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OfflineBigbadwooof
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Fastest way - Agar to spawn
    #23843781 - 11/17/16 11:06 PM (7 years, 3 months ago)

Any suggestions on quick ways to turn a couple plates into a dozen jars of spawn? I tried making a liquid inoculant, but it's been several days and I don't see any growth. I'm wondering if my new method of grain prep is leaving my grains too dry. I have been playing with the level of hydration, but it takes time.

Anyway, just wondering if anyone has any insights they would like to share. I have considered making a slurry, but I only have a magic bullet, not an Oster, and I don't think I can PC the bottom.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy

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OfflineThe Mycologist
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Re: Fastest way - Agar to spawn [Re: Bigbadwooof]
    #23843790 - 11/17/16 11:10 PM (7 years, 3 months ago)

g2g


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

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InvisibleMoabfighter
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Re: Fastest way - Agar to spawn [Re: Bigbadwooof]
    #23843791 - 11/17/16 11:10 PM (7 years, 3 months ago)

Very much in for research.

I'm PCing jars now, and still on the fence about how to go about using this agar to inoculate...

Considering cooking up a half pint jar, dropping a wedge in, and using that for G2G. It being a half pint, shouldn't take long to colonize.

My biggest idea was the liquid inoculate. You're saying you have no luck with it? I'm also thinking of taking 7 wedges per plate per strain. My plan is to do 3, 7 quart tubs, with  3 variant strains.


--------------------
KSSS And PE WBS.
 

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InvisibleMarty Mycfly
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Re: Fastest way - Agar to spawn [Re: Moabfighter]
    #23843801 - 11/17/16 11:14 PM (7 years, 3 months ago)

Take some wedges to the jars, if you already have some LI jars inoculated your bound to see growth sooner or later, and if contamed you will at least have some proper master jars going.
Always good practice to work on the basics like that.


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Mycfly's King Oysters                                       

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Invisibleazur
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Re: Fastest way - Agar to spawn [Re: Bigbadwooof]
    #23843806 - 11/17/16 11:19 PM (7 years, 3 months ago)

Quote:

Bigbadwooof said:
Any suggestions on quick ways to turn a couple plates into a dozen jars of spawn? I tried making a liquid inoculant, but it's been several days and I don't see any growth. I'm wondering if my new method of grain prep is leaving my grains too dry. I have been playing with the level of hydration, but it takes time.

Anyway, just wondering if anyone has any insights they would like to share. I have considered making a slurry, but I only have a magic bullet, not an Oster, and I don't think I can PC the bottom.



You're new method will be a little slower on growth, but infinitely better at reducing bacteria.
I've found lc to be faster than li.
I use a full plate (just myc growth, not all of myc) when I've got enough and that is fast.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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OfflineBigbadwooof
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Re: Fastest way - Agar to spawn [Re: azur]
    #23843843 - 11/17/16 11:48 PM (7 years, 3 months ago)

Quote:

azur said:
Quote:

Bigbadwooof said:
Any suggestions on quick ways to turn a couple plates into a dozen jars of spawn? I tried making a liquid inoculant, but it's been several days and I don't see any growth. I'm wondering if my new method of grain prep is leaving my grains too dry. I have been playing with the level of hydration, but it takes time.

Anyway, just wondering if anyone has any insights they would like to share. I have considered making a slurry, but I only have a magic bullet, not an Oster, and I don't think I can PC the bottom.



You're new method will be a little slower on growth, but infinitely better at reducing bacteria.
I've found lc to be faster than li.
I use a full plate (just myc growth, not all of myc) when I've got enough and that is fast.




I see, I didn't realize it would be slower, but I'm sure it will cut down on bacteria. They will make great masters, but I need spawn ASAP. Started some LCs on Sunday. Is it normal for it to take this long to see growth from the LI jars?

Also, does anyone know if magic bullet bases can be used for slurries?


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy

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Offlinetump
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Re: Fastest way - Agar to spawn [Re: Bigbadwooof]
    #23844023 - 11/18/16 02:28 AM (7 years, 3 months ago)

No sadly. What makes oyster so useful is the blades can made into lid for regular size mason jar. Benders for li is great and works wonders if pouring correctly. And the grain prep just soak you grains instead of boiling them. Depends on the amount of spawn needed. You say you need a lot quickly. Best advise is li a pint and pour about 15 to 20ml per quart jar. Which you can g2g like normal or if you need the nuclear war amount of spawn you can tru slurrys. If you slurry your better off with small amounts so at the li phase you make 10 master half pint jars of grain which you knock up with your qt jars. Each grain half pints jar of slurry makes 20 qts jars. You can in weeks times turn each qt jars into 20 bags of grains. Go back to master half pints if you want more spawn.

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Invisiblemorty422
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Re: Fastest way - Agar to spawn [Re: Bigbadwooof]
    #23844039 - 11/18/16 02:43 AM (7 years, 3 months ago)

When I was testing clones-I found this method to be pretty time efficient.
This is my own opinion... so... :shrug:

1. Agar plate (with culture of choice) cut into at least 8 pieces. Maybe a bit more. 10-12 wouldn't hurt...
(Transfer one small piece to new plate...You never know what you are about to discover...label appropriately...)

2. Wedges dropped into quart master. (Master jar should contain the same grain as jars that will be inoculated.) For example: If Master jar is WBS-than the jars you are going to pour the master into should also be WBS.

3. Shake "Quart Master" the same day/night after the wedges have been dropped into it.

4. Shake one more time after mycelium has grown to colonize about 50-60% of the available grains. You'll be surprised how fast this will happen... with that many points of growth, it will blow up on you fairly quickly.

5. Wait until full colonization of jar and then G2G to 6 separate jars.
Make sure the jars that will receive the Master grains are filled accordingly- as you are about to add a fairly large amount of 'master grain' to them
(I G2G 6 jars as it is a great amount for a 66qt tub and it will colonize the tub fairly quickly. Introduce fruiting conditions right at spawn. This will allow quicker pinning times and will reduce your Spawn>fruit time substantially. Just ask Pasty...he's seen 5-6 day full colonization times with pins a few days after with this method...)

6. Shake each of your 6 jars immediately after the G2G transfer.

____________________________________

When you have a culture you REALLY like-you can create 12 master jars the same way as above-and get away with inoculating 144 jars the same way...
Don't worry about pushing them to "2 generations" it is not enough time for senility to take hold...

You can then push 5-6 quarts to a 66qt mono *24-28 times* with this methodology.

You'd better make sure it's a culture that you KNOW will produce good yields though... Fruiting 24-28 tubs that WON'T fruit properly would be a pretty big kick in the genitals... *MAKE SURE YOU TEST YOUR CULTURE BEFORE YOU DO THIS*
___________________________________
I found a clone a few months back that produces really well for me...
I slanted it and continue to use it to this day.
When I make a new round of tubs, (I push 20ish tubs at a time) - I use the above Master jar method to create 12 masters and inoculate 144 jars.
I would not advise doing this until you REALLY get your sterile technique down..as you could easily contaminate 144 jars this way. However, I just wanted to let you know my method and how to do it as quickly as possible...

I'm also sort of drunk....and I get tenacious when I'm drunk! :shrug:

G'Day mate!

Hope the above helped!

:cheers:

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OfflineBigbadwooof
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Re: Fastest way - Agar to spawn [Re: morty422]
    #23844068 - 11/18/16 03:12 AM (7 years, 3 months ago)

Thank you fellas, but I have a pretty firm grasp on the basics :wink:

I was just wondering if there was some latest and greatest innovation, but Azure already commented on this thread and didn't give me shit. I think it's time we lit a fire under his ass for some mad sciency instant spawn shit.

... I'm drunk, don't mind me.

Right now I'm turning 10 mostly colonized plates into 12 jars. Hopefully that will get them finished in less than 10 days. Then I can have 2 for G2G and 10 to make 2 tubs. I already have some going, but they are moving slower than fuck.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"There is no need for conspiracy where interests converge" - George Carlin
Every one of you should see this video.
"If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy

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Invisibleazur
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Re: Fastest way - Agar to spawn [Re: Bigbadwooof]
    #23844369 - 11/18/16 07:26 AM (7 years, 3 months ago)

Look up eatyualive's slurry tek


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Invisibleazur
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Re: Fastest way - Agar to spawn [Re: azur]
    #23844435 - 11/18/16 07:55 AM (7 years, 3 months ago)



--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Offlinespacechildo
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Re: Fastest way - Agar to spawn [Re: azur]
    #23844780 - 11/18/16 10:07 AM (7 years, 3 months ago)

several smaller wedges to each jar, and shake shake shake.
and try some LI/blenderless LI at the same time.
waiting for LC to colonize and then inoc'ing grains are never faster than just wedges to grains IME.

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Offlinedextr0
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Re: Fastest way - Agar to spawn [Re: spacechildo]
    #23846502 - 11/18/16 08:09 PM (7 years, 3 months ago)

I don't know if this is anything like what your looking for but its a cool thread:
https://www.shroomery.org/forums/showflat.php/Cat/0/Number/22252599
CORNMEAL PLATE AND
BROWN RICE FLOUR PLATE
LIQUID INOCULATION

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InvisibleMoabfighter
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Re: Fastest way - Agar to spawn [Re: dextr0]
    #23846603 - 11/18/16 08:44 PM (7 years, 3 months ago)

Think this run I'm gonna play it safe and just do a couple small wedges per jar.

I wanted to sector out, blah blah blah. But for my first agar spawn, I'll just play it safe and keep it "simple" leaving as little vectors for error as possible.


Question though. Can I pull wedges off my first plates? As long as I don't grab the center where the spores were? They look fantastic. Extremely rhizo. But... first plates that I transferred from weeks ago.


--------------------
KSSS And PE WBS.
 

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Offlinespacechildo
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Re: Fastest way - Agar to spawn [Re: Moabfighter]
    #23846624 - 11/18/16 08:48 PM (7 years, 3 months ago)

I wouldn't. didnt you say you have 12 other plates ready? use those instead.
do 11 plates to agar, tic tac toe them, pizza slice them, whatvere, and try 1 or 2 LI. blenderless or not.

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InvisiblebodhisattaMDiscordReddit
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Re: Fastest way - Agar to spawn [Re: spacechildo]
    #23846747 - 11/18/16 09:14 PM (7 years, 3 months ago)

out of lazyness I've tiger dropped spore plates into jars before. I don't suggest it at all especially if you have plans to have reasonable success rates

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InvisibleMoabfighter
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Re: Fastest way - Agar to spawn [Re: bodhisatta]
    #23846788 - 11/18/16 09:24 PM (7 years, 3 months ago)

Thanks brother I'll just hang onto the spore plates and wait for pins to clone. No biggie.

I have three great plates of three different strains. Planning to split each plate into 7 jars. Should I just cut the cooonized part, and leave the uncolonized? Some of the agar is going tan, vs the blue agar when I stated. I assume where the myc is consuming all the nutes. Looks symmetrical and normal.


--------------------
KSSS And PE WBS.
 

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Offlinespacechildo
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Re: Fastest way - Agar to spawn [Re: Moabfighter]
    #23846818 - 11/18/16 09:33 PM (7 years, 3 months ago)

uncolonized agar has no place in your grain jars but keep your leading edge, the shiny looking stuff surrounding the myc on your plates.

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InvisibleMoabfighter
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Re: Fastest way - Agar to spawn [Re: spacechildo]
    #23846946 - 11/18/16 10:09 PM (7 years, 3 months ago)

eeeeek. I may be jumping the gun then. Maybe should wait another week to inoculate.... so the agar can finish colonizing.


--------------------
KSSS And PE WBS.
 

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Offlinespacechildo
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Re: Fastest way - Agar to spawn [Re: Moabfighter]
    #23846952 - 11/18/16 10:11 PM (7 years, 3 months ago)

a week? that's a long time. how's it looking so far? how much you got colonized?

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