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OfflineJayo Peso
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Registered: 07/12/13
Posts: 19
Last seen: 8 years, 1 month
agar plate not pc'd fast enough
    #23839362 - 11/16/16 04:18 PM (8 years, 2 months ago)

Yesterday I made a bunch of pasty plates, but then I had to leave the house and didn't get back in time to pc them yesterday, and now upon inspection I see a bit of white growth growing on surface. So I'm wondering should I just trash them and star over or will the pc kill everything living, and they'll be fine to work with?

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OfflineTmethylM
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Registered: 07/16/12
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Loc: Florida
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Re: agar plate not pc'd fast enough [Re: Jayo Peso]
    #23839371 - 11/16/16 04:21 PM (8 years, 2 months ago)

They will be sterilized and nothing alive will be left after PC'ing,
however those white spots will likely remain and will keep you confused about future contaminations.
I would personally re-use them, but some would restart, up to you.
Either way will be fine.(<-- this sentence based on actual experience)


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¯\_(ツ)_/¯

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OfflineJayo Peso
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Re: agar plate not pc'd fast enough [Re: Jayo Peso]
    #23839384 - 11/16/16 04:24 PM (8 years, 2 months ago)

Cool thanks

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OfflineTmethylM
Smear in the shale
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Re: agar plate not pc'd fast enough [Re: Jayo Peso]
    #23839394 - 11/16/16 04:28 PM (8 years, 2 months ago)

Don't forget to wait for a Trusted Cultivator.
I am not worthy of a "cool thanks" as if this is case-closed.

:lol:

Also, out of pure curiosity;
Is the white growth a sort of slime? Or small white scattered spots? Or is it slightly fuzzy?
What else can you see?
I must know.


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¯\_(ツ)_/¯

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OfflineKenetic
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Re: agar plate not pc'd fast enough [Re: Tmethyl]
    #23839448 - 11/16/16 04:45 PM (8 years, 2 months ago)

I agree with the above poster (who btw has been a trustworthy adviser for years) in that the p.c will kill the mold, the only problem I see is that the mold will still be there after the pc and may make it harder to see other stuff on the plates. 

I'd just do it again.

Edit:  yeah, could be mold or bacteria, I see you didn't specify.  The most common bacteria you see is usually yellowish though.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



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OfflineJayo Peso
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Registered: 07/12/13
Posts: 19
Last seen: 8 years, 1 month
Re: agar plate not pc'd fast enough [Re: Kenetic]
    #23840361 - 11/16/16 11:05 PM (8 years, 2 months ago)

Well the spots were white, they resembled myc spots. I went ahead and pc'd after posting, so I guess it will be fine or worst case scenario an interesting experiment. Really that's what all this shit is about anyway right?

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OfflineTmethylM
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Re: agar plate not pc'd fast enough [Re: Jayo Peso]
    #23840505 - 11/17/16 12:55 AM (8 years, 2 months ago)

:nodofunderstanding:


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¯\_(ツ)_/¯

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