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InvisiblemycoOrganics
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23884402 - 12/01/16 12:40 PM (7 years, 1 month ago)

Quote:

cheesie said:
Theres no contam on any of the plates so i was wondering when to sector off and need advice where to sector?



Always a good source of advice....


Edited by mycoOrganics (12/01/16 12:49 PM)


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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: mycoOrganics]
    #23884420 - 12/01/16 12:47 PM (7 years, 1 month ago)

Thanks. Not sure what pouring agar has with sectoring out mycelium...


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InvisiblemycoOrganics
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23884424 - 12/01/16 12:48 PM (7 years, 1 month ago)

Should be titled strain isolation.. you must have grabbed it before I made edit.


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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23884672 - 12/01/16 02:29 PM (7 years, 1 month ago)

Ahh thats better. Thanks :smile:


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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23887232 - 12/02/16 11:10 AM (7 years, 1 month ago)



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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23887238 - 12/02/16 11:12 AM (7 years, 1 month ago)



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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23887241 - 12/02/16 11:13 AM (7 years, 1 month ago)

Updated pictures of the daily growth of the mycelium


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Offlinespacechildo
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23887260 - 12/02/16 11:24 AM (7 years, 1 month ago)

that doesnt look like a good culture to start a grow to me. I would start over.


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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: spacechildo]
    #23887301 - 12/02/16 11:38 AM (7 years, 1 month ago)

Which ones?


Please may i ask why it doesnt look good? They are second transfers.

The myculium is only small about the size of a peanut


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Edited by cheesie (12/02/16 11:43 AM)


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OfflineKenetic
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23887343 - 12/02/16 11:53 AM (7 years, 1 month ago)

Looks fine to me.  Id give it anotjer transfer or two since youre new


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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: Kenetic]
    #23887350 - 12/02/16 11:56 AM (7 years, 1 month ago)

Quote:

kenetic said:
Looks fine to me.  Id give it another transfer or two since youre new





Should i leave it to grow out some more as they are very small at the minute?

Also where would you advise to take a transfer from and how many?


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Offlinespacechildo
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23887456 - 12/02/16 12:36 PM (7 years, 1 month ago)

all your pics, the mycelium looks so weak and like its fighting contams, also the fuzzing up in the center is usually a bad sign.

Quote:

cheesie said:





if anything I'd try to take a transfer from 9 o clock on this one.
try changing your media/nute source too, see if that helps.

I'm not sure I understand the title here but did you put spores to LC and now trying to clean it up on agar?
Rather put spores to agar, clean it up and then put a wedge in a LC if so.


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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23887486 - 12/02/16 12:46 PM (7 years, 1 month ago)

I did change the nutes from advice. Making the syrup and potato a weaker solution and increasing the agar quantity.

The spores are quite old and they were not germinating at all so i put some in an lc and they flourished and i have been using that to inoculate the agar.


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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23887497 - 12/02/16 12:49 PM (7 years, 1 month ago)

The pictures are taken through the lids which do not help with the images


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Offlinespacechildo
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23887532 - 12/02/16 01:02 PM (7 years, 1 month ago)

its still pretty obvious that this plate is riddled with bacteria.
Quote:

cheesie said:





as I said in the previous post, that 1 plate has some decent growth, you can try transferring that. those other plate I wouldn't bother with.


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Re: B+ agar work from spore print via LC [Re: spacechildo]
    #23889697 - 12/03/16 04:57 AM (7 years, 1 month ago)



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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23889869 - 12/03/16 07:55 AM (7 years, 1 month ago)

I have now conducted third transfers onto new plates.


I am wondering if my agar is too mushy as i have struggled to get samples from the precursor plates.


Note to self... harder agar.


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