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OfflineLogicaL ChaosM
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Re: Grr.. what the hell is this? [Re: azur]
    #23840652 - 11/17/16 03:48 AM (7 years, 2 months ago)

Quote:

azur said:
Bacteria. Try antibiotic agar




This is real?!

:mindblown:


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OfflineThe Mycologist
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Re: Grr.. what the hell is this? [Re: LogicaL Chaos]
    #23840794 - 11/17/16 06:14 AM (7 years, 2 months ago)

Yeah they use aquarium antibiotics. But it seems a lot of people don't like it.


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OfflineLogicaL ChaosM
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Re: Grr.. what the hell is this? [Re: The Mycologist]
    #23841095 - 11/17/16 09:51 AM (7 years, 2 months ago)

Best to not use antibiotic agar huh? I could see that.

I would argue that being super sterile around and with regular agar to prevent infections.


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InvisibleMoabfighter
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Re: Grr.. what the hell is this? [Re: LogicaL Chaos]
    #23841104 - 11/17/16 09:56 AM (7 years, 2 months ago)

Not to disagree with Azur, but my thought is if your agar is so bad you need antibiotic agar, you might as well just start over. Sorry to be blunt, but you obviously weren't trying hard enough.


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InvisibleMad Season
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Re: Grr.. what the hell is this? [Re: Moabfighter] * 3
    #23841129 - 11/17/16 10:07 AM (7 years, 2 months ago)

What azur meant was if his spore source is bacterial, use antibacterial agar, and if op did things as right as he says, the bacteria is undoubtedly because of the syringe.

There's many people I know (kizzle especially swears by this method) who either germs or transfers to antibacterial agar at least once in case there's any hidden bacteria growing with the mycelium.


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InvisibleMoabfighter
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Re: Grr.. what the hell is this? [Re: Mad Season]
    #23841132 - 11/17/16 10:08 AM (7 years, 2 months ago)

Alright I apologize for bad information then. Disregard. Sorry.


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Invisiblecrackbaby
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Re: Grr.. what the hell is this? [Re: Moabfighter]
    #23841158 - 11/17/16 10:17 AM (7 years, 2 months ago)

i've gotten plenty of dud syringes, which is what seems to be the case here.  It took a while racking my brain trying to figure out what i was doing wrong before finally realizing that half the syringes i was getting from a certain vendor were dead.  Once i switched to a reliable vendor it was smooth sailing.  I highly recommend sporeworks.


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Invisibleazur
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Re: Grr.. what the hell is this? [Re: crackbaby]
    #23841176 - 11/17/16 10:24 AM (7 years, 2 months ago)

I had an ape syringe that would not put out anything but bacteria. Finally I put it on antibiotic agar and sandwiched it with antibiotic agar. Clean my grew through and I very easily obtained a pure culture.


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OfflineLogicaL ChaosM
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Re: Grr.. what the hell is this? [Re: Mad Season]
    #23841294 - 11/17/16 11:05 AM (7 years, 2 months ago)

Quote:

Mad Season said:
What azur meant was if his spore source is bacterial, use antibacterial agar, and if op did things as right as he says, the bacteria is undoubtedly because of the syringe.

There's many people I know (kizzle especially swears by this method) who either germs or transfers to antibacterial agar at least once in case there's any hidden bacteria growing with the mycelium.




Im dumbfounded vendors even sell baterial syringes. blows my mind.


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OfflinecronicrFacebook
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Re: Grr.. what the hell is this? [Re: azur] * 1
    #23841338 - 11/17/16 11:16 AM (7 years, 2 months ago)

Quote:

azur said:
I had an ape syringe that would not put out anything but bacteria. Finally I put it on antibiotic agar and sandwiched it with antibiotic agar. Clean my grew through and I very easily obtained a pure culture.



This...remember to switch to regular agar before going to gran though


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Invisibleazur
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Re: Grr.. what the hell is this? [Re: cronicr]
    #23841584 - 11/17/16 12:25 PM (7 years, 2 months ago)

Yup. Only use the antibiotic agar to obtain a pure culture. Only use it when battling bacteria, not mold. Once a pure culture is obtained, rocknroll with regular agar


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OfflineLogicaL ChaosM
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Re: Grr.. what the hell is this? [Re: cronicr]
    #23841586 - 11/17/16 12:26 PM (7 years, 2 months ago)

Quote:

cronicr said:
Quote:

azur said:
I had an ape syringe that would not put out anything but bacteria. Finally I put it on antibiotic agar and sandwiched it with antibiotic agar. Clean my grew through and I very easily obtained a pure culture.



This...remember to switch to regular agar before going to gran though




What happens if u dont switch?


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Invisibleazur
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Re: Grr.. what the hell is this? [Re: LogicaL Chaos] * 1
    #23841592 - 11/17/16 12:27 PM (7 years, 2 months ago)

You're wasting money.


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OfflinecronicrFacebook
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Re: Grr.. what the hell is this? [Re: LogicaL Chaos] * 1
    #23841599 - 11/17/16 12:30 PM (7 years, 2 months ago)

Quote:

LogicaL Chaos said:
Quote:

cronicr said:
Quote:

azur said:
I had an ape syringe that would not put out anything but bacteria. Finally I put it on antibiotic agar and sandwiched it with antibiotic agar. Clean my grew through and I very easily obtained a pure culture.



This...remember to switch to regular agar before going to gran though




What happens if u dont switch?



The antibiotis don't kill bacterIA they just stop it so when you go to grain it kicks in again.


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Offlinespacechildo
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Re: Grr.. what the hell is this? [Re: azur]
    #23841602 - 11/17/16 12:32 PM (7 years, 2 months ago)

and you wont see if your transfers are clean of the bacteria since the bac wouldn't grow on antibac-agar. it would once you put it to grain tho.

edit cron you fuckin type-nerd..  :stoner:


Edited by spacechildo (11/17/16 12:32 PM)


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OfflineLogicaL ChaosM
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Re: Grr.. what the hell is this? [Re: cronicr]
    #23841611 - 11/17/16 12:34 PM (7 years, 2 months ago)

How intriguring.

Interesting....


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OfflineTaizzen
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Re: Grr.. what the hell is this? [Re: LogicaL Chaos]
    #23841784 - 11/17/16 01:34 PM (7 years, 2 months ago)

Levster:
Pour and sterilize agar, then under your SAB, just open 1 plats like you were going to inoculate but don't !
Close and wait 1 week to see if its agar stays clean.
if you see pink color you'll know that it is not the serynge.
When you new to agar, It is a good idea to have a "witness" agar plate every time you work under your "sterile" setup until you perfect your technic.


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OfflineKenetic
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Re: Grr.. what the hell is this? [Re: Taizzen]
    #23842007 - 11/17/16 02:35 PM (7 years, 2 months ago)

I would also like to add that you can do blank transfers as well to see if you get any contams from your technique.  I'm confident that the agar plate full of bacteria in your post is from the inoculant though.


--------------------
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OfflineTaizzen
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Re: Grr.. what the hell is this? [Re: Kenetic]
    #23842556 - 11/17/16 04:53 PM (7 years, 2 months ago)

Quote:

kenetic said:
I would also like to add that you can do blank transfers as well to see if you get any contams from your technique.  I'm confident that the agar plate full of bacteria in your post is from the inoculant though.




yeah ! ! blank transfert ! ! :laugh:


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OfflineLevster
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Re: Grr.. what the hell is this? [Re: crackbaby]
    #23870413 - 11/26/16 08:30 PM (7 years, 2 months ago)

edit -- nvm


Edited by Levster (11/26/16 11:33 PM)


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