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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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can we stream sterilize our plastic plates making them no pour plates.
#23837395 - 11/15/16 11:58 PM (7 years, 2 months ago) |
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I have a crap ton of plastic cheap plates that arent in sleeves anymore. Would it be wrong to just mix the agar up heated up of course like most no pour recipes and then divide into those dishes. With the lids off let dry like 12 hours for no droplets. Then seal them up and stream on a rack for aleast a hour. Anything wrong with that?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23837416 - 11/16/16 12:07 AM (7 years, 2 months ago) |
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Doesn't your government grant cover shit like basic equipment?
Lol in all seriousness you will kill any mold that way but bacteria will be a different story. I won't say its 100% fail but certainly it's going to have a questionable success rate.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Pastywhyte]
#23837530 - 11/16/16 01:28 AM (7 years, 2 months ago) |
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I read a post recently that made me wonder the same thing. Someone suggested PCing the agar as normal, pouring the cleaned out petris, and then steam sterilizing them afterwards. As much of a pain in the ass it is to reuse plates, it would be a touch more ecofriendly while remaining low in cost.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 14 hours, 19 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23837578 - 11/16/16 02:23 AM (7 years, 2 months ago) |
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Maybe, but sounds kind of shabby. Try it tump, and let us know your results and if it don't work just toss them in a fire.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: wtfcrazymofo]
#23837594 - 11/16/16 02:51 AM (7 years, 2 months ago) |
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I will try after thanksgiving when i have more free time.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
#23842299 - 11/17/16 03:46 PM (7 years, 2 months ago) |
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Quote:
r.lutece said: I read a post recently that made me wonder the same thing. Someone suggested PCing the agar as normal, pouring the cleaned out petris, and then steam sterilizing them afterwards.
That was possibly me. I was speaking of PP plates, or glass, or other heat resistant dishes. But I am guessing the OP is using cheap PS polystyrene plates. These will deform very badly when steamed.
PP plates can stand up to pressure cooking, but they can degrade and deform and go cloudy after many cycles. My suggestion was to steam to prevent this happening as badly.
There are other methods to possibly sterilize the PS plates. I am going to be trying them again soon, using a bleach & vinegar mixture. Some reckon it can be done with UVC light, which you do not want to play around with.
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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: blackout]
#23843656 - 11/17/16 10:11 PM (7 years, 2 months ago) |
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As i can see form this chart. Blackout is right the cheapest plates do poorly with wet and dry heat.https://www.industrialspec.com/resources/plastics-sterilization-compatibility
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 10 hours, 8 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: tump]
#23843703 - 11/17/16 10:33 PM (7 years, 2 months ago) |
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Seems like you could wash them in some kind of disinfectant soap bath, leave them submerged, and rinse them in a bath of sterilized water in front of your flowhood (if you have a hood). Then pour... Might work. Seems like there would be a lot of potential for problems though.
I have an idea for some quick and easy/cheap pourable petris that I am going to put to the test soon.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
Edited by Bigbadwooof (11/17/16 10:34 PM)
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: blackout]
#23843825 - 11/17/16 11:37 PM (7 years, 2 months ago) |
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Quote:
blackout said:
Quote:
r.lutece said: I read a post recently that made me wonder the same thing. Someone suggested PCing the agar as normal, pouring the cleaned out petris, and then steam sterilizing them afterwards.
That was possibly me. I was speaking of PP plates, or glass, or other heat resistant dishes. But I am guessing the OP is using cheap PS polystyrene plates. These will deform very badly when steamed.
PP plates can stand up to pressure cooking, but they can degrade and deform and go cloudy after many cycles. My suggestion was to steam to prevent this happening as badly.
There are other methods to possibly sterilize the PS plates. I am going to be trying them again soon, using a bleach & vinegar mixture. Some reckon it can be done with UVC light, which you do not want to play around with.
Gotcha, thank you for clarifying. I'll be keeping an eye out for the results for sure. Quick question: doesn't a vinegar/bleach solution put off chlorine gas?
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 10 hours, 8 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
#23844074 - 11/18/16 03:16 AM (7 years, 2 months ago) |
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Another thing you could do, is get a UV sterilizer... If you use a lot of plates, it will pay for itself... eventually lol! One of the sponsors sells them.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
#23844734 - 11/18/16 09:50 AM (7 years, 2 months ago) |
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Ive always read UV and even been told by people on this board that UV sterilizers are useless in this hobby
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 10 hours, 8 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: spore-ty]
#23847069 - 11/18/16 10:41 PM (7 years, 2 months ago) |
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Quote:
spore-ty said: Ive always read UV and even been told by people on this board that UV sterilizers are useless in this hobby
I'm pretty sure UV sterilizers are used to sterilize petri dishes in the first place, before you receive them. I think the shitty ones on amazon wouldn't do the trick though. You need something heavy duty. Something with a warning label on it, you know? I believe they can cause cancer.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
#23847335 - 11/19/16 12:58 AM (7 years, 2 months ago) |
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Plastic pre-sterile dishes are gamma irradiated not UV sterilized. UV turns polystyrene yellow and brittle unfortunately.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Greg]
#23847805 - 11/19/16 08:59 AM (7 years, 2 months ago) |
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From Experiments Using Non-Sterile Plates:
Quote:
Reusing Plastic Petri Plates. The standard dish is 100 x 15 mm. Most of them are used once and discarded. I save mine and try to figure ways to reuse them. Also in my plant micropropagation lab I use a special dish which is 100 mm dia and 50 mm tall and very expensive. Plastic dishes are made of polystyrene because it is crystal clear, but it crumples when autoclaved. The factory uses Cobalt-60 gamma radiation to sterilize them after they are packed in tough sealed platic bags. I sterilize them in 10% Chlorox (undiluted it is 5.25 % sodium hypochlorite). Make this by adding one volume of Chlorox to 9 parts tap water. This 10% Chlorox is used in homes, farms, and labs to sterilize many things, but it will corrode iron and many other metals. If leave the bleach on the plates, your experiments may be killed unless there is enough dilution. Therefore I dip them in sterile autoclaved water or rubbing alcohol which evaporates faster. However for most uses the wet Petri plates are fine. In my micropropagation lab, I fill the wet plates with sterile agar which was autoclaved in bottles or jars and plant my strawberry plants. Growth takes weeks, but very few dishes become contaminated. How long must you soak them to kill all the bacteria? Killing is a logarithmic function. You will never kill every organism in a million plates. I suggest you do a test. I let them soak 20 miuntes and I have no problems. Therefore less soaking would sterilize most plates. I handle them with sterile forceps. When not using the forceps, I stand them in 70% iso-propanol--bleach will corrode metals. You could use 70% iso-propanol to sterilze your plates, but bleach is cheaper. Of course I wash the plastic items before sterilizing them with 10% Chlorox.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: r.lutece]
#23848301 - 11/19/16 11:40 AM (7 years, 2 months ago) |
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I've heard of people successfully using both UV and even isopropyl soaks in front of a flow hood. Is it worth the contam risk? I don't know. Isopopropyl isn't really any cheaper than buying new plates either. If you had a UV box I could definitely see throwing everything in a plastic bag and leaving it in there for a few hours. You'd have to plan ahead, but it's cheap and easy.
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 10 hours, 8 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: krypto2000]
#23849939 - 11/19/16 09:18 PM (7 years, 2 months ago) |
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Can you buy a gamma irradiation setup for private use?
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof] 1
#23849944 - 11/19/16 09:20 PM (7 years, 2 months ago) |
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If so, can you put your dick in it?
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Inocuole]
#23849991 - 11/19/16 09:50 PM (7 years, 2 months ago) |
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I keep reading everywhere that trump has a government grant to grow mashrooms. Can someone link to the OP where he said that? Fucking missed it!!
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 10 hours, 8 minutes
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Inocuole]
#23852459 - 11/20/16 07:02 PM (7 years, 2 months ago) |
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Quote:
Inocuole said: If so, can you put your dick in it?
... I don't know, but I probably need to.
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: can we stream sterilize our plastic plates making them no pour plates. [Re: Bigbadwooof]
#23852469 - 11/20/16 07:08 PM (7 years, 2 months ago) |
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Quote:
Bigbadwooof said:
Quote:
Inocuole said: If so, can you put your dick in it?
... I don't know, but I probably need to.
Yeah, women like gamma-sterilized dicks nowadays, not the smelly things most of you got.
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