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Mattisfat
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King Oyster culture arrived
#23837710 - 11/16/16 04:45 AM (7 years, 2 months ago) |
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Thanks Ferather for sending me your culture.
Ive used your idea a bit and sterilised a toothpick in with my plates - il make sure transfers are done close to the toothpicks so if a contam occurs then I can make a quick, easy and clean transfer.
I will be growing these out onto straw eventually which I know isnt ideal for KO is there any supplements that can help with pasteurized straw?
Edited by Mattisfat (11/16/16 04:50 AM)
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Mattisfat
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Re: King Oyster culture arrived [Re: Mattisfat]
#23837718 - 11/16/16 04:51 AM (7 years, 2 months ago) |
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Btw the dirty looking stuff is where the agar has boiled up the side
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poofterFroth
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Re: King Oyster culture arrived [Re: Mattisfat]
#23837763 - 11/16/16 05:52 AM (7 years, 2 months ago) |
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Kings on pasteurized straw are fucking great. It's the method I use for most of my king oyster production and I'm always impressed. The only downside is sometime the mushrooms are almost to big.
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Ferather
Mycological



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Try a shallow jar. I got 4 x 250ml round containers from Wilko's for 99p. By the way that peg has a very strong isolate, very aggressive.
Enjoy your King oyster's, Ferather.
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23838096 - 11/16/16 08:45 AM (7 years, 2 months ago) |
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Quote:
Ferather said: Try a shallow jar. I got 4 x 250ml round containers from Wilko's for 99p. By the way that peg has a very strong isolate, very aggressive.
Enjoy your King oyster's, Ferather.
Thats cheap! Plastic or glass?
Ive not worked with plastic properly yet - apart from my melted test tube disaster! ive just been saving jam jars and sauce jars to use at the moment.
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Mattisfat
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Re: King Oyster culture arrived [Re: Mattisfat]
#23838125 - 11/16/16 08:57 AM (7 years, 2 months ago) |
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think i found them, look like the mini rounds that pastywhyte uses are they pp?
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23838136 - 11/16/16 09:03 AM (7 years, 2 months ago) |
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These ones, PP5 or above can be microwaved and pressure cooked.
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23838154 - 11/16/16 09:10 AM (7 years, 2 months ago) |
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Aye those are the ones i was looking at. I'll have to go grab some.
Love Wilkos like; their bags of WBS are dirt cheap.
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23838189 - 11/16/16 09:24 AM (7 years, 2 months ago) |
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They have tons of useful things, almost everything. Those pots you can mod with micro filter tape. Or keep them with lids on in the SAB, or another clean container with lid placed on.
There best seed mix is this one, it's premium and better quality.
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23838209 - 11/16/16 09:34 AM (7 years, 2 months ago) |
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That looks like what my Blue oyster spawn came on, i was thinking it was popcorn. Just got that in the post today as well so all stations go at the moment like. Im a cheap ass so if i can get the cheap stuff to be reliable then id rather struggle with prep haha
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Ferather
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Re: King Oyster culture arrived [Re: Mattisfat]
#23838222 - 11/16/16 09:41 AM (7 years, 2 months ago) |
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Do what you like, their cheap stuff you will need to make a small seed strainer. The peanuts and black sunflower seeds are not liked by mycelium.
Oyster's love most seeds, they are high in plant fiber.
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23838283 - 11/16/16 10:06 AM (7 years, 2 months ago) |
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I got one of the large wilko 50p buckets and hammered a ton of holes in the bottom for my strainer, to soak i just place that into a bucket with no holes. My plan is to go big....eventually. From watching the jars i have at the moment the myc happily just expands around the sunflower seeds - seems like a waste of time to get them out to me. I dunno i'll see how i feel after this bag runs out i may well switch to that budgee seed. Ideally i wanted rye or wheat grain but no where round here seems to sell it and shipping is expensive for large orders.
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Ferather
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Re: King Oyster culture arrived [Re: Mattisfat]
#23838326 - 11/16/16 10:22 AM (7 years, 2 months ago) |
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It seems the sunflower myth has already been busted, here. Just remove the peanuts it seems. Bucket is fine.
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Marty Mycfly
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Re: King Oyster culture arrived [Re: Ferather]
#23839579 - 11/16/16 05:29 PM (7 years, 2 months ago) |
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I'm looking forward to seeing this king culture you guys across the pond have.
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Mattisfat
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So the KO aggressively colonised the bottom of the peg but now it has reached the agar it looks like it is consolidating on the peg, is this normal?

Also slightly worried about the little dot of metabolites at the top, do you think i should try a transfer now or should i let it grow out on the agar a little?
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23853486 - 11/21/16 07:58 AM (7 years, 2 months ago) |
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The little drop of metabolites is in the area damaged by tools during transfer. Keep an eye on it. Oysters are wood lovers, so it will prefer the wood to the agar, it brings up nutrients.
So yes it is 100% normal behavior for a wood loving mycelium.
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Ferather
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Re: King Oyster culture arrived [Re: Ferather]
#23853522 - 11/21/16 08:20 AM (7 years, 2 months ago) |
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The other thing to note, although I know you are changing containers. The amount of air in that jar will take a while to build CO2.
When it does it will grow rapidly and push out.
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23853525 - 11/21/16 08:23 AM (7 years, 2 months ago) |
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I used tweezers which I dipped into a cap of iso right before I transferred. Think ill transfer to a new dish tommorow anyway - dont want to loose this to bacteria. never considered the co2 - I bought 4 packs of those wilko containers for my next batches
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Ferather
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Re: King Oyster culture arrived [Re: Mattisfat]
#23853561 - 11/21/16 08:36 AM (7 years, 2 months ago) |
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Ok fair enough, it is your decision. It could be secondary metabolites from repair. Usually they will get absorbed and used at a later date.
Good luck with your decision
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23853569 - 11/21/16 08:41 AM (7 years, 2 months ago) |
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Hmm now your making me wonder whether the transfer is needed - think il decide once I see how it reacts to the agar. I should know by tommorow.
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23853594 - 11/21/16 08:48 AM (7 years, 2 months ago) |
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If it gets bigger then transfer to T-Agar. But I doubt that will happen.
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23859470 - 11/23/16 04:17 AM (7 years, 2 months ago) |
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 so it looks like I have a white mold starting on the very edge of this jar - where is the healthiest looking section to transfer from? I cant tell if this is normal KO myc - looks totally different from the blue culture I have running.
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23859618 - 11/23/16 06:12 AM (7 years, 2 months ago) |
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It's not liking that starch agar at all, nor is that random mold. Cut the mold out, leave the KO to grow on the peg.
You could also remove the original peg.
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23859626 - 11/23/16 06:18 AM (7 years, 2 months ago) |
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I cant really cut it out as its climbed up the wall of the jar a little. Ive made a small transfer from behing the peg and will wait until the myc has colonised some of the tooth pick and transfer that aswell hopefully that will do.
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23859655 - 11/23/16 06:39 AM (7 years, 2 months ago) |
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A wood lover will prefer plant fiber over starch, so in this case the KO is bringing up nutrients and water to the wood. Your BO are from starch grain and you have transferred to starch agar, so it continues with its enzyme set.
Plant fiber is primary, anything else is secondary. You also have lots of O2 in that jar.
Why eat grass when you can eat a three course meal.
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MorePies
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Re: King Oyster culture arrived [Re: Ferather]
#23861745 - 11/23/16 07:32 PM (7 years, 2 months ago) |
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Looks like King myc to me. Kings love it all ferather. People see great yields on both Sawdust and straw etc. Mattisfat, you will probably find that the small transfer colonized very fast. Just like me on Sundays, mycelium isn't doing any work that it doesn't have to. If it has a lot or a complicated food to work on, it's not going to be in a hurry to move on.
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0yster
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Re: King Oyster culture arrived *DELETED* [Re: MorePies]
#23862837 - 11/24/16 07:50 AM (7 years, 2 months ago) |
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Post deleted by 0yster
Reason for deletion: -
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Mattisfat
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Re: King Oyster culture arrived [Re: 0yster]
#23862945 - 11/24/16 08:32 AM (7 years, 2 months ago) |
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Well id say contaminants got to the culture somehow - as im a noob id just blame me. Maybe i should have flame sterilised the tweezers instead of iso. The 'mold' at the sides is strangely slow making me think maybe its ps cyan spores that have germinated. Im going to properly clean my SAB out.
The myc however must be bacterial slightly. If my transfers arent great im going to try a tea agar.
Edited by Mattisfat (11/24/16 08:38 AM)
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23862982 - 11/24/16 08:52 AM (7 years, 2 months ago) |
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You are more honest that most. Still that is a shame. I can recommend a qualified vendor. That way you can buy clean spawn and start from there, learn agar on the side.
Here is one on the Shroomery vendor list, EU Vendor.
Bathed straw would be a good starter.
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MorePies
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Re: King Oyster culture arrived [Re: 0yster]
#23864454 - 11/24/16 07:25 PM (7 years, 2 months ago) |
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Quote:
0yster said: Explain this, here we have KO on starch free T-Gel.

Immediate transport to the birch peg. Mattisfat has one of the pegs.
What is your conclusion?
My observations have been such. When doing agar to agar transfers, there is a faster leap off from smaller wedges or just a light scraping of mycelium than from larger ones.
I would love to see a day by day sequence of photos documenting the growth of that "plate".
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Mattisfat
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Re: King Oyster culture arrived [Re: MorePies]
#23886854 - 12/02/16 08:26 AM (7 years, 1 month ago) |
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So the transfers i took came out perfect 

will be spawning these tonight or tommorow
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23886910 - 12/02/16 08:57 AM (7 years, 1 month ago) |
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Very well done, so your SAB was dirty, shame. Very nice transfer though, looks clean
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23886913 - 12/02/16 09:01 AM (7 years, 1 month ago) |
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Quote:
Ferather said: Very well done, so your SAB was dirty, booo. Very nice transfer though, looks clean 
To tell the truth i never bothered cleaning it haha
The contam turned out to be lots of bacteria in the end rather then spores. Still trying to figure out what went wrong but dont think ill ever know with that one.
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Mattisfat
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Re: King Oyster culture arrived [Re: Mattisfat]
#23886921 - 12/02/16 09:04 AM (7 years, 1 month ago) |
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When im spawning i was thinking of doing a tic tac toe pattern and making a blenderless LI, am i safe to assume the uncolonised agar is clean? or should i just use the myc
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23886927 - 12/02/16 09:08 AM (7 years, 1 month ago) |
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Much safer to use the colonized areas, what are you doing with the pegs? Transfer and re-grow?
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23886942 - 12/02/16 09:14 AM (7 years, 1 month ago) |
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Quote:
Ferather said: Much safer to use the colonized areas, what are you doing with the pegs? Transfer and re-grow?
Most likely - looks like its only reached the toothpick in one jar but that would do really. I'll use that transfer for long term storage and going to expand these spawn jars g2g and bulk it up.
Got these 1.25 litre jars at a TK Max (the ones with a home section called 'Homesense') 3 jars for £2. Will make great master jars and i can still fit in exactly the same amount in my PC.
Edit: sorry not 3 jars for £2 that would be amazing - it was 3 jars for £5
Edited by Mattisfat (12/02/16 09:20 AM)
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Ferather
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Re: King Oyster culture arrived [Re: Mattisfat]
#23886965 - 12/02/16 09:21 AM (7 years, 1 month ago) |
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Once it starts on a peg it will speed up and vitalize, agar is intentionally not rich. Those jars seem to be working, looks like there is enough CO2.
Try "The hedgehog" with lots of pegs, tea soaked.
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Mattisfat
Learning


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Re: King Oyster culture arrived [Re: Ferather]
#23886985 - 12/02/16 09:30 AM (7 years, 1 month ago) |
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I would say its more like stonehenge than a hedgehog.
.i might try sterilising some in a separate container one day when im making some agar - puts my mind at ease about hidden contams.
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23887000 - 12/02/16 09:37 AM (7 years, 1 month ago) |
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Gr0wer has made a great video on a few uses, here.
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Mattisfat
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Re: King Oyster culture arrived [Re: Ferather]
#23887011 - 12/02/16 09:44 AM (7 years, 1 month ago) |
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I like the look of that test tube idea - is it just sterile water with colonised agar dropped in? wondering how long that would last in storage. Think i would need a flow hood before i tried that one.
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23887015 - 12/02/16 09:47 AM (7 years, 1 month ago) |
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I would imagine, not 100% sure of Gr0wer's full setup. I would ask him, sorry.
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