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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,360
Loc: The Inexpressible...
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Re: Grr.. what the hell is this? [Re: azur]
#23840652 - 11/17/16 03:48 AM (7 years, 2 months ago) |
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Quote:
azur said: Bacteria. Try antibiotic agar
This is real?!
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth πππ
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The Mycologist
Explorer

Registered: 05/06/16
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Yeah they use aquarium antibiotics. But it seems a lot of people don't like it.
-------------------- "That you are hereβthat life exists, and identity; That the powerful play goes on, and you will contribute a verse.β β Walt Whitman, Leaves of Grass

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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,360
Loc: The Inexpressible...
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Best to not use antibiotic agar huh? I could see that.
I would argue that being super sterile around and with regular agar to prevent infections.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Not to disagree with Azur, but my thought is if your agar is so bad you need antibiotic agar, you might as well just start over. Sorry to be blunt, but you obviously weren't trying hard enough.
-------------------- KSSS And PE WBS.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Grr.. what the hell is this? [Re: Moabfighter] 3
#23841129 - 11/17/16 10:07 AM (7 years, 2 months ago) |
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What azur meant was if his spore source is bacterial, use antibacterial agar, and if op did things as right as he says, the bacteria is undoubtedly because of the syringe.
There's many people I know (kizzle especially swears by this method) who either germs or transfers to antibacterial agar at least once in case there's any hidden bacteria growing with the mycelium.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Grr.. what the hell is this? [Re: Mad Season]
#23841132 - 11/17/16 10:08 AM (7 years, 2 months ago) |
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Alright I apologize for bad information then. Disregard. Sorry.
-------------------- KSSS And PE WBS.
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crackbaby
shitpost aficionado



Registered: 08/31/15
Posts: 12,994
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Re: Grr.. what the hell is this? [Re: Moabfighter]
#23841158 - 11/17/16 10:17 AM (7 years, 2 months ago) |
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i've gotten plenty of dud syringes, which is what seems to be the case here. It took a while racking my brain trying to figure out what i was doing wrong before finally realizing that half the syringes i was getting from a certain vendor were dead. Once i switched to a reliable vendor it was smooth sailing. I highly recommend sporeworks.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Grr.. what the hell is this? [Re: crackbaby]
#23841176 - 11/17/16 10:24 AM (7 years, 2 months ago) |
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I had an ape syringe that would not put out anything but bacteria. Finally I put it on antibiotic agar and sandwiched it with antibiotic agar. Clean my grew through and I very easily obtained a pure culture.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,360
Loc: The Inexpressible...
Last seen: 3 hours, 7 minutes
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Re: Grr.. what the hell is this? [Re: Mad Season]
#23841294 - 11/17/16 11:05 AM (7 years, 2 months ago) |
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Quote:
Mad Season said: What azur meant was if his spore source is bacterial, use antibacterial agar, and if op did things as right as he says, the bacteria is undoubtedly because of the syringe.
There's many people I know (kizzle especially swears by this method) who either germs or transfers to antibacterial agar at least once in case there's any hidden bacteria growing with the mycelium.
Im dumbfounded vendors even sell baterial syringes. blows my mind.
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Grr.. what the hell is this? [Re: azur] 1
#23841338 - 11/17/16 11:16 AM (7 years, 2 months ago) |
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Quote:
azur said: I had an ape syringe that would not put out anything but bacteria. Finally I put it on antibiotic agar and sandwiched it with antibiotic agar. Clean my grew through and I very easily obtained a pure culture.
This...remember to switch to regular agar before going to gran though
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Grr.. what the hell is this? [Re: cronicr]
#23841584 - 11/17/16 12:25 PM (7 years, 2 months ago) |
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Yup. Only use the antibiotic agar to obtain a pure culture. Only use it when battling bacteria, not mold. Once a pure culture is obtained, rocknroll with regular agar
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Re: Grr.. what the hell is this? [Re: cronicr]
#23841586 - 11/17/16 12:26 PM (7 years, 2 months ago) |
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Quote:
cronicr said:
Quote:
azur said: I had an ape syringe that would not put out anything but bacteria. Finally I put it on antibiotic agar and sandwiched it with antibiotic agar. Clean my grew through and I very easily obtained a pure culture.
This...remember to switch to regular agar before going to gran though
What happens if u dont switch?
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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You're wasting money.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Quote:
LogicaL Chaos said:
Quote:
cronicr said:
Quote:
azur said: I had an ape syringe that would not put out anything but bacteria. Finally I put it on antibiotic agar and sandwiched it with antibiotic agar. Clean my grew through and I very easily obtained a pure culture.
This...remember to switch to regular agar before going to gran though
What happens if u dont switch?
The antibiotis don't kill bacterIA they just stop it so when you go to grain it kicks in again.
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Grr.. what the hell is this? [Re: azur]
#23841602 - 11/17/16 12:32 PM (7 years, 2 months ago) |
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and you wont see if your transfers are clean of the bacteria since the bac wouldn't grow on antibac-agar. it would once you put it to grain tho.
edit cron you fuckin type-nerd..
Edited by spacechildo (11/17/16 12:32 PM)
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,360
Loc: The Inexpressible...
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Re: Grr.. what the hell is this? [Re: cronicr]
#23841611 - 11/17/16 12:34 PM (7 years, 2 months ago) |
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How intriguring.
Interesting....
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Taizzen



Registered: 01/24/13
Posts: 133
Last seen: 5 years, 8 months
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Levster: Pour and sterilize agar, then under your SAB, just open 1 plats like you were going to inoculate but don't ! Close and wait 1 week to see if its agar stays clean. if you see pink color you'll know that it is not the serynge. When you new to agar, It is a good idea to have a "witness" agar plate every time you work under your "sterile" setup until you perfect your technic.
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Kenetic
Nam Sayin



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Re: Grr.. what the hell is this? [Re: Taizzen]
#23842007 - 11/17/16 02:35 PM (7 years, 2 months ago) |
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I would also like to add that you can do blank transfers as well to see if you get any contams from your technique. I'm confident that the agar plate full of bacteria in your post is from the inoculant though.
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Taizzen



Registered: 01/24/13
Posts: 133
Last seen: 5 years, 8 months
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Re: Grr.. what the hell is this? [Re: Kenetic]
#23842556 - 11/17/16 04:53 PM (7 years, 2 months ago) |
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Quote:
kenetic said: I would also like to add that you can do blank transfers as well to see if you get any contams from your technique. I'm confident that the agar plate full of bacteria in your post is from the inoculant though.
yeah ! ! blank transfert ! !
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Levster
Rice Rocketeer


Registered: 08/17/16
Posts: 272
Last seen: 28 days, 2 hours
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Re: Grr.. what the hell is this? [Re: crackbaby]
#23870413 - 11/26/16 08:30 PM (7 years, 2 months ago) |
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edit -- nvm
Edited by Levster (11/26/16 11:33 PM)
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