|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
cannabolic
Stranger
Registered: 11/02/16
Posts: 20
Last seen: 3 years, 8 days
|
Pan. Cyan. needs manure in Agar?
#23844723 - 11/18/16 09:45 AM (7 years, 2 months ago) |
|
|
I was watching the David Barlow video on cultivating Pan. Cyan. He said that they do not grow well on normal agar preps and made a recipe for Malt Dung Agar (MDA). His MDA recipe was as follows:
25 ml water .5g agar agar .5g malt extract .25g horse manure or poultry manure
Has anyone else heard or know from experience if Pan Cyan doesn't like to grow on plain ol' MEA? Is it just better to add manure every time, even for cubes? Would there be any conflict using this instead of 50/50 malt extract and agar agar? It contains, in addition to malt extract and agar, dextrose and peptone g.
|
spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: cannabolic]
#23844726 - 11/18/16 09:47 AM (7 years, 2 months ago) |
|
|
You dont NEED to but you can train your mycelium to eat wierd things this way.
But no it should eat up that MEA
I'd do your recipe the plate away from transfers to masters
|
cannabolic
Stranger
Registered: 11/02/16
Posts: 20
Last seen: 3 years, 8 days
|
Re: Pan. Cyan. needs manure in Agar? [Re: spore-ty]
#23844745 - 11/18/16 09:56 AM (7 years, 2 months ago) |
|
|
I think I'll try both ways just to see for myself. If there is no noticeable difference I'd prefer to handle the manure only when I need substrate. What did you mean by "I'd do your recipe the plate away from transfers to masters?"
Quote:
spore-ty said: You dont NEED to but you can train your mycelium to eat wierd things this way.
But no it should eat up that MEA
I'd do your recipe the plate away from transfers to masters
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: cannabolic]
#23844756 - 11/18/16 10:02 AM (7 years, 2 months ago) |
|
|
Quote:
cannabolic said: I was watching the David Barlow video on cultivating Pan. Cyan. He said that they do not grow well on normal agar preps
then stop watching his videos. there's a ton of bad info out there, once you see something as wrong as this just stop viewing.
|
cannabolic
Stranger
Registered: 11/02/16
Posts: 20
Last seen: 3 years, 8 days
|
Re: Pan. Cyan. needs manure in Agar? [Re: spacechildo]
#23844777 - 11/18/16 10:06 AM (7 years, 2 months ago) |
|
|
I needed to confirm whether or not his claim was true. So you're saying I shouldn't even bother trying out the manure and go straight for the MEA with Pan Cyan as if it were a cube?
Quote:
spacechildo said:
Quote:
cannabolic said: I was watching the David Barlow video on cultivating Pan. Cyan. He said that they do not grow well on normal agar preps
then stop watching his videos. there's a ton of bad info out there, once you see something as wrong as this just stop viewing.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: cannabolic]
#23844784 - 11/18/16 10:09 AM (7 years, 2 months ago) |
|
|
yes.
|
Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
|
Re: Pan. Cyan. needs manure in Agar? [Re: spacechildo]
#23844787 - 11/18/16 10:11 AM (7 years, 2 months ago) |
|
|
Quote:
spacechildo said:
Quote:
cannabolic said: I was watching the David Barlow video on cultivating Pan. Cyan. He said that they do not grow well on normal agar preps
then stop watching his videos. there's a ton of bad info out there, once you see something as wrong as this just stop viewing.
as wrong as this? Whats so wrong with adding more diverse nutes to agar? Per example, RR adds coffee to agar for sclerotia producers.
At least I thought it sounded like a good idea at first glance. I never did well with pans on agar, though that was likely for other reasons.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: cannabolic]
#23844798 - 11/18/16 10:14 AM (7 years, 2 months ago) |
|
|
that's not what I said, I said this is dead wrong:
Quote:
cannabolic said: they do not grow well on normal agar preps
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Pan. Cyan. needs manure in Agar? [Re: spacechildo]
#23844799 - 11/18/16 10:14 AM (7 years, 2 months ago) |
|
|
i dont like that brit anyway.
at 0:50 he plugs a non vendor. ass.
i think eatualive posted a pic of pan cyan myc on a pasty plate. no poop in those. 
good resource
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: mushboy]
#23844807 - 11/18/16 10:17 AM (7 years, 2 months ago) |
|
|
I searched that dudes name and all I could find was his own youtube videos and a 40page leaflet he tries to sell for $20. sounds like a complete ass just looking to make money with no consideration to the bad advice he's spreading.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Pan. Cyan. needs manure in Agar? [Re: spacechildo]
#23845084 - 11/18/16 12:03 PM (7 years, 2 months ago) |
|
|
Mea works great for pans.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
|
Re: Pan. Cyan. needs manure in Agar? [Re: cronicr]
#23845090 - 11/18/16 12:06 PM (7 years, 2 months ago) |
|
|
Pan. cyan. pinning on MEA:

P.S. in a binomial name, species name is always lowercase & genus is always uppercase.
Edited by Greg (11/18/16 12:11 PM)
|
Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 8 days
|
Re: Pan. Cyan. needs manure in Agar? [Re: Greg]
#23845359 - 11/18/16 02:11 PM (7 years, 2 months ago) |
|
|
Quote:
Greg said: Pan. cyan. pinning on MEA:

P.S. in a binomial name, species name is always lowercase & genus is always uppercase.
What you refer to as the species name is actually the specific epithet. The species name includes the genus and specific epithet, in that order.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
|
GlitchInSpace
Glitch In Space


Registered: 11/24/13
Posts: 426
Loc: Texas
|
|
panaeolus grows great on mea or pda, no manure needed on agar.
|
Panarchist
Cyanescendant



Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 7 months, 17 days
|
Re: Pan. Cyan. needs manure in Agar? [Re: Greg]
#23846368 - 11/18/16 07:26 PM (7 years, 2 months ago) |
|
|
i finally got some MEA recently. the growth on it seems to be better than any of the diy agar recipes i tried. 11 days ago i inoculated some pastyplates with some pan cyan spores and it's already got some mycelial growth that's about the size of a penny on two plates. i think they will be ready for taking some transfers this weekend.
and what is up with that Barlow dude? his teks suck and he doesn't look like he's ever even eaten a single magic mushroom in his entire life.
--------------------
Edited by Panarchist (11/18/16 07:29 PM)
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Pan. Cyan. needs manure in Agar? [Re: Panarchist]
#23846436 - 11/18/16 07:50 PM (7 years, 2 months ago) |
|
|
Simply not listening to anything David Barlow has to say makes you a better cultivator
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: bodhisatta]
#23846458 - 11/18/16 07:55 PM (7 years, 2 months ago) |
|
|
Quote:
bodhisatta said: Simply not listening to anything David Barlow has to say makes you a better cultivator
who is that dude? I've never heard of him until today..
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Pan. Cyan. needs manure in Agar? [Re: spacechildo]
#23846466 - 11/18/16 07:57 PM (7 years, 2 months ago) |
|
|
some weird old british dude that sold DMT on silk road a long time ago he's not a total dumbass but his cultivation videos are kind of useless
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: bodhisatta]
#23846491 - 11/18/16 08:05 PM (7 years, 2 months ago) |
|
|
one of "those other sites" says he's dead. good riddance?
|
Eolus
Stranger
Registered: 07/13/18
Posts: 25
Last seen: 5 years, 2 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: Panarchist] 1
#25345300 - 07/24/18 09:33 PM (5 years, 6 months ago) |
|
|
Quote:
Panarchist said: i finally got some MEA recently. the growth on it seems to be better than any of the diy agar recipes i tried. 11 days ago i inoculated some pastyplates with some pan cyan spores and it's already got some mycelial growth that's about the size of a penny on two plates. i think they will be ready for taking some transfers this weekend.
and what is up with that Barlow dude? his teks suck and he doesn't look like he's ever even eaten a single magic mushroom in his entire life.
I think his rather thorough. He shared the information freely.
How exactly does someone look that's eaten one psilocybin containing mushroom in his life? This some kind of trendy status thing that makes one cool?
|
Dr. Psilocybin
Loner


Registered: 04/08/17
Posts: 17
Last seen: 8 months, 18 days
|
Re: Pan. Cyan. needs manure in Agar? [Re: Eolus] 1
#25345607 - 07/25/18 06:34 AM (5 years, 6 months ago) |
|
|
Right now I have about 14 pasty plates with PDA all growing pan cyan Jamaican mycelium. The myc seems happy as can be and had no trouble growing out on it. Spores I swabbed on the plates (print taken in Feb 2018) all germinated within 3-6 days.
This PDA recipe is just copied from pasty’s easy agar thread, and it worked great for my pans:
1 Tsp powdered agar (5 mL & ~2.5g) 2 Tsp potato flakes (10 mL) 1/2 cup water 1-2 drops of raw natural honey 1-2 Drops food coloring of choice
I made sure to stir the potato flakes into hot water as the first step, in order to dissolve as much from it as possible to help with the issue of all the flakes settling on the bottom. I also adjusted my agar to be slightly softer (less agar powder) because I heard somewhere that pan myc likes softer agar (don’t take as truth, I just read it on here somewhere).
Results may vary.
|
Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
|
|
Man, you guys are tough on that poor old dude. I like him. Mind you, i sat there and watched him contaminate his project, but he did get a few flushes. I mean, he showed how he did it and at the end he had a pan of pans. So, if you're just looking to get some for consumption then i dont see the problem.
Sorry, ive watched that video for years and i just like the old bastard. I mean, he's one of us.
Also, David didnt say they wont grow on malt extract agar, he said They dont perform well on malt agar alone. He then says its not known what makes horse manure so beneficial, as there is very little scientific research in this area.
I just knocked up two dishes with pan cope bisporus and i added powered horse manure. We'll see how it plays out.
Edited by Ghostveil25 (08/15/18 06:41 PM)
|
Milehighru
moderately experienced

Registered: 06/28/17
Posts: 45
|
Re: Pan. Cyan. needs manure in Agar? [Re: Ghostveil25]
#25406497 - 08/23/18 01:08 AM (5 years, 5 months ago) |
|
|
So I started some spores from a print and swabbed to MAE...I just saw on mushrooms.com that they say not to MEA... Obviously you all are using it...anyone know why they are saying not to?
|
Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
|
Re: Pan. Cyan. needs manure in Agar? [Re: Milehighru]
#25406540 - 08/23/18 02:24 AM (5 years, 5 months ago) |
|
|
Quote:
Milehighru said: So I started some spores from a print and swabbed to MAE...I just saw on mushrooms.com that they say not to MEA... Obviously you all are using it...anyone know why they are saying not to?
From what ive read, Pan mycelium doesnt do well on Malt agar alone and that manure must be added for them to germinate. I saw it on YouTube with the David Barlow guy and also on the website you mentioned. However, i came here and numerous trusted cultivators say that its not true. I can say that my dishes with manure added exploded with growth in just 3 days. The regular malt agar plates are just now showing a little cloud of germination. If done correctly i honestly dont think it can hurt. I mean, in the David barlow video, he does use this technique and he does produce a tray of very healthy pans. So, i guess the manure speeds things up, but is not absolutely necessary.
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 16 hours
|
Re: Pan. Cyan. needs manure in Agar? [Re: Ghostveil25]
#25406689 - 08/23/18 05:34 AM (5 years, 5 months ago) |
|
|
I've never used anything but MEA or PDA for Pan species. I have had a better germination rate for Pans on agar than Cubes actually. The agar is just a 2D surface for isolating clean myc and a jumping off pint. Once on grains , it doesn't care what type of agar it was on.
If you have trouble with germination, and the spores are good, sometimes you just need to fiddle with stiffness , nutrients etc.
Its a cool idea adding Hpoo none the less
--------------------
 Amateur Mycologists United AMU Q&A
|
TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
|
Re: Pan. Cyan. needs manure in Agar? [Re: mushpunx]
#25406768 - 08/23/18 06:41 AM (5 years, 5 months ago) |
|
|
I find pan spores to germinate faster with a bit of warmth. BUT this is also a double edge knife in that, extra warmth also speeds bacteria growth. I try to shoot for low to mid 70's, like 72-75 for where my pan germination plates set. Actually this is what I do for anything exotic that likes it warm.
-------------------- JOIN THE POW WOW
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
|
Ive seen people's mea plates with pans forming pins
|
Eolus
Stranger
Registered: 07/13/18
Posts: 25
Last seen: 5 years, 2 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: Ghostveil25]
#25409142 - 08/24/18 04:08 AM (5 years, 5 months ago) |
|
|
Quote:
Ghostveil25 said: I just knocked up two dishes with pan cope bisporus and i added powered horse manure. We'll see how it plays out.
How are these doing?
|
Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
|
Re: Pan. Cyan. needs manure in Agar? [Re: Eolus] 1
#25409266 - 08/24/18 06:14 AM (5 years, 5 months ago) |
|
|
Quote:
Eolus said:
Quote:
Ghostveil25 said: I just knocked up two dishes with pan cope bisporus and i added powered horse manure. We'll see how it plays out.
How are these doing?
Well, i just did transfers on my others but ive left this one alone because it looks great and is spreading out very evenly.  Its a terrible picture but you can see the circle moving out. Dish is very clean with no signs of bacteria. One of the other jars had possible bacteria, but not sure. I transfered it anyway just to be sure.
The mycelium shown here looks good, but theres many gentics here. So, plan to grow out and clone the best. Ive only ever done one other Orissa clone and it was a 100% success.
|
Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
|
Re: Pan. Cyan. needs manure in Agar? [Re: Ghostveil25]
#25416484 - 08/27/18 09:51 AM (5 years, 5 months ago) |
|
|
Well, this one dish is almost ready for grains. Pan bisporus  So, im not sure if the manure has anything to do with the speed of colonization.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Pan. Cyan. needs manure in Agar? [Re: Ghostveil25]
#25416553 - 08/27/18 10:24 AM (5 years, 5 months ago) |
|
|
ive grown pan myc on brf, lme and pda. it all grew the same for me
brf agar(pan on the left)

pda

light malt

the brf and lme pinned as well
|
Nitrogoxygen
Timelord

Registered: 02/19/18
Posts: 533
Loc: A.N.U.S.
Last seen: 4 years, 8 months
|
Re: Pan. Cyan. needs manure in Agar? [Re: mushboy]
#25416623 - 08/27/18 11:08 AM (5 years, 5 months ago) |
|
|
Good to know I just made 8 light malt agar plates inoculated with cyanescens (first spore print)
|
Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
|
Re: Pan. Cyan. needs manure in Agar? [Re: mushboy]
#25416728 - 08/27/18 12:21 PM (5 years, 5 months ago) |
|
|
Quote:
mushboy said: ive grown pan myc on brf, lme and pda. it all grew the same for me
brf agar(pan on the left)

pda

light malt

the brf and lme pinned as well
This is good to know because i didnt like purtung the manure in there afraid of contams, but i guess it wont contam more than anything else. Just an added step.
|
Sulik
Seeker


Registered: 10/17/13
Posts: 15
Loc: Tasmania
Last seen: 2 months, 12 days
|
Re: Pan. Cyan. needs manure in Agar? [Re: Ghostveil25]
#27406936 - 07/29/21 10:53 AM (2 years, 5 months ago) |
|
|
I like David Barlow and his detailed grow tek for Pan Cyans. He has passed away which is a shame, as you say he is another person out there trying to help others and he does grow a nice tray of Pans. I’ve grown Pan spores of PDA but will try adding some horse manure next time and do a comparison for people. You never know the final mushroom may be more potent when raised on agar with manure compared to those raised on plain MEA agar. As you know with most living things, the best nutrition you give at at an early stage results in most cases a strong and healthy adult. Cheers from Australia👍😃
|
|