|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23868633 - 11/26/16 11:14 AM (7 years, 2 months ago) |
|
|

Thoughts?
First pic has some kind of pink contam but im going to do a third transfer.
Second pic the surface seems to have gone pale, maybe some other contam or too dry a surface, not sure??
The rest is new transfers
--------------------
issues require tissues
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23868646 - 11/26/16 11:16 AM (7 years, 2 months ago) |
|
|

This mycelium seems to have stalled and has not grown much in last day or two.
--------------------
issues require tissues
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23869090 - 11/26/16 01:42 PM (7 years, 2 months ago) |
|
|
In the last photo why has the mycelium stalled?
--------------------
issues require tissues
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23869100 - 11/26/16 01:45 PM (7 years, 2 months ago) |
|
|
No it can take 4 or 5 days maybe more for it to leap off just wait it out
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23869662 - 11/26/16 05:00 PM (7 years, 2 months ago) |
|
|
The first two pics should i take a transfer?
Whats the pink stuff on pic 1? I think its contam but not too sure.
Where would you take a transfer from?
--------------------
issues require tissues
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23874183 - 11/28/16 04:38 AM (7 years, 2 months ago) |
|
|

More plates for transfers .
I have knocked up a couple more multi spore plates as well.

This is my lc source and tub with syringe access
--------------------
issues require tissues
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23874989 - 11/28/16 11:44 AM (7 years, 2 months ago) |
|
|
I think that pink shit is bacteria and when you do a transfer try to get the myc on top of the agar like myc side down on top of new plate ..
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23877343 - 11/29/16 06:16 AM (7 years, 2 months ago) |
|
|

One of my transfer plates in close up view
--------------------
issues require tissues
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23877864 - 11/29/16 10:33 AM (7 years, 2 months ago) |
|
|
Looks good man
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23877967 - 11/29/16 11:14 AM (7 years, 2 months ago) |
|
|
Quote:
noob47 said: Looks good man
Thank you.
I have another three transfers from the contam second transfers. All seem to be reaching out already.
I made the agar solution less in syrup and potato flakes as suggested and already it seems to work wonders. Seems to force the mycelium outwards in search for growth nutrients.
I will update more pics as they grow outwards.
--------------------
issues require tissues
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23877971 - 11/29/16 11:15 AM (7 years, 2 months ago) |
|
|
Right on keep it up
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23881434 - 11/30/16 12:14 PM (7 years, 1 month ago) |
|
|
Updated second transfer pics
--------------------
issues require tissues
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23881712 - 11/30/16 01:35 PM (7 years, 1 month ago) |
|
|
Oh. Thats cute
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: Kenetic]
#23881737 - 11/30/16 01:44 PM (7 years, 1 month ago) |
|
|
Quote:
kenetic said: Oh. Thats cute
Hey thanks.
--------------------
issues require tissues
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23884258 - 12/01/16 11:31 AM (7 years, 1 month ago) |
|
|
--------------------
issues require tissues
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23884292 - 12/01/16 11:48 AM (7 years, 1 month ago) |
|
|
Theres no contam on any of the plates so i was wondering when to sector off and need advice where to sector?
--------------------
issues require tissues
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23884301 - 12/01/16 11:51 AM (7 years, 1 month ago) |
|
|
Look for the fsstest most uniform growth ill take multiple transfers off one plate
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23884313 - 12/01/16 11:56 AM (7 years, 1 month ago) |
|
|
Quote:
noob47 said: Look for the fsstest most uniform growth ill take multiple transfers off one plate
Off the pics which would you use? Diagrams may be useful
--------------------
issues require tissues
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
Re: B+ agar work from spore print via LC [Re: cheesie]
#23884358 - 12/01/16 12:20 PM (7 years, 1 month ago) |
|
|
If they are def clean id let them grow out to abput a quarter or a little bigger but that first puc id take one off the rhizo and tomentose edge
|
cheesie
supreme friendly being



Registered: 07/03/06
Posts: 902
Last seen: 10 months, 29 days
|
Re: B+ agar work from spore print via LC [Re: Boogieman47]
#23884371 - 12/01/16 12:26 PM (7 years, 1 month ago) |
|
|
Will wait patiently then.
I will update pics tommorow
--------------------
issues require tissues
|
|