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OfflineFerather
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Re: King Oyster culture arrived [Re: Mattisfat]
    #23853594 - 11/21/16 08:48 AM (7 years, 2 months ago)

If it gets bigger then transfer to T-Agar. But I doubt that will happen.


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OfflineMattisfat
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Re: King Oyster culture arrived [Re: Ferather]
    #23859470 - 11/23/16 04:17 AM (7 years, 2 months ago)


so it looks like I have a white mold starting on the very edge of this jar - where is the healthiest looking section to transfer from? I cant tell if this is normal KO myc - looks totally different from the blue culture I have running.


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OfflineFerather
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Re: King Oyster culture arrived [Re: Mattisfat]
    #23859618 - 11/23/16 06:12 AM (7 years, 2 months ago)

It's not liking that starch agar at all, nor is that random mold.
Cut the mold out, leave the KO to grow on the peg.

You could also remove the original peg.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineMattisfat
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Re: King Oyster culture arrived [Re: Ferather]
    #23859626 - 11/23/16 06:18 AM (7 years, 2 months ago)

I cant really cut it out as its climbed up the wall of the jar a little. Ive made a small transfer from behing the peg and will wait until the myc has colonised some of the tooth pick and transfer that aswell hopefully that will do.


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OfflineFerather
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Re: King Oyster culture arrived [Re: Mattisfat]
    #23859655 - 11/23/16 06:39 AM (7 years, 2 months ago)

A wood lover will prefer plant fiber over starch, so in this case the KO is bringing up nutrients and water to the wood.
Your BO are from starch grain and you have transferred to starch agar, so it continues with its enzyme set.

Plant fiber is primary, anything else is secondary. You also have lots of O2 in that jar.

Why eat grass when you can eat a three course meal.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineMorePies
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Re: King Oyster culture arrived [Re: Ferather]
    #23861745 - 11/23/16 07:32 PM (7 years, 2 months ago)

Looks like King myc to me. Kings love it all ferather. People see great yields on both Sawdust and straw etc. Mattisfat, you will probably find that the small transfer colonized very fast. Just like me on Sundays, mycelium isn't doing any work that it doesn't have to. If it has a lot or a complicated food to work on, it's not going to be in a hurry to move on.


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Offline0yster
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Re: King Oyster culture arrived *DELETED* [Re: MorePies]
    #23862837 - 11/24/16 07:50 AM (7 years, 2 months ago)

Post deleted by 0yster

Reason for deletion: -


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OfflineMattisfat
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Re: King Oyster culture arrived [Re: 0yster]
    #23862945 - 11/24/16 08:32 AM (7 years, 2 months ago)

Well id say contaminants got to the culture somehow - as im a noob id just blame me.
Maybe i should have flame sterilised the tweezers instead of iso. The 'mold' at the sides is strangely slow making me think maybe its ps cyan spores that have germinated. Im going to properly clean my SAB out.

The myc however must be bacterial slightly. If my transfers arent great im going to try a tea agar.


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Edited by Mattisfat (11/24/16 08:38 AM)


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OfflineFerather
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Re: King Oyster culture arrived [Re: Mattisfat]
    #23862982 - 11/24/16 08:52 AM (7 years, 2 months ago)

You are more honest that most. Still that is a shame. I can recommend a qualified vendor.
That way you can buy clean spawn and start from there, learn agar on the side.

Here is one on the Shroomery vendor list, EU Vendor.

Bathed straw would be a good starter.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineMorePies
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Re: King Oyster culture arrived [Re: 0yster]
    #23864454 - 11/24/16 07:25 PM (7 years, 2 months ago)

Quote:

0yster said:
Explain this, here we have KO on starch free T-Gel.

   

Immediate transport to the birch peg.
Mattisfat has one of the pegs.

What is your conclusion?




My observations have been such. When doing agar to agar transfers, there is a faster leap off from smaller wedges or just a light scraping of mycelium than from larger ones.

I would love to see a day by day sequence of photos documenting  the growth of that "plate".


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OfflineMattisfat
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Re: King Oyster culture arrived [Re: MorePies]
    #23886854 - 12/02/16 08:26 AM (7 years, 1 month ago)

So the transfers i took came out perfect :cool:



will be spawning these tonight or tommorow


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OfflineFerather
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Re: King Oyster culture arrived [Re: Mattisfat]
    #23886910 - 12/02/16 08:57 AM (7 years, 1 month ago)

Very well done, so your SAB was dirty, shame. Very nice transfer though, looks clean  :thumbup:


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineMattisfat
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Re: King Oyster culture arrived [Re: Ferather]
    #23886913 - 12/02/16 09:01 AM (7 years, 1 month ago)

Quote:

Ferather said:
Very well done, so your SAB was dirty, booo. Very nice transfer though, looks clean  :thumbup:



To tell the truth i never bothered cleaning it haha

The contam turned out to be lots of bacteria in the end rather then spores. Still trying to figure out what went wrong but dont think ill ever know with that one.


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OfflineMattisfat
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Re: King Oyster culture arrived [Re: Mattisfat]
    #23886921 - 12/02/16 09:04 AM (7 years, 1 month ago)

When im spawning i was thinking of doing a tic tac toe pattern and making a blenderless LI, am i safe to assume the uncolonised agar is clean? or should i just use the myc


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OfflineFerather
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Re: King Oyster culture arrived [Re: Mattisfat]
    #23886927 - 12/02/16 09:08 AM (7 years, 1 month ago)

Much safer to use the colonized areas, what are you doing with the pegs? Transfer and re-grow?


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineMattisfat
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Re: King Oyster culture arrived [Re: Ferather]
    #23886942 - 12/02/16 09:14 AM (7 years, 1 month ago)

Quote:

Ferather said:
Much safer to use the colonized areas, what are you doing with the pegs? Transfer and re-grow?



Most likely - looks like its only reached the toothpick in one jar but that would do really. I'll use that transfer for long term storage and going to expand these spawn jars g2g and bulk it up.

Got these 1.25 litre jars at a TK Max (the ones with a home section called 'Homesense') 3 jars for £2. Will make great master jars and i can still fit in exactly the same amount in my PC.

Edit: sorry not 3 jars for £2 that would be amazing - it was 3 jars for £5


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Edited by Mattisfat (12/02/16 09:20 AM)


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OfflineFerather
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Re: King Oyster culture arrived [Re: Mattisfat]
    #23886965 - 12/02/16 09:21 AM (7 years, 1 month ago)

Once it starts on a peg it will speed up and vitalize, agar is intentionally not rich.
Those jars seem to be working, looks like there is enough CO2.

Try "The hedgehog" with lots of pegs, tea soaked.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineMattisfat
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Re: King Oyster culture arrived [Re: Ferather]
    #23886985 - 12/02/16 09:30 AM (7 years, 1 month ago)

I would say its more like stonehenge than a hedgehog.

.i might try sterilising some in a separate container one day when im making some agar - puts my mind at ease about hidden contams.


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OfflineFerather
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Re: King Oyster culture arrived [Re: Mattisfat]
    #23887000 - 12/02/16 09:37 AM (7 years, 1 month ago)

Gr0wer has made a great video on a few uses, here.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineMattisfat
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Re: King Oyster culture arrived [Re: Ferather]
    #23887011 - 12/02/16 09:44 AM (7 years, 1 month ago)

I like the look of that test tube idea - is it just sterile water with colonised agar dropped in? wondering how long that would last in storage. Think i would need a flow hood before i tried that one.


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