Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds High THC Strains   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineWeavieWonder
Time Travel Sucks
Male User Gallery


Registered: 08/18/16
Posts: 1,455
Loc: USA Flag
Last seen: 1 month, 4 days
Contam free agar plates?
    #23849849 - 11/19/16 08:31 PM (7 years, 2 months ago)

Pulled these B+ cultures out of the fridge about 6 hours before taking these pictures.  Was thinking about making wedges with the plates and inoculating some jars of whole oats in a day or two for an experiment.  To my untrained eyes, they appear to be contaminate free?  I assume tomentose myc is ok for inoculation?  Thank you in advance for your thoughts and opinions.

Plate 1


Plate 2


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery


Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Contam free agar plates? [Re: WeavieWonder]
    #23850030 - 11/19/16 10:15 PM (7 years, 2 months ago)

They look good man. I think you have tomentose growth because you just have a shitton of strains growing together on those plates, further transfers would help with that. But anyway, tomentose growth means nothing, it can become rhizo if conditions change.


Extras: Filter Print Post Top
OfflineAgarStudent
Noob
 User Gallery

Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
Re: Contam free agar plates? [Re: Josex]
    #23850042 - 11/19/16 10:19 PM (7 years, 2 months ago)

Looks good bro :wink:


--------------------
Why reinvent the wheel when someone here has already done the science?



Extras: Filter Print Post Top
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
Re: Contam free agar plates? [Re: AgarStudent]
    #23850226 - 11/19/16 11:58 PM (7 years, 2 months ago)

Rhizo growth comes in with low nutes those look fucking really good i would make some agar up with lower nutes in it to get some rhizo so you can see all the active strains ... id take 3 small tramsfers and drop the rest into grain


--------------------


Extras: Filter Print Post Top
OfflineWeavieWonder
Time Travel Sucks
Male User Gallery


Registered: 08/18/16
Posts: 1,455
Loc: USA Flag
Last seen: 1 month, 4 days
Re: Contam free agar plates? [Re: Boogieman47]
    #23850530 - 11/20/16 05:54 AM (7 years, 2 months ago)

Got a case of 500 petris coming any day now.  Didn't think to make transfers from those before inoculating.  My dumb ass was just gonna start from scratch.  Good suggestion, I will do that.  Will also tinker with my agar recipe.  It's a premix, and am still trying to find the sweet spot, ratio wise.  This time I'll try 40g/L instead of the recommended 50g/L.  Thanks!


Extras: Filter Print Post Top
OfflineAlCapone2k
Beginner
Male

Registered: 10/06/16
Posts: 842
Loc: Germany Flag
Last seen: 6 years, 4 months
Re: Contam free agar plates? [Re: WeavieWonder]
    #23850603 - 11/20/16 06:47 AM (7 years, 2 months ago)

With a premix the problem is, that the Agar will lose thinkness when you decrease the conecentration of the premix. If you are happy with your agar thinkness at the moment, better don't change the concentration. If I were you, I wouldn't change in thinknesss for the sake of tomentose/rhizo growth, cause it's not that important (as I also thought at the beginning).

Next time you better go for buying every ingredient of the agar you favour and mix it yourself! :smile:

Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Extras: Filter Print Post Top
Offlineenlightenment
alchemist
 User Gallery

Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
Re: Contam free agar plates? [Re: AlCapone2k]
    #23850615 - 11/20/16 06:56 AM (7 years, 2 months ago)

:whathesaid:

One advantage of premixed agar is that the plates are clear after pouring without sediment or something (premixed MEA).
If you don't care about sediment or cloudy plates, mixing your agar is cheaper + you can adjust nutrients and agar content.


Extras: Filter Print Post Top
OfflineWeavieWonder
Time Travel Sucks
Male User Gallery


Registered: 08/18/16
Posts: 1,455
Loc: USA Flag
Last seen: 1 month, 4 days
Re: Contam free agar plates? [Re: enlightenment]
    #23851254 - 11/20/16 11:48 AM (7 years, 2 months ago)

Making my own mixes is something I would like to explore in the future.  There are definitely some advantages to doing so.  For the moment, I have a pound of premix, enriched MEA to burn through.  It's a great product, and has served me well so far.  Seems to always set up super stiff at 45 - 50g/L.  I have read that spore germination is faster on a looser growing medium.  Not sure if it really matters for transfers though.  I hope to have all the finer details of agar work figured out by the time I burn through a case of plates.  Experiment time!


Extras: Filter Print Post Top
Offlineenlightenment
alchemist
 User Gallery

Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
Re: Contam free agar plates? [Re: WeavieWonder]
    #23851272 - 11/20/16 11:53 AM (7 years, 2 months ago)

It does not matter for transfers. A higher nutrition content slows the growth a bit. Can you post the detailed ingredients of your premix?


Extras: Filter Print Post Top
OfflineWeavieWonder
Time Travel Sucks
Male User Gallery


Registered: 08/18/16
Posts: 1,455
Loc: USA Flag
Last seen: 1 month, 4 days
Re: Contam free agar plates? [Re: enlightenment]
    #23851386 - 11/20/16 12:36 PM (7 years, 2 months ago)

Per the jar label:  agar, malt, yeast extract, and soy protein.


Extras: Filter Print Post Top
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
Re: Contam free agar plates? [Re: WeavieWonder]
    #23851432 - 11/20/16 12:49 PM (7 years, 2 months ago)

Not sure where you live man but you can buy agar sticks at an asian mart for like 1.30$ for 20 grams ill buy 400 grams at a time then potatoe flakes or malt for cheap the price youre paying for premixed is enough to buy 6 months worth or more of doing it yourself... that dude is right about tomentose and rhizo its not important at all but with rhizo its easier to see sectors and that i do 20 grams agar 10 grams malt with 1000ml and 10 10 to 1000ml for spores


--------------------


Extras: Filter Print Post Top
OfflineWeavieWonder
Time Travel Sucks
Male User Gallery


Registered: 08/18/16
Posts: 1,455
Loc: USA Flag
Last seen: 1 month, 4 days
Re: Contam free agar plates? [Re: Boogieman47]
    #23851521 - 11/20/16 01:20 PM (7 years, 2 months ago)

I have a couple asian markets in my area.  I didn't know agar agar came in 20g sticks.  That's awesome!  Always just seen packaged flakes for way too much money at the grocery.  There are couple of beer brewing places I could check out for malt.  I assume you just drop the stick in water to dissolve?  I'll log that recipe for later use.  Thanks.:thumbup:


Extras: Filter Print Post Top
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
Re: Contam free agar plates? [Re: WeavieWonder]
    #23851590 - 11/20/16 01:46 PM (7 years, 2 months ago)

I blend them in a ninja you can do it in a coffe grinder or just drop them in it takes longer tho and they come in 2 10 gram sticks in a pack in my asian mart the sticks are in a different aisle then the powdered shit but ya my chick bought some for me at whole foodd it was 13 bucks for like 7 grams or someshit ... also those sticks are precolored


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds High THC Strains   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 12,190 3 12/04/99 12:35 AM
by Anonymous
* Whats with my agar plates? Frankenstein 8,600 8 09/08/22 03:46 PM
by schpat
* How many mycelium syringes from one agar plate? BBShrizz 4,884 3 05/10/01 02:10 AM
by ISaToriBluEI
* Liquidplaya's 99% contam free grain -> X TransfTek liquidplaya 2,063 7 10/25/16 10:59 AM
by Greg
* Re: agar plate question Psilowarrior 2,541 2 12/10/99 02:09 PM
by rebelmoon
* Spore Syringe on Agar plates Heruuka 2,929 2 09/16/02 06:31 AM
by Anno
* straw tek...how to keep contam free? NiGGy 3,047 14 01/18/03 06:40 AM
by gee_bone
* Pet Zombies first contam free grow, but!!! gompyre 830 14 12/08/02 09:33 PM
by bugabuga420

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
615 topic views. 18 members, 143 guests and 26 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.005 seconds on 12 queries.