 years, 2 months ago) |
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Are you meaning picture two?
What are your thoughts on the mycelium growth?
Is it normal to have that transparant growth?
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23860736 - 11/23/16 01:45 PM (7 years, 2 months ago) |
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All of them of it im sure your mix may be a bit soft but thats just the growth before it breaks through
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23860814 - 11/23/16 02:10 PM (7 years, 2 months ago) |
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Quote:
noob47 said: All of them of it im sure your mix may be a bit soft but thats just the growth before it breaks through
My mix is literally 1000 ml water 2 table spoons of agar (the chinese supermarket kind) z cut into small pieces 2 table spoons of potato instant mash 1 table spoon of syrup Black food colouring - 8-9 drops
Would you suggest i change the mix?
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23861346 - 11/23/16 05:12 PM (7 years, 2 months ago) |
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2 i would put atleast 10 grams of agar most people recommend 20 to 1000
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23862424 - 11/24/16 01:46 AM (7 years, 2 months ago) |
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So a harder agar would be better?
Also was wondering about lighting.
How much light should the plates be getting?
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23862453 - 11/24/16 02:19 AM (7 years, 2 months ago) |
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I didnt say it was better but two table spoons is like 2.5 grams give or take if you get the sticks of agar like i do there is 2 10gram syicks in a pack ... just saying your agar is really soft not really sure if its good or bad 10 grams os still pretty soft on 1liter i use that on spores ... for lighting i have my agar in the same room as tubs and so they get 12 on 12 off which is beneficial but not a deal breaker
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23862490 - 11/24/16 02:58 AM (7 years, 2 months ago) |
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What type of lighting though? Normal bulbs?
Or would daylight be ok. Was wondering if lighting would make the plates grow better.
I get the sticks of agar.have to cut them up with scissors.
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23862491 - 11/24/16 02:59 AM (7 years, 2 months ago) |
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How do you dissolve all the agar properly?
I find several bits do not dissolve.
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23868633 - 11/26/16 11:14 AM (7 years, 2 months ago) |
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Thoughts?
First pic has some kind of pink contam but im going to do a third transfer.
Second pic the surface seems to have gone pale, maybe some other contam or too dry a surface, not sure??
The rest is new transfers
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23868646 - 11/26/16 11:16 AM (7 years, 2 months ago) |
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This mycelium seems to have stalled and has not grown much in last day or two.
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23869090 - 11/26/16 01:42 PM (7 years, 2 months ago) |
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In the last photo why has the mycelium stalled?
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23869100 - 11/26/16 01:45 PM (7 years, 2 months ago) |
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No it can take 4 or 5 days maybe more for it to leap off just wait it out
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23869662 - 11/26/16 05:00 PM (7 years, 2 months ago) |
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The first two pics should i take a transfer?
Whats the pink stuff on pic 1? I think its contam but not too sure.
Where would you take a transfer from?
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23874183 - 11/28/16 04:38 AM (7 years, 2 months ago) |
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More plates for transfers .
I have knocked up a couple more multi spore plates as well.

This is my lc source and tub with syringe access
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23874989 - 11/28/16 11:44 AM (7 years, 2 months ago) |
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I think that pink shit is bacteria and when you do a transfer try to get the myc on top of the agar like myc side down on top of new plate ..
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23877343 - 11/29/16 06:16 AM (7 years, 2 months ago) |
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One of my transfer plates in close up view
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23877864 - 11/29/16 10:33 AM (7 years, 2 months ago) |
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Looks good man
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23877967 - 11/29/16 11:14 AM (7 years, 2 months ago) |
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Quote:
noob47 said: Looks good man
Thank you.
I have another three transfers from the contam second transfers. All seem to be reaching out already.
I made the agar solution less in syrup and potato flakes as suggested and already it seems to work wonders. Seems to force the mycelium outwards in search for growth nutrients.
I will update more pics as they grow outwards.
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23877971 - 11/29/16 11:15 AM (7 years, 2 months ago) |
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Right on keep it up
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23881434 - 11/30/16 12:14 PM (7 years, 1 month ago) |
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Updated second transfer pics
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Kenetic
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23881712 - 11/30/16 01:35 PM (7 years, 1 month ago) |
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Oh. Thats cute
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cheesie
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Re: B+ agar work from spore print via LC [Re: Kenetic]
#23881737 - 11/30/16 01:44 PM (7 years, 1 month ago) |
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Quote:
kenetic said: Oh. Thats cute
Hey thanks.
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23884258 - 12/01/16 11:31 AM (7 years, 1 month ago) |
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23884292 - 12/01/16 11:48 AM (7 years, 1 month ago) |
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Theres no contam on any of the plates so i was wondering when to sector off and need advice where to sector?
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23884301 - 12/01/16 11:51 AM (7 years, 1 month ago) |
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Look for the fsstest most uniform growth ill take multiple transfers off one plate
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23884313 - 12/01/16 11:56 AM (7 years, 1 month ago) |
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Quote:
noob47 said: Look for the fsstest most uniform growth ill take multiple transfers off one plate
Off the pics which would you use? Diagrams may be useful
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Boogieman47
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23884358 - 12/01/16 12:20 PM (7 years, 1 month ago) |
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If they are def clean id let them grow out to abput a quarter or a little bigger but that first puc id take one off the rhizo and tomentose edge
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cheesie
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Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23884371 - 12/01/16 12:26 PM (7 years, 1 month ago) |
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Will wait patiently then.
I will update pics tommorow
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mycoOrganics
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23884402 - 12/01/16 12:40 PM (7 years, 1 month ago) |
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Quote:
cheesie said: Theres no contam on any of the plates so i was wondering when to sector off and need advice where to sector?
Always a good source of advice....
Edited by mycoOrganics (12/01/16 12:49 PM)
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cheesie
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Re: B+ agar work from spore print via LC [Re: mycoOrganics]
#23884420 - 12/01/16 12:47 PM (7 years, 1 month ago) |
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Thanks. Not sure what pouring agar has with sectoring out mycelium...
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mycoOrganics
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23884424 - 12/01/16 12:48 PM (7 years, 1 month ago) |
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Should be titled strain isolation.. you must have grabbed it before I made edit.
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23884672 - 12/01/16 02:29 PM (7 years, 1 month ago) |
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Ahh thats better. Thanks
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23887232 - 12/02/16 11:10 AM (7 years, 1 month ago) |
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23887238 - 12/02/16 11:12 AM (7 years, 1 month ago) |
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23887241 - 12/02/16 11:13 AM (7 years, 1 month ago) |
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Updated pictures of the daily growth of the mycelium
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spacechildo
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23887260 - 12/02/16 11:24 AM (7 years, 1 month ago) |
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that doesnt look like a good culture to start a grow to me. I would start over.
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cheesie
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Re: B+ agar work from spore print via LC [Re: spacechildo]
#23887301 - 12/02/16 11:38 AM (7 years, 1 month ago) |
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Which ones?
Please may i ask why it doesnt look good? They are second transfers.
The myculium is only small about the size of a peanut
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Edited by cheesie (12/02/16 11:43 AM)
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Kenetic
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23887343 - 12/02/16 11:53 AM (7 years, 1 month ago) |
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Looks fine to me. Id give it anotjer transfer or two since youre new
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cheesie
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Re: B+ agar work from spore print via LC [Re: Kenetic]
#23887350 - 12/02/16 11:56 AM (7 years, 1 month ago) |
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Quote:
kenetic said: Looks fine to me. Id give it another transfer or two since youre new
Should i leave it to grow out some more as they are very small at the minute?
Also where would you advise to take a transfer from and how many?
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spacechildo
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23887456 - 12/02/16 12:36 PM (7 years, 1 month ago) |
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all your pics, the mycelium looks so weak and like its fighting contams, also the fuzzing up in the center is usually a bad sign.
Quote:
cheesie said:

if anything I'd try to take a transfer from 9 o clock on this one. try changing your media/nute source too, see if that helps.
I'm not sure I understand the title here but did you put spores to LC and now trying to clean it up on agar? Rather put spores to agar, clean it up and then put a wedge in a LC if so.
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23887486 - 12/02/16 12:46 PM (7 years, 1 month ago) |
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I did change the nutes from advice. Making the syrup and potato a weaker solution and increasing the agar quantity.
The spores are quite old and they were not germinating at all so i put some in an lc and they flourished and i have been using that to inoculate the agar.
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23887497 - 12/02/16 12:49 PM (7 years, 1 month ago) |
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The pictures are taken through the lids which do not help with the images
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spacechildo
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23887532 - 12/02/16 01:02 PM (7 years, 1 month ago) |
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its still pretty obvious that this plate is riddled with bacteria.
Quote:
cheesie said:

as I said in the previous post, that 1 plate has some decent growth, you can try transferring that. those other plate I wouldn't bother with.
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cheesie
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Re: B+ agar work from spore print via LC [Re: spacechildo]
#23889697 - 12/03/16 04:57 AM (7 years, 1 month ago) |
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cheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
#23889869 - 12/03/16 07:55 AM (7 years, 1 month ago) |
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I have now conducted third transfers onto new plates.
I am wondering if my agar is too mushy as i have struggled to get samples from the precursor plates.
Note to self... harder agar.
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