|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
MythObscuro
Stranger

Registered: 11/22/15
Posts: 17
Last seen: 5 years, 8 months
|
Slow LC Growth 1
#23848530 - 11/19/16 12:56 PM (7 years, 2 months ago) |
|
|
This liquid culture was started on October 8th with corn syrup and B+ spores, and seemed to be growing very slowly for the first few weeks, when liquid culture should usually be done within a couple weeks. It was checked about two days ago after having been left alone for a while, and it's completely blossomed.
My question is: Is this a good culture to use?
My concern is that even though it's well developed now, once grain is inoculated with it, it'll continue to grow at this slow rate, not only taking forever but leaving more time for the jars to go sour. Has anyone dealt with this before?
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
|
|
Spores to LC is always bad idea.
Better g2g than using LC if you do not work with agar.
|
Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
|
|
It's toast, probably full of bacteria. Spores to LC is a bad idea.
|
MythObscuro
Stranger

Registered: 11/22/15
Posts: 17
Last seen: 5 years, 8 months
|
Re: Slow LC Growth [Re: Greg]
#23848558 - 11/19/16 01:02 PM (7 years, 2 months ago) |
|
|
It looks perfectly fine to me, just like any other viable LC I've ever made. I used a still air box and took every other precaution I could have with what I had.
|
MythObscuro
Stranger

Registered: 11/22/15
Posts: 17
Last seen: 5 years, 8 months
|
|
It's all I had to work with, and it's worked just fine before. Set up a monotub with that method that fruited magnificently. Not denying that agar is much better, but I'm working with what I've got.
|
A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
|
|
Quote:
MythObscuro said: It looks perfectly fine to me, just like any other viable LC I've ever made. I used a still air box and took every other precaution I could have with what I had.
That's the thing with the LC, it can be hard to tell visually. I only tried a few and never liked it. I wish you the best Dick
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
|
|
Chances are high that spores to LC leads to a contaminated LC. If you want to make an liquid inoculant you can use Grain LC because it is easier to notice contaminations. Before you use your LC test it on one jar to avoid waste of resources and disappointment.
I like to work with LC but it needs proper sterile technique to get successful results.
|
Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
|
|
As others said, it's impossible to tell if you have bacteria in an LC without agar or a microscope. (until it's too late and has fucked a bunch of jars of grain)
Top, extremely bacterial Wine Cap LC. Bottom is healthy Wine Cap LC.
 The bacterial LC only colonizes quarts of rye about halfway then it starts smelling like a pig pen and stalls out.
Left, same bacterial LC as before but on agar. Right, same healthy LC on agar.
  Both of these are actually transfers from the LC plates so there is only a single inoculation point. Makes it easier to see the difference.
|
MythObscuro
Stranger

Registered: 11/22/15
Posts: 17
Last seen: 5 years, 8 months
|
Re: Slow LC Growth [Re: Greg]
#23848629 - 11/19/16 01:21 PM (7 years, 2 months ago) |
|
|
Hm, I see... Looks like i should invest in some agar plates. But for now, I think i'll try inoculating a jar. Thank you for the advice. I wasn't aware that liquid culture could be such a drag.
|
psilly the kid
Hedge wizard/ Cultist



Registered: 05/17/16
Posts: 4,615
Loc: Stoprock City
Last seen: 1 year, 3 months
|
|
the best results I get is from doing the potatoe boil water then use that to do a beer base with 6 row malt and coffee grounds and honey/light corn syrup then dissolve in the agar and your golden. I just use the small jelly jars.. with two fiber plugs and foil. pressure cook it. let it cool and inoculate. and remember you only need a quarter inch of agar when you pour.
-------------------- CA weed @ all times unless specified currently this high pebbles suck its turtles all the way down
|
|