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Offlinedrscouy
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Registered: 11/19/16
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Pinkish and yellowish droplets on surface
    #23847659 - 11/19/16 07:53 AM (7 years, 2 months ago)

I have a 7-day old jar of oyster mycelium which I believe is contaminated, but I can't identify exactly what is going on.



(note that there is an excess of humidity as I forgot to add vermiculite)

Close up, on the surface we can see droplets with a pinkish and yellowish tint:



I would suspect a bacterial contamination, but just wanted to confirm.


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OfflineGreg
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Re: Pinkish and yellowish droplets on surface [Re: drscouy]
    #23849590 - 11/19/16 06:42 PM (7 years, 2 months ago)

Lots and lots of bacteria. The droplets are a mixture of metabolites and bacteria.
Grain doesn't need vermiculite.
What is your filter, how did you sterilize and inoculate the jar?


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Offlinedrscouy
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Re: Pinkish and yellowish droplets on surface [Re: Greg]
    #23850486 - 11/20/16 05:03 AM (7 years, 2 months ago)

Pressure cooked, 2 hours at 15psi, polyfill. G2G transfer inside a still air box. So I suppose the origin of the contamination might have been the grain from which I did the transfer, or for some reason my polyfill filter leaked.


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OfflineGreg
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Re: Pinkish and yellowish droplets on surface [Re: drscouy]
    #23851103 - 11/20/16 10:51 AM (7 years, 2 months ago)

How did the master(s) you G2Gd from look? I bet they had bacteria too.
It could have also been the filter like you said.
I used to use polyfill but I got lots of contams from filter fails with it.


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Offlinedrscouy
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Re: Pinkish and yellowish droplets on surface [Re: Greg]
    #23851831 - 11/20/16 03:25 PM (7 years, 2 months ago)

It looked normal, at least to my eyes. Maybe I'm not good at identifying bacterial contamination.


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