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Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
Last seen: 3 years, 21 days
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King Oyster culture arrived
#23837710 - 11/16/16 04:45 AM (7 years, 2 months ago) |
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Thanks Ferather for sending me your culture.
Ive used your idea a bit and sterilised a toothpick in with my plates - il make sure transfers are done close to the toothpicks so if a contam occurs then I can make a quick, easy and clean transfer.
I will be growing these out onto straw eventually which I know isnt ideal for KO is there any supplements that can help with pasteurized straw?
Edited by Mattisfat (11/16/16 04:50 AM)
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Mattisfat
Learning


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Re: King Oyster culture arrived [Re: Mattisfat]
#23837718 - 11/16/16 04:51 AM (7 years, 2 months ago) |
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Btw the dirty looking stuff is where the agar has boiled up the side
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poofterFroth
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Re: King Oyster culture arrived [Re: Mattisfat]
#23837763 - 11/16/16 05:52 AM (7 years, 2 months ago) |
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Kings on pasteurized straw are fucking great. It's the method I use for most of my king oyster production and I'm always impressed. The only downside is sometime the mushrooms are almost to big.
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Ferather
Mycological



Registered: 03/19/15
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Try a shallow jar. I got 4 x 250ml round containers from Wilko's for 99p. By the way that peg has a very strong isolate, very aggressive.
Enjoy your King oyster's, Ferather.
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Mattisfat
Learning


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Posts: 570
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Re: King Oyster culture arrived [Re: Ferather]
#23838096 - 11/16/16 08:45 AM (7 years, 2 months ago) |
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Quote:
Ferather said: Try a shallow jar. I got 4 x 250ml round containers from Wilko's for 99p. By the way that peg has a very strong isolate, very aggressive.
Enjoy your King oyster's, Ferather.
Thats cheap! Plastic or glass?
Ive not worked with plastic properly yet - apart from my melted test tube disaster! ive just been saving jam jars and sauce jars to use at the moment.
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Mattisfat
Learning


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Posts: 570
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Re: King Oyster culture arrived [Re: Mattisfat]
#23838125 - 11/16/16 08:57 AM (7 years, 2 months ago) |
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think i found them, look like the mini rounds that pastywhyte uses are they pp?
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23838136 - 11/16/16 09:03 AM (7 years, 2 months ago) |
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These ones, PP5 or above can be microwaved and pressure cooked.
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Mattisfat
Learning


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Posts: 570
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Re: King Oyster culture arrived [Re: Ferather]
#23838154 - 11/16/16 09:10 AM (7 years, 2 months ago) |
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Aye those are the ones i was looking at. I'll have to go grab some.
Love Wilkos like; their bags of WBS are dirt cheap.
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23838189 - 11/16/16 09:24 AM (7 years, 2 months ago) |
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They have tons of useful things, almost everything. Those pots you can mod with micro filter tape. Or keep them with lids on in the SAB, or another clean container with lid placed on.
There best seed mix is this one, it's premium and better quality.
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Mattisfat
Learning


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Posts: 570
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Re: King Oyster culture arrived [Re: Ferather]
#23838209 - 11/16/16 09:34 AM (7 years, 2 months ago) |
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That looks like what my Blue oyster spawn came on, i was thinking it was popcorn. Just got that in the post today as well so all stations go at the moment like. Im a cheap ass so if i can get the cheap stuff to be reliable then id rather struggle with prep haha
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23838222 - 11/16/16 09:41 AM (7 years, 2 months ago) |
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Do what you like, their cheap stuff you will need to make a small seed strainer. The peanuts and black sunflower seeds are not liked by mycelium.
Oyster's love most seeds, they are high in plant fiber.
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Mattisfat
Learning


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Re: King Oyster culture arrived [Re: Ferather]
#23838283 - 11/16/16 10:06 AM (7 years, 2 months ago) |
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I got one of the large wilko 50p buckets and hammered a ton of holes in the bottom for my strainer, to soak i just place that into a bucket with no holes. My plan is to go big....eventually. From watching the jars i have at the moment the myc happily just expands around the sunflower seeds - seems like a waste of time to get them out to me. I dunno i'll see how i feel after this bag runs out i may well switch to that budgee seed. Ideally i wanted rye or wheat grain but no where round here seems to sell it and shipping is expensive for large orders.
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23838326 - 11/16/16 10:22 AM (7 years, 2 months ago) |
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It seems the sunflower myth has already been busted, here. Just remove the peanuts it seems. Bucket is fine.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
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Re: King Oyster culture arrived [Re: Ferather]
#23839579 - 11/16/16 05:29 PM (7 years, 2 months ago) |
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I'm looking forward to seeing this king culture you guys across the pond have.
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Mattisfat
Learning


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So the KO aggressively colonised the bottom of the peg but now it has reached the agar it looks like it is consolidating on the peg, is this normal?

Also slightly worried about the little dot of metabolites at the top, do you think i should try a transfer now or should i let it grow out on the agar a little?
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Ferather
Mycological



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Posts: 6,325
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Re: King Oyster culture arrived [Re: Mattisfat]
#23853486 - 11/21/16 07:58 AM (7 years, 2 months ago) |
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The little drop of metabolites is in the area damaged by tools during transfer. Keep an eye on it. Oysters are wood lovers, so it will prefer the wood to the agar, it brings up nutrients.
So yes it is 100% normal behavior for a wood loving mycelium.
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Ferather]
#23853522 - 11/21/16 08:20 AM (7 years, 2 months ago) |
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The other thing to note, although I know you are changing containers. The amount of air in that jar will take a while to build CO2.
When it does it will grow rapidly and push out.
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Mattisfat
Learning


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Re: King Oyster culture arrived [Re: Ferather]
#23853525 - 11/21/16 08:23 AM (7 years, 2 months ago) |
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I used tweezers which I dipped into a cap of iso right before I transferred. Think ill transfer to a new dish tommorow anyway - dont want to loose this to bacteria. never considered the co2 - I bought 4 packs of those wilko containers for my next batches
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Ferather
Mycological



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Re: King Oyster culture arrived [Re: Mattisfat]
#23853561 - 11/21/16 08:36 AM (7 years, 2 months ago) |
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Ok fair enough, it is your decision. It could be secondary metabolites from repair. Usually they will get absorbed and used at a later date.
Good luck with your decision
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Mattisfat
Learning


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Posts: 570
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Re: King Oyster culture arrived [Re: Ferather]
#23853569 - 11/21/16 08:41 AM (7 years, 2 months ago) |
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Hmm now your making me wonder whether the transfer is needed - think il decide once I see how it reacts to the agar. I should know by tommorow.
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