Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom   MagicBag.co All-In-One Bags That Don't Suck   North Spore Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineRosen_Rot
Learning
I'm a teapot


Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 29 days
Agar taking too long to germinate spore?
    #23837820 - 11/16/16 06:32 AM (7 years, 3 months ago)

10/11/16 I inoculated 13 petri dishes with CFA, 10g cat food, 10g agar agar, 500ml water. Pced for 30mins after the rocker shakes, cooled down and then poured in front of a laminar starting from the bottom up

I followed standard procedure, burning the syringe needle till it's red hot on an alcohol lamp, pushing the plunger to cool down the needle and pushed the plunger again to drop 3 drops of B+ MS onto the centre of my agar mix

Its been 6 days and I haven't seen any mycelium development. There doesn't seem to be any mold growing on the agar either. Quite possibly some agar plates might have bacteria as I see on some sections of petris with a small uprised agar bubble.

They are marked with a red circle.

Some of the petris have like powder on the outside of the petri lid but that is okay cause it's just from my powdered surgical gloves. Some of my petris also have condensation so it might look milky. I assure you on the inside they look like it's just agar.

The syringe was a bit old, I bought it last year and stored it in the fridge where it stayed at 4C all summer

How long does it usually take on agar for spores to germinate??

Did I over load the agar dishes with spores and is that why they won't germinate??

Should I throw them away?? :sad:



--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
''there is no loneliness, only moments where contentment is fleeting''

SBJs "The Basics"
3iRiS9 "Cirque du freak"
B+ BONANZA

   

Extras: Filter Print Post Top
Offlineenlightenment
alchemist
 User Gallery

Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
Re: Agar taking too long to germinate spore? [Re: Rosen_Rot]
    #23837825 - 11/16/16 06:38 AM (7 years, 3 months ago)

Wait a few more days. Old spores can take more time to germinate IME. I had massive problems with old spore prints. I made syringes out of the old prints and it helped me a lot (hydration). But you already have a syringe. You can try to inoculate BRF paste.

Extras: Filter Print Post Top
OfflineRosen_Rot
Learning
I'm a teapot


Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 29 days
Re: Agar taking too long to germinate spore? [Re: enlightenment]
    #23837831 - 11/16/16 06:41 AM (7 years, 3 months ago)

I inoculated more petris this morning with a KSSS swab and also waiting for some agar to finish curing before I make more plates with whatever is left in the B+ syringe.

Luckily I got some grain jars so if all else fails I can always introduce a colonized grain.

It just sucks when wasting so much dishes and time

BTW

Agar plates are being stored at 21-22C


--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
''there is no loneliness, only moments where contentment is fleeting''

SBJs "The Basics"
3iRiS9 "Cirque du freak"
B+ BONANZA

   

Edited by Rosen_Rot (11/16/16 06:41 AM)

Extras: Filter Print Post Top
Offlineenlightenment
alchemist
 User Gallery

Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
Re: Agar taking too long to germinate spore? [Re: enlightenment]
    #23837842 - 11/16/16 06:51 AM (7 years, 3 months ago)

That would have been my next question... temperature is fine.
I know the pain.. Tossed 20 dishes after trying to get growth from a Psi. mexicana A print.

Some of the dishes look like they show signs of growth IMO.
It often starts to look milky where you inoculated. Can you make single pictures?

Especially the dishes you marked with red circles look like they started to germinate.

Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: Agar taking too long to germinate spore? [Re: enlightenment]
    #23837857 - 11/16/16 07:05 AM (7 years, 3 months ago)

Why CFA?  Try a different agar recipe.  This is the first I've seen anyone using CFA.  FH mentions it but I don't think it was his standard, more of an experiment it seemed.  Either way, you might try switching to something a little more in line with what is typically used...MEA, DFA, PDYA, etc.  cat food is super high in protein and fat.

Also, cubes typically germinate in 7 - 10 days. 


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

Edited by Mycolorado (11/16/16 07:11 AM)

Extras: Filter Print Post Top
OfflineRosen_Rot
Learning
I'm a teapot


Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 29 days
Re: Agar taking too long to germinate spore? [Re: Mycolorado]
    #23838346 - 11/16/16 10:28 AM (7 years, 3 months ago)

From my research, I believe it was a post by Pasty and a bunch of other agar recipe threads, CFA had some very interesting results and proved to be a very viable cheap source of media to cultivate mycelium. For me it was the easiest to acquire to my circumstance, my mother keeps cats so we get high quality cat food like WHISKAS..etc

I tried looking for LME and ME but couldn't find a good decently priced source. All online sources proved to be very expensive or just not the right stuff I was looking for.

I will be trying DFA, any recommendation as to brand?

Enlightenment; I wasn't sure if the milky streaks within the plates were mycelium development, condensation or mold from taking too long to germinate. Glad to know that they might be showing signs of life, that's a step in the right direction :thumbup:

I will try and get individual pictures tomorrow evening so look out for this thread

And thank you to anyone responding to this thread, I appreciate the help :shineon:


--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
''there is no loneliness, only moments where contentment is fleeting''

SBJs "The Basics"
3iRiS9 "Cirque du freak"
B+ BONANZA

   

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Agar taking too long to germinate spore? [Re: Mycolorado]
    #23838408 - 11/16/16 10:51 AM (7 years, 3 months ago)

Quote:

Mycolorado said:
Why CFA?  Try a different agar recipe.  This is the first I've seen anyone using CFA.  FH mentions it but I don't think it was his standard, more of an experiment it seemed.  Either way, you might try switching to something a little more in line with what is typically used...MEA, DFA, PDYA, etc.  cat food is super high in protein and fat.

Also, cubes typically germinate in 7 - 10 days. 



Do a search on cat and dog food agar. They were the rage here a year or 2 ago


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: Agar taking too long to germinate spore? [Re: Mad Season]
    #23838428 - 11/16/16 10:59 AM (7 years, 3 months ago)

I use DFA all the time and suggested the OP try it.  What's that got to do with CFA being all the rage a couple years ago.  If it were good, it would still be the rage today.  But thanks for the useful tip.


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

Extras: Filter Print Post Top
OfflinePanarchist
Cyanescendant
 User Gallery


Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 8 months, 17 days
Re: Agar taking too long to germinate spore? [Re: Rosen_Rot]
    #23840046 - 11/16/16 08:56 PM (7 years, 3 months ago)

Quote:

Rosen_Rot said:

I tried looking for LME and ME but couldn't find a good decently priced source. All online sources proved to be very expensive or just not the right stuff I was looking for.

:shineon:




i got some Seaweed Solutions brand MEA off Ebay recently. it wasn't much more than the food grade agar. the food grade would have been $12.00 for 100 grams. the MEA was $16.95.


--------------------

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Agar taking too long to germinate spore? [Re: Panarchist] * 1
    #23840773 - 11/17/16 05:52 AM (7 years, 3 months ago)

Quote:

my mother keeps cats so we get high quality cat food like WHISKAS




DONT PASS ME NO REGULAR!!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 27 days, 13 hours
Re: Agar taking too long to germinate spore? [Re: blindingleaf]
    #23840799 - 11/17/16 06:21 AM (7 years, 3 months ago)

You only need one drop of spores solution, there are so many spores in even a clear section of the sryinge. They should show growth soon.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

Extras: Filter Print Post Top
InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Agar taking too long to germinate spore? [Re: The Mycologist]
    #23840831 - 11/17/16 06:52 AM (7 years, 3 months ago)

I feel your pain. Been trying to get spores to germinate on agar for a couple months now :lol:
I just recently put them in a syringe and dropped on both agar and BRF paste which I hear is a good way to germ before moving to agar.

My AA+ spores on the other hand took ~2 weeks to germ. Just be patient for now, I wouldn't rule them out as duds just yet!


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself

Extras: Filter Print Post Top
OfflineRosen_Rot
Learning
I'm a teapot


Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 29 days
Re: Agar taking too long to germinate spore? [Re: ComebackKid]
    #23841869 - 11/17/16 01:58 PM (7 years, 3 months ago)

Hey guys sorry for the poor quality but I was in a hurry. Looks like I am starting to see whisps of mycelium starting to form and I think they are well and alive :shineon:

Quote:

ComebackKid said:
I feel your pain. Been trying to get spores to germinate on agar for a couple months now :lol:
I just recently put them in a syringe and dropped on both agar and BRF paste which I hear is a good way to germ before moving to agar.

My AA+ spores on the other hand took ~2 weeks to germ. Just be patient for now, I wouldn't rule them out as duds just yet!




I was under the impression that on agar spores would germinate within a few days :lol: I guess I'm still impatient but I think I am yielding some good results

Here are some pics





--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
''there is no loneliness, only moments where contentment is fleeting''

SBJs "The Basics"
3iRiS9 "Cirque du freak"
B+ BONANZA

   

Extras: Filter Print Post Top
InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Agar taking too long to germinate spore? [Re: Rosen_Rot]
    #23842874 - 11/17/16 06:07 PM (7 years, 3 months ago)

Congrats man! Yeah, I've read that spores can take up to 18 days on agar sometimes. If this hobby has taught me anything, it's a lesson in patients :lol:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself

Extras: Filter Print Post Top
Offlineenlightenment
alchemist
 User Gallery

Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
Re: Agar taking too long to germinate spore? [Re: ComebackKid]
    #23843904 - 11/18/16 12:26 AM (7 years, 3 months ago)

:thumbup:
Good that you did not toss them.
They already germinated on your first post. Those milky spots i wrote about. Good luck with your dishes and further projects

:goodluck:

Extras: Filter Print Post Top
OfflineRosen_Rot
Learning
I'm a teapot


Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 29 days
Re: Agar taking too long to germinate spore? [Re: enlightenment]
    #23843936 - 11/18/16 12:53 AM (7 years, 3 months ago)

:inlove3: :inlove3:

Super happy thank you so much for your input fellas really appreciate it!!

Unfortunately I wasn't so fortunate with the green elf cup fungus, only one plate left and seems inactive for now


--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
''there is no loneliness, only moments where contentment is fleeting''

SBJs "The Basics"
3iRiS9 "Cirque du freak"
B+ BONANZA

   

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom   MagicBag.co All-In-One Bags That Don't Suck   North Spore Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Re: Antibiotic Agar Mix for Spore Germiniation in Water? Anonymous 1,528 1 05/09/00 06:46 PM
by Anonymous
* germinating spores thescientist 1,569 5 10/04/02 12:53 PM
by thescientist
* how do u take a spore print? shroomer187 3,350 4 10/07/01 03:42 PM
by tchyted
* when to take the spore print ??veil wont brake lostagain 848 1 01/24/02 10:08 PM
by Azure
* Re: How do I use a sporeprint versus the normal spore syringe? Anonymous 2,320 11 07/26/00 09:54 AM
by psilocyber
* Whats with my agar plates? Frankenstein 8,670 8 09/08/22 03:46 PM
by schpat
* Re: agar questions Anonymous 1,409 6 05/30/00 03:07 PM
by Anonymous
* Agar question sill_o_cin 4,010 4 11/21/01 05:23 PM
by sill_o_cin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,060 topic views. 38 members, 166 guests and 42 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.004 seconds on 12 queries.