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OfflineSpiggle
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Registered: 03/03/16
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Agar looking good?
    #23840724 - 11/17/16 05:17 AM (7 years, 2 months ago)

Hey guys, I've been transferring a strain from a multispore inoculation i made on agar, i've made three successful transfers so far and i have a couple questions

1. When is the best time to make the transfer? when i see a good sector or before the mycelium may weaken?
2. Does this look like i'm doing the right thing so far?

Thanks for any input and the help! 


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Offlineenlightenment
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Re: Agar looking good? [Re: Spiggle]
    #23840733 - 11/17/16 05:23 AM (7 years, 2 months ago)

Looks OK. Is the "dot" at about 6 o'clock on the inside?
Could be a contam.
You should transfer now.
Cut out a small peace and make another transfer.



I marked which parts I would transfer.

The red circled spot doesn't look good if it is on the inside. Can't tell it by the picture.


Edited by enlightenment (11/17/16 05:32 AM)


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OfflineSpiggle
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Re: Agar looking good? [Re: enlightenment]
    #23840742 - 11/17/16 05:32 AM (7 years, 2 months ago)

Ah yeah that is some kind of bacteria possibly? how does the bacteria contam same as spores? through the air or would likely have been passed on by me rather than the environment.


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Offlineenlightenment
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Re: Agar looking good? [Re: Spiggle]
    #23840749 - 11/17/16 05:36 AM (7 years, 2 months ago)

There are a lot of spores in the air.
Did you use a SAB(still air box)?


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Invisibleblindingleaf
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Re: Agar looking good? [Re: Spiggle]
    #23840750 - 11/17/16 05:36 AM (7 years, 2 months ago)

whats that blue mushroom in ur signature on the right?


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineSpiggle
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Re: Agar looking good? [Re: Spiggle]
    #23840751 - 11/17/16 05:36 AM (7 years, 2 months ago)

can i still save this fungi?


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OfflineSpiggle
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Re: Agar looking good? [Re: blindingleaf]
    #23840752 - 11/17/16 05:37 AM (7 years, 2 months ago)

Stropharia Aeruginosa


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Offlineenlightenment
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Re: Agar looking good? [Re: blindingleaf]
    #23840753 - 11/17/16 05:39 AM (7 years, 2 months ago)

Quote:

blindingleaf said:
whats that blue mushroom in ur signature on the right?




Stropharia aeruginosa

Quote:

Spiggle said:
can i still save this fungi?




IMO it isn't worth the effort but it is possible.


Edited by enlightenment (11/17/16 05:40 AM)


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OfflineSpiggle
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Re: Agar looking good? [Re: enlightenment]
    #23840758 - 11/17/16 05:41 AM (7 years, 2 months ago)

Yeah i used a still air box for this, my sterile technique isn't all that great at the moment first time with agar, do you think its okay to inoculate on grain already?


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OfflineSpiggle
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Re: Agar looking good? [Re: Spiggle]
    #23840762 - 11/17/16 05:46 AM (7 years, 2 months ago)

I'm using standard tape to seel the dishes once transferred is that okay? no contaminants to worry about?  it looks like the bacteria on the bad dish may have got in the sides.


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Offlineenlightenment
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Re: Agar looking good? [Re: enlightenment]
    #23840766 - 11/17/16 05:48 AM (7 years, 2 months ago)

Because of the marked spot I can not recommend to transfer to grain now. It could work but better safe than sorry. Give it one more transfer and wait for some growth to inoculate some grain.
Update this thread or send me a pm before you inoculate.

Try to get some parafilm or at least PE cling film to wrap the sides.


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OfflineSpiggle
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Re: Agar looking good? [Re: enlightenment]
    #23840807 - 11/17/16 06:29 AM (7 years, 2 months ago)

Thanks mate, ill make some further transfers and give you a heads up when i think about grain inoculation! thanks for the help thus far :smile:


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