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Offlinecheesie
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B+ agar work from spore print via LC
    #23850523 - 11/20/16 05:48 AM (7 years, 2 months ago)



So the first appearance of growth.

I am working on black agar as i find it superb for seeing exactly whats on the surface.




Update-




I was wondering what i should do now? I can start to see the edges and i was considering doing further agar plates.

Please advise.



Edited by cheesie (11/22/16 01:49 AM)


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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23850545 - 11/20/16 06:02 AM (7 years, 2 months ago)

Looks pretty cool, keep it updated got a log of clones of b+ thatll be up soon


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Re: B+ agar work from spore print via LC [Re: spore-ty]
    #23850551 - 11/20/16 06:10 AM (7 years, 2 months ago)

Yeah i will do. Have a few plates i had to use the sandwich tek on as there was some contam on the plate, once they start to take off i will pop them on here as well.

One of the plate has there inoculation points but i cant get any decent pics of it yet.


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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23850750 - 11/20/16 08:31 AM (7 years, 2 months ago)

What do you use to make black agar? What kind of container are you using for that culture?


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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: SampaJasli]
    #23850778 - 11/20/16 08:43 AM (7 years, 2 months ago)

The containers are oval pp5 food containers. They fit perfectly stacked in the PC i own so no wasted space.

The agar i make myself so i add black food colouring before the agar is PC'd.


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Offlinecheesie
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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23856159 - 11/22/16 01:56 AM (7 years, 2 months ago)




This an update from one of my plates which was from a contaminated plate.

I have just noticed a blemish in the bottom left and i am worried this may be another contamination.


Any advise?

Please.


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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23856161 - 11/22/16 02:00 AM (7 years, 2 months ago)

Take a small transfer on the other side and go from there man


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Re: B+ agar work from spore print via LC [Re: Boogieman47]
    #23856275 - 11/22/16 04:18 AM (7 years, 2 months ago)

Need to knock out some more plates.

If the blemish turns out to be green contam how do i minimise the threat of contamm from this plate?


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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23856969 - 11/22/16 11:06 AM (7 years, 2 months ago)

Take the transfer now haha it would have turned green already shit in agar shows almost instantly .. if ever you see something pop up do a transfer furthest away from the nastie or if its really fucked and you wanna save the culture do a sandwich by dropping a piece of agar over top the myc will colonize through but the contam wont majority of the time


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Re: B+ agar work from spore print via LC [Re: Boogieman47]
    #23857369 - 11/22/16 01:33 PM (7 years, 2 months ago)

I have done three small transfers on a large plate.

Hopefully fingers crossed i will get a result.


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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23859422 - 11/23/16 03:44 AM (7 years, 2 months ago)

Some of the plates i have ongoing.




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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23860495 - 11/23/16 12:38 PM (7 years, 2 months ago)

See how the myc is transparent i usually take that if im gstting bacteria on plates


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Re: B+ agar work from spore print via LC [Re: Boogieman47]
    #23860667 - 11/23/16 01:28 PM (7 years, 2 months ago)

Are you meaning picture two?

What are your thoughts on the mycelium growth?

Is it normal to have that transparant growth?


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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23860736 - 11/23/16 01:45 PM (7 years, 2 months ago)

All of them of it im sure your mix may be a bit soft but thats just the growth before it breaks through


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Re: B+ agar work from spore print via LC [Re: Boogieman47]
    #23860814 - 11/23/16 02:10 PM (7 years, 2 months ago)

Quote:

noob47 said:
All of them of it im sure your mix may be a bit soft but thats just the growth before it breaks through




My mix is literally 1000 ml water
2 table spoons of agar (the chinese supermarket kind) z cut into small pieces
2 table spoons of potato instant mash
1 table spoon of syrup
Black food colouring - 8-9 drops


Would you suggest i change the mix?


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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23861346 - 11/23/16 05:12 PM (7 years, 2 months ago)

2 i would put atleast 10 grams of agar most people recommend 20 to 1000


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Re: B+ agar work from spore print via LC [Re: Boogieman47]
    #23862424 - 11/24/16 01:46 AM (7 years, 2 months ago)

So a harder agar would be better?


Also was wondering about lighting.

How much light should the plates be getting?


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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23862453 - 11/24/16 02:19 AM (7 years, 2 months ago)

I didnt say it was better but two table spoons is like 2.5 grams give or take if you get the sticks of agar like i do there is 2 10gram syicks in a pack ... just saying your agar is really soft not really sure if its good or bad 10 grams os still pretty soft on 1liter i use that on spores ... for lighting i have my agar in the same room as tubs and so they get 12 on 12 off which is beneficial but not a deal breaker


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Re: B+ agar work from spore print via LC [Re: Boogieman47]
    #23862490 - 11/24/16 02:58 AM (7 years, 2 months ago)

What type of lighting though? Normal bulbs?

Or would daylight be ok. Was wondering if lighting would make the plates grow better.

I get the sticks of agar.have to cut them up with scissors.


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Re: B+ agar work from spore print via LC [Re: cheesie]
    #23862491 - 11/24/16 02:59 AM (7 years, 2 months ago)

How do you dissolve all the agar properly?

I find several bits do not dissolve.


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