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Invisibleegobrain


Registered: 11/17/16
Posts: 180
Loc: Canada Flag
Straw/Poo Substrate (uneven colonization)
    #23842695 - 11/17/16 05:26 PM (7 years, 2 months ago)

Hey guys,

I've been lurking for a couple months and have started my second attempt at growing. First time I used a crap grow kit which contaminated during casing colonization. They replaced my kit for a small fee. This time I only used the jar of colonized grain spawn and dry straw they sent me.

I went out and bought some composted cow manure and mixed it with the straw at a 1:1 ratio. Pasteurized in a pillowcase at 160 f. for 90 minutes. Let drain until the next day. Mixed it all up with the grain spawn in the tub at a 5:1 ratio. I might not have mixed everything thoroughly enough and I think this is why my sub is not colonizing evenly. It has progressed in the past 24 hours though, so I don't think its contaminated.

I copied part of Doc P's hybrid mono chamber. I didn't tape my tub though. The sub gets indirect daylight through a window. Its wrapped in cling wrap (loosely on the bottom for gas exchange). It was placed in a room with a constant ambient temp. of 70 f. on the 10th of this month.

Should I be worried about this patch not colonizing evenly? :ooo:

TL;DR
I might not have mixed my straw/poo and spawn thoroughly enough. I think this is why my sub has some uneven colonization. Would like someone to agree that this isn't a contamination.

Pics:




Thanks for the read! :thumbup::mushroom2:


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: Straw/Poo Substrate (uneven colonization) [Re: egobrain]
    #23842763 - 11/17/16 05:42 PM (7 years, 2 months ago)

The thick growth on the edges and the extremely bumpy surface tells me it's battling bacteria right now, and is very stressed. You just left manure and straw hanging in a pillow case overnight? You didn't wring the pillow case out at all? Feel for field capacity or anything? Poo holds so much water it isn't even funny. It's a bacterial land mine when done like that.

Also willing to bet there's some bacteria in the spawn as well.. I wonder if it will even colonize that spot. If it does, try fruiting it. Just next time work on the spawn/inoculant and prep of substrate a little better. I do poo and straw separately, and mix them together after pasteurization.


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Invisibleegobrain


Registered: 11/17/16
Posts: 180
Loc: Canada Flag
Re: Straw/Poo Substrate (uneven colonization) [Re: Mad Season]
    #23842799 - 11/17/16 05:49 PM (7 years, 2 months ago)

I checked it for field capacity after trying to squeeze water out. It seemed fine to me. I actually thought it might have been too dry.

I forgot to mention something critical, actually. The spawn was excreting metabolites in the jar. It smelled like mushrooms though, so I thought that it had a chance. I have spores coming in the mail any day now anyway. Its all just a learning experience. I'm just going to leave it for a few days and watch the progress. If I can get it to fruit, even better.

Thanks for the insight though. I didn't know the thickness and edge growth could indicate the mycelium was battling bacteria. Cool! :cool:


Edited by egobrain (11/17/16 05:52 PM)


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InvisibleMad Season
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Registered: 09/16/12
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Re: Straw/Poo Substrate (uneven colonization) [Re: egobrain]
    #23842841 - 11/17/16 05:59 PM (7 years, 2 months ago)

Ah that sounds good. It should feel "a little too dry" when manure is involved. And yes mycelium and fruit bodies react to almost everything. Metabolites in a jar before spawning definitely doesn't sound good...

Mycelium tells you if things are going well or not. If you haven't, you should look into agar a little bit for those incoming spores :wink:. It really helps to know you're at least inoculating grains with bacteria/contaminant free mycelium doncha think? :wink:


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Invisibleegobrain


Registered: 11/17/16
Posts: 180
Loc: Canada Flag
Re: Straw/Poo Substrate (uneven colonization) [Re: Mad Season]
    #23842877 - 11/17/16 06:07 PM (7 years, 2 months ago)

That's a great idea. I just need to do a little more reading..

I'm building a SAB right now. I will be doing pf tek in a SGFC. I think I'll have more luck there with my lack of experience.


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InvisibleMad Season
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Re: Straw/Poo Substrate (uneven colonization) [Re: egobrain]
    #23842894 - 11/17/16 06:11 PM (7 years, 2 months ago)

Do whatever you want :P. Honestly though I went straight for bulk when I started. Still have yet to do pftek. But you can also get some killer results with pf tek. Really thinking about trying it out soon tbh.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Invisibleegobrain


Registered: 11/17/16
Posts: 180
Loc: Canada Flag
Re: Straw/Poo Substrate (uneven colonization) [Re: Mad Season]
    #23842921 - 11/17/16 06:20 PM (7 years, 2 months ago)

I thought the yield would be limited from the lack of nutes. Wouldn't it be better to just do grains and spawn to bulk? I had impression that pf tek was mostly done because it eliminates some chances of contam (pasteurizing and casing).


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InvisibleMad Season
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Registered: 09/16/12
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Re: Straw/Poo Substrate (uneven colonization) [Re: egobrain]
    #23842941 - 11/17/16 06:26 PM (7 years, 2 months ago)

Yes I went from spawn to bulk, and never did pf tek. I later had insane contamination issues, and had to adopt agar in as my grain inoculant so I could clean my dirty ass spores.

Pf tek is perhaps more easier and less hassle, but the yield is indeed lower overall. Not exactly lacking tho. I've seen some drool worthy results from cakes..



Quote:

TheMadHatter420 said:
Harvested these today. Used some bottom watering.







Etc lol


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisibleegobrain


Registered: 11/17/16
Posts: 180
Loc: Canada Flag
Re: Straw/Poo Substrate (uneven colonization) [Re: Mad Season]
    #23845963 - 11/18/16 05:15 PM (7 years, 2 months ago)

Those are some nice looking trays man! I'll get there eventually..  :meanlaugh:

This is the progression from the last 20 hours. I marked red sharpie right on the edge of where it had colonized to yesterday.


I think the mycelium is fighting the bacteria, but I'd like someones opinion on whether they think this is a lost cause..


Edited by egobrain (11/18/16 05:16 PM)


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