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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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can we stream sterilize our plastic plates making them no pour plates.
#23837395 - 11/15/16 11:58 PM (7 years, 2 months ago) |
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I have a crap ton of plastic cheap plates that arent in sleeves anymore. Would it be wrong to just mix the agar up heated up of course like most no pour recipes and then divide into those dishes. With the lids off let dry like 12 hours for no droplets. Then seal them up and stream on a rack for aleast a hour. Anything wrong with that?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23837416 - 11/16/16 12:07 AM (7 years, 2 months ago) |
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Doesn't your government grant cover shit like basic equipment?
Lol in all seriousness you will kill any mold that way but bacteria will be a different story. I won't say its 100% fail but certainly it's going to have a questionable success rate.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Pastywhyte]
#23837530 - 11/16/16 01:28 AM (7 years, 2 months ago) |
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I read a post recently that made me wonder the same thing. Someone suggested PCing the agar as normal, pouring the cleaned out petris, and then steam sterilizing them afterwards. As much of a pain in the ass it is to reuse plates, it would be a touch more ecofriendly while remaining low in cost.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23837578 - 11/16/16 02:23 AM (7 years, 2 months ago) |
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Maybe, but sounds kind of shabby. Try it tump, and let us know your results and if it don't work just toss them in a fire.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: wtfcrazymofo]
#23837594 - 11/16/16 02:51 AM (7 years, 2 months ago) |
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I will try after thanksgiving when i have more free time.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
#23842299 - 11/17/16 03:46 PM (7 years, 2 months ago) |
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Quote:
r.lutece said: I read a post recently that made me wonder the same thing. Someone suggested PCing the agar as normal, pouring the cleaned out petris, and then steam sterilizing them afterwards.
That was possibly me. I was speaking of PP plates, or glass, or other heat resistant dishes. But I am guessing the OP is using cheap PS polystyrene plates. These will deform very badly when steamed.
PP plates can stand up to pressure cooking, but they can degrade and deform and go cloudy after many cycles. My suggestion was to steam to prevent this happening as badly.
There are other methods to possibly sterilize the PS plates. I am going to be trying them again soon, using a bleach & vinegar mixture. Some reckon it can be done with UVC light, which you do not want to play around with.
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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: blackout]
#23843656 - 11/17/16 10:11 PM (7 years, 2 months ago) |
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As i can see form this chart. Blackout is right the cheapest plates do poorly with wet and dry heat.https://www.industrialspec.com/resources/plastics-sterilization-compatibility
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23843703 - 11/17/16 10:33 PM (7 years, 2 months ago) |
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Seems like you could wash them in some kind of disinfectant soap bath, leave them submerged, and rinse them in a bath of sterilized water in front of your flowhood (if you have a hood). Then pour... Might work. Seems like there would be a lot of potential for problems though.
I have an idea for some quick and easy/cheap pourable petris that I am going to put to the test soon.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
Edited by Bigbadwooof (11/17/16 10:34 PM)
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: blackout]
#23843825 - 11/17/16 11:37 PM (7 years, 2 months ago) |
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Quote:
blackout said:
Quote:
r.lutece said: I read a post recently that made me wonder the same thing. Someone suggested PCing the agar as normal, pouring the cleaned out petris, and then steam sterilizing them afterwards.
That was possibly me. I was speaking of PP plates, or glass, or other heat resistant dishes. But I am guessing the OP is using cheap PS polystyrene plates. These will deform very badly when steamed.
PP plates can stand up to pressure cooking, but they can degrade and deform and go cloudy after many cycles. My suggestion was to steam to prevent this happening as badly.
There are other methods to possibly sterilize the PS plates. I am going to be trying them again soon, using a bleach & vinegar mixture. Some reckon it can be done with UVC light, which you do not want to play around with.
Gotcha, thank you for clarifying. I'll be keeping an eye out for the results for sure. Quick question: doesn't a vinegar/bleach solution put off chlorine gas?
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
#23844074 - 11/18/16 03:16 AM (7 years, 2 months ago) |
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Another thing you could do, is get a UV sterilizer... If you use a lot of plates, it will pay for itself... eventually lol! One of the sponsors sells them.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
#23844734 - 11/18/16 09:50 AM (7 years, 2 months ago) |
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Ive always read UV and even been told by people on this board that UV sterilizers are useless in this hobby
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 6 hours, 23 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: spore-ty]
#23847069 - 11/18/16 10:41 PM (7 years, 2 months ago) |
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Quote:
spore-ty said: Ive always read UV and even been told by people on this board that UV sterilizers are useless in this hobby
I'm pretty sure UV sterilizers are used to sterilize petri dishes in the first place, before you receive them. I think the shitty ones on amazon wouldn't do the trick though. You need something heavy duty. Something with a warning label on it, you know? I believe they can cause cancer.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
#23847335 - 11/19/16 12:58 AM (7 years, 2 months ago) |
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Plastic pre-sterile dishes are gamma irradiated not UV sterilized. UV turns polystyrene yellow and brittle unfortunately.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Greg]
#23847805 - 11/19/16 08:59 AM (7 years, 2 months ago) |
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From Experiments Using Non-Sterile Plates:
Quote:
Reusing Plastic Petri Plates. The standard dish is 100 x 15 mm. Most of them are used once and discarded. I save mine and try to figure ways to reuse them. Also in my plant micropropagation lab I use a special dish which is 100 mm dia and 50 mm tall and very expensive. Plastic dishes are made of polystyrene because it is crystal clear, but it crumples when autoclaved. The factory uses Cobalt-60 gamma radiation to sterilize them after they are packed in tough sealed platic bags. I sterilize them in 10% Chlorox (undiluted it is 5.25 % sodium hypochlorite). Make this by adding one volume of Chlorox to 9 parts tap water. This 10% Chlorox is used in homes, farms, and labs to sterilize many things, but it will corrode iron and many other metals. If leave the bleach on the plates, your experiments may be killed unless there is enough dilution. Therefore I dip them in sterile autoclaved water or rubbing alcohol which evaporates faster. However for most uses the wet Petri plates are fine. In my micropropagation lab, I fill the wet plates with sterile agar which was autoclaved in bottles or jars and plant my strawberry plants. Growth takes weeks, but very few dishes become contaminated. How long must you soak them to kill all the bacteria? Killing is a logarithmic function. You will never kill every organism in a million plates. I suggest you do a test. I let them soak 20 miuntes and I have no problems. Therefore less soaking would sterilize most plates. I handle them with sterile forceps. When not using the forceps, I stand them in 70% iso-propanol--bleach will corrode metals. You could use 70% iso-propanol to sterilze your plates, but bleach is cheaper. Of course I wash the plastic items before sterilizing them with 10% Chlorox.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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krypto2000
Unknown


Registered: 12/05/06
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
#23848301 - 11/19/16 11:40 AM (7 years, 2 months ago) |
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I've heard of people successfully using both UV and even isopropyl soaks in front of a flow hood. Is it worth the contam risk? I don't know. Isopopropyl isn't really any cheaper than buying new plates either. If you had a UV box I could definitely see throwing everything in a plastic bag and leaving it in there for a few hours. You'd have to plan ahead, but it's cheap and easy.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: krypto2000]
#23849939 - 11/19/16 09:18 PM (7 years, 2 months ago) |
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Can you buy a gamma irradiation setup for private use?
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Inocuole
Scalpel of Evil's Bane



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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof] 1
#23849944 - 11/19/16 09:20 PM (7 years, 2 months ago) |
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If so, can you put your dick in it?
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Inocuole]
#23849991 - 11/19/16 09:50 PM (7 years, 2 months ago) |
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I keep reading everywhere that trump has a government grant to grow mashrooms. Can someone link to the OP where he said that? Fucking missed it!!
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Inocuole]
#23852459 - 11/20/16 07:02 PM (7 years, 2 months ago) |
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Quote:
Inocuole said: If so, can you put your dick in it?
... I don't know, but I probably need to.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
#23852469 - 11/20/16 07:08 PM (7 years, 2 months ago) |
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Quote:
Bigbadwooof said:
Quote:
Inocuole said: If so, can you put your dick in it?
... I don't know, but I probably need to.
Yeah, women like gamma-sterilized dicks nowadays, not the smelly things most of you got.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Josex]
#23852695 - 11/20/16 08:51 PM (7 years, 2 months ago) |
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Quote:
Josex said:
Quote:
Bigbadwooof said:
Quote:
Inocuole said: If so, can you put your dick in it?
... I don't know, but I probably need to.
Yeah, women like gamma-sterilized dicks nowadays, not the smelly things most of you got.
How about gamma sterilized Penis Envy?
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Josex]
#23854429 - 11/21/16 02:13 PM (7 years, 2 months ago) |
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Quote:
Josex said: I keep reading everywhere that trump has a government grant to grow mashrooms. Can someone link to the OP where he said that? Fucking missed it!! 
utsf
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: wtfcrazymofo]
#23854439 - 11/21/16 02:19 PM (7 years, 2 months ago) |
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I did already mang, I was just hoping someone would spare me the gruelling effort.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Josex]
#23854482 - 11/21/16 02:35 PM (7 years, 2 months ago) |
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That is a common word posted by tump.
OP I wish I could whip up some clean old plastic plates, but If I wanted to save them I'd just buy the expensive glass ones.
It is like trying to reuse a plastic diaper or a cloth diaper.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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krypto2000
Unknown


Registered: 12/05/06
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: wtfcrazymofo]
#23854493 - 11/21/16 02:41 PM (7 years, 2 months ago) |
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Plastic diapers are reusable, just stick it under a UV lamp for a few hours.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 6 hours, 23 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: krypto2000]
#23854630 - 11/21/16 03:27 PM (7 years, 2 months ago) |
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Quote:
krypto2000 said: Plastic diapers are reusable, just stick it under a UV lamp for a few hours.
Nah, too much light. Put it next to your heat register for a month or so
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
Edited by Bigbadwooof (11/21/16 03:27 PM)
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 6 hours, 23 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
#23854634 - 11/21/16 03:28 PM (7 years, 2 months ago) |
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Quote:
r.lutece said: From Experiments Using Non-Sterile Plates:
Quote:
Reusing Plastic Petri Plates. The standard dish is 100 x 15 mm. Most of them are used once and discarded. I save mine and try to figure ways to reuse them. Also in my plant micropropagation lab I use a special dish which is 100 mm dia and 50 mm tall and very expensive. Plastic dishes are made of polystyrene because it is crystal clear, but it crumples when autoclaved. The factory uses Cobalt-60 gamma radiation to sterilize them after they are packed in tough sealed platic bags. I sterilize them in 10% Chlorox (undiluted it is 5.25 % sodium hypochlorite). Make this by adding one volume of Chlorox to 9 parts tap water. This 10% Chlorox is used in homes, farms, and labs to sterilize many things, but it will corrode iron and many other metals. If leave the bleach on the plates, your experiments may be killed unless there is enough dilution. Therefore I dip them in sterile autoclaved water or rubbing alcohol which evaporates faster. However for most uses the wet Petri plates are fine. In my micropropagation lab, I fill the wet plates with sterile agar which was autoclaved in bottles or jars and plant my strawberry plants. Growth takes weeks, but very few dishes become contaminated. How long must you soak them to kill all the bacteria? Killing is a logarithmic function. You will never kill every organism in a million plates. I suggest you do a test. I let them soak 20 miuntes and I have no problems. Therefore less soaking would sterilize most plates. I handle them with sterile forceps. When not using the forceps, I stand them in 70% iso-propanol--bleach will corrode metals. You could use 70% iso-propanol to sterilze your plates, but bleach is cheaper. Of course I wash the plastic items before sterilizing them with 10% Chlorox.
I say this is the best answer ^^^
I will be trying this. Although, I wouldn't do it without a hood.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
#23859710 - 11/23/16 07:17 AM (7 years, 2 months ago) |
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I think i found the answer. Just cleaning them out, pouring grain water agar into them and dry heat sterilize then at 170 for 2.5 hours while your grain pc. I did this five days ago with 8 of them and they haven't molded out yet. I stream some 6 days ago and all molded clusters this morning
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23859962 - 11/23/16 09:04 AM (7 years, 2 months ago) |
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Did you use the oven for dry sterilization?
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
#23860231 - 11/23/16 10:32 AM (7 years, 2 months ago) |
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Yes i did. Streaming cracks the lids. Im waiting a 30 days without contams before i can say reuseing with dry heat works.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23860438 - 11/23/16 12:14 PM (7 years, 2 months ago) |
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Quote:
tump said: Yes i did. Streaming cracks the lids. Im waiting a 30 days without contams before i can say reuseing with dry heat works.
I would imagine that dry heat may work for mold, but it wouldn't likely solve any bacterial problems. You may still get away with it regardless.
The other day I was pouring some plates in front of my hood, and I accidently nudged a stack of them just outside the flow. They hadn't been wrapped in parafilm yet, btw... All of them seem perfectly fine! I was a bit surprised.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23860554 - 11/23/16 12:58 PM (7 years, 2 months ago) |
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Quote:
r.lutece said: Quick question: doesn't a vinegar/bleach solution put off chlorine gas?
Yeah, it does, so you have to take precautions. Bleach is usually sold with sodium hydroxide, aka lye, aka caustic soda mixed in. This stabilizes it, the vinegar neutralizes it and makes it much more effective and also more unstable, which is what I actually want. It is commonly used by homebrew people to make a "no rinse sanitiser", i.e. they wash bottles out with it, leave them upside down to drain and do not bother rinsing anymore, they pour beer right into them. I would use a stronger concentration than them.
I tried it before and dried them out on a heat pad, however I was very haphazard doing it all so most did fail but I still think it is worth trying again. Some are still OK many months on, I just left them be.
Quote:
Bigbadwooof said: rinse them in a bath of sterilized water in front of your flowhood (if you have a hood).
Rather than an actual bath I was thinking of a bottle of sterile water, as an actual bath would be hard to work with as it would be easily contaminated with dipping stuff in. I have PP plastic bottles that had ketchup in them, they have squirty lids. I can easily sterilize water in them and then rinse out the petris. However this may be unnecessary as if I just leave them they will degrade as the bleach vinegar solution is unstable. They could also be opened and flicked with a finger to try and get droplets off. If some remain on the lid they will hopefully just stay there, and slowly degrade.
I would prefer them to be dry, I could put them on a lower power heat pad or I was thinking of putting them in my drying chamber with desiccant for a few days or a week. This may not be needed, if there is liquid still in them it will hopefully have dropped off the lids. Even if it is still has active bleach in it when you pour in agar it might seal it in down the bottom, you might have to pour agar in, let it set, and then pour another layer on top, the free liquid in the bottom should mix with the first bit of agar poured in. People do bleach dunks with cakes, so for all I know a bit of residual bleach might be beneficial.
Quote:
tump said: I think i found the answer. Just cleaning them out, pouring grain water agar into them and dry heat sterilize then at 170 for 2.5 hours while your grain pc.
did they appear to dry out at all? if none contaminate I would take the lid off one as a control to purposely contaminate it and be sure that it will support growth.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: blackout]
#23861913 - 11/23/16 08:54 PM (7 years, 2 months ago) |
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Quote:
blackout said:
Quote:
tump said: I think i found the answer. Just cleaning them out, pouring grain water agar into them and dry heat sterilize then at 170 for 2.5 hours while your grain pc.
did they appear to dry out at all? if none contaminate I would take the lid off one as a control to purposely contaminate it and be sure that it will support growth.
I think he might have been just sterilizing empty plates with the intention of pouring later. However, if you could do no-pour in the oven, that's pretty sweet.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Grundalizer
Stranger

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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
#23861953 - 11/23/16 09:08 PM (7 years, 2 months ago) |
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Spray them with 70% ethanol and let dry in front of laminar flow hood, or sterilize under UV light. Sterilize agar media as normal via PC. You're good to go.
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tump
ban the undead


Registered: 03/17/16
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Grundalizer]
#23861963 - 11/23/16 09:11 PM (7 years, 2 months ago) |
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I did no pour in the oven. The agar doesn't look dry but i could test one by opening it.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23861983 - 11/23/16 09:23 PM (7 years, 2 months ago) |
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Quote:
tump said: I did no pour in the oven. The agar doesn't look dry but i could test one by opening it.

Went back and reread the post. I guess it would help if I had used my eyes the first time. :\
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
Edited by r.lutece (11/23/16 09:32 PM)
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blackout


Registered: 07/16/00
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23863792 - 11/24/16 02:16 PM (7 years, 2 months ago) |
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Quote:
r.lutece said: Went back and reread the post. I guess it would help if I had used my eyes the first time. :\
In fairness I had to read it a few times myself too. And still not sure what this meant.
Quote:
tump said: I think i found the answer. Just cleaning them out, pouring grain water agar into them and dry heat sterilize then at 170 for 2.5 hours while your grain pc.
I can only guess tump means he did this while PCing grains at the same time, but sounded like it was part of the process.
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Spore Ninja
PsychoMycoPhile



Registered: 10/25/16
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
#23864185 - 11/24/16 05:47 PM (7 years, 2 months ago) |
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Quote:
Bigbadwooof said:
Quote:
Inocuole said: If so, can you put your dick in it?
... I don't know, but I probably need to.
Warm agar and a Mickey's Big Mouth Bottle...
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Spore Ninja]
#23867828 - 11/26/16 02:55 AM (7 years, 2 months ago) |
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Quote:
Spore Ninja said:
Quote:
Bigbadwooof said:
Quote:
Inocuole said: If so, can you put your dick in it?
... I don't know, but I probably need to.
Warm agar and a Mickey's Big Mouth Bottle...
How warm is warm? Should I use gentamycin? You know what? Better safe than sorry.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 6 hours, 23 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
#23867830 - 11/26/16 02:59 AM (7 years, 2 months ago) |
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I actually think you could have a high success rate by just rinsing the insides of the petris out with a rubbing alcohol spray bottle in front of a hood. Spray til alcohol drips from the surface, and washes away any possible mold spores, then allow to air dry in front of the hood. It would likely kill all of the bacterial contaminants.
Could be hit and miss, but worth a shot. We are not doing the kind of sensitive lab work that requires 100% guaranteed sterile plates. It may save some money. Rubbing alcohol is cheap where I live ($2/bottle) , and I have to wait for plates in the mail also.
I will try this at some point and report back, if nobody else does.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
Edited by Bigbadwooof (11/26/16 03:00 AM)
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