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Offlinefrankwhite85
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Close to monoculture? TC's only
    #23836917 - 11/15/16 08:58 PM (7 years, 2 months ago)

Here's one of my petri's from my latest round of cultures.  Started these from scratch from a B+ print, they are on their 10th transfer.  Trying to isolate to monocultures, are these close to defined sectoring or have I passed that point?



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OfflinePlazmotech
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Re: Close to monoculture? TC's only [Re: frankwhite85]
    #23836987 - 11/15/16 09:31 PM (7 years, 2 months ago)

Jesus Christ 10 transfers? I've never gotten that far so I'm not sure… is it really supposed to take that many? The teks I've read have said that it can take like… 3 transfers


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Offlinefrankwhite85
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Re: Close to monoculture? TC's only [Re: Plazmotech]
    #23836993 - 11/15/16 09:34 PM (7 years, 2 months ago)

3 is just if ur trying to get a clean culture to put to grain then hopefully get a clone.  I'm shooting for a mono I've read it takes at least 8-10 transfers to get defined sectoring then you sector out til u get isolates


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InvisibleBoogieman47
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Re: Close to monoculture? TC's only [Re: frankwhite85]
    #23837011 - 11/15/16 09:39 PM (7 years, 2 months ago)

Im not a tc but can tell you that you have alot of strains in those an isolate is uniform growth im sure you know that but see all the "fingers" it will be nowhere near that on an isolate ... basically if you took all the rizho away to the center there and that was all therr was that would be close to an isolate if you know what im sayin


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Re: Close to monoculture? TC's only [Re: Boogieman47]
    #23837049 - 11/15/16 09:58 PM (7 years, 2 months ago)

You are getting close to sectors. Should see some good ones soon. Couple more.


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Offlinefrankwhite85
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Re: Close to monoculture? TC's only [Re: Pastywhyte]
    #23837109 - 11/15/16 10:15 PM (7 years, 2 months ago)

So once each culture starts sectoring like the pic below I will have around 10-20 different sectors that I start isolating?



If so I need to start ordering a ton of petri's


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Re: Close to monoculture? TC's only [Re: frankwhite85]
    #23837115 - 11/15/16 10:18 PM (7 years, 2 months ago)

Yep. Even once sectors show it will take a while to work em all down.


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Offlinefrankwhite85
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Re: Close to monoculture? TC's only [Re: Pastywhyte]
    #23837186 - 11/15/16 10:35 PM (7 years, 2 months ago)

My god I know its gunna be lot of work but i think it'll be worth it to isolate at least one strain start to finish to become a buddha master of culturing haha.  I'll try to get some clones going in the mean time.


Edited by frankwhite85 (11/15/16 10:38 PM)


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Offlinewtfcrazymofo
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Re: Close to monoculture? TC's only [Re: frankwhite85]
    #23837639 - 11/16/16 03:33 AM (7 years, 2 months ago)

:thumbup:


--------------------
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Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




Edited by wtfcrazymofo (11/16/16 04:52 AM)


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InvisibleLocN9ne
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Re: Close to monoculture? TC's only [Re: wtfcrazymofo]
    #23837715 - 11/16/16 04:48 AM (7 years, 2 months ago)

You alright there mofo? Op those are looking good man... Try taking smaller transfers though, that seems like an awful lot of sectoring for 10 transfers deep.


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Offlinetripdawg420
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Re: Close to monoculture? TC's only [Re: LocN9ne]
    #23838069 - 11/16/16 08:34 AM (7 years, 2 months ago)

:congrats:


--------------------
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Invisibleazur
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Re: Close to monoculture? TC's only [Re: frankwhite85] * 1
    #23838124 - 11/16/16 08:57 AM (7 years, 2 months ago)

I'd love to offer my imput, but since it's tc's only, I can't.


--------------------


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Offlineenlightenment
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Re: Close to monoculture? TC's only [Re: azur] * 1
    #23838132 - 11/16/16 09:00 AM (7 years, 2 months ago)

Quote:

azur said:
I'd love to offer my imput, but since it's tc's only, I can't.



same here... :shrug:


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Invisibleazur
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Re: Close to monoculture? TC's only [Re: enlightenment] * 1
    #23838147 - 11/16/16 09:07 AM (7 years, 2 months ago)

Oh well


--------------------


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InvisiblebodhisattaMDiscordReddit
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Re: Close to monoculture? TC's only [Re: azur] * 1
    #23838175 - 11/16/16 09:19 AM (7 years, 2 months ago)

:whathesaid:
But I can still laugh at it


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Re: Close to monoculture? TC's only [Re: bodhisatta] * 1
    #23838183 - 11/16/16 09:22 AM (7 years, 2 months ago)

:highfive:


--------------------


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FALL IN LOVE WITH LC
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Re: Close to monoculture? TC's only [Re: azur]
    #23838196 - 11/16/16 09:29 AM (7 years, 2 months ago)

Hahahahahahahahaha wow op has won donkey of the day congrats mate... oh ya only two transfers in this b+ print I had and looks a lot closer to a mono than that..



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InvisibleBoogieman47
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Re: Close to monoculture? TC's only [Re: spore-ty]
    #23838203 - 11/16/16 09:32 AM (7 years, 2 months ago)

Dude i had shit that i swore was an isolate till i transferred again and bam like 40hundred sectors


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Offlinespore-ty
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Re: Close to monoculture? TC's only [Re: Boogieman47]
    #23838211 - 11/16/16 09:36 AM (7 years, 2 months ago)

Ive transferred 5 dofferent sections now from this plate all growth has been uniform making a tub soon


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InvisiblePastywhyteMDiscord
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Re: Close to monoculture? TC's only [Re: azur]
    #23838234 - 11/16/16 09:45 AM (7 years, 2 months ago)

Quote:

azur said:
I'd love to offer my imput, but since it's tc's only, I can't.




Don't sound salty azur, only reason you ain't a TC is cause you don't wanna be :shrug:


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InvisibleBoogieman47
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Re: Close to monoculture? TC's only [Re: spore-ty]
    #23838240 - 11/16/16 09:46 AM (7 years, 2 months ago)

Ill drop a plate that has tomentose and rhizo sectors haha check this guy out


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Offlinespore-ty
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Re: Close to monoculture? TC's only [Re: Pastywhyte]
    #23838248 - 11/16/16 09:48 AM (7 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

azur said:
I'd love to offer my imput, but since it's tc's only, I can't.




Don't sound salty azur, only reason you ain't a TC is cause you don't wanna be :shrug:





Wow did he decline?

No lie pasty and azur were the people I use to read all the time when I was a web crawler

@noob47 noice haha atleast its pinning


Edited by spore-ty (11/16/16 09:49 AM)


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InvisibleBoogieman47
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Re: Close to monoculture? TC's only [Re: spore-ty]
    #23838252 - 11/16/16 09:50 AM (7 years, 2 months ago)

You should of seen my ksss and mazzie plates i had canopies haha


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Offlinespore-ty
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Re: Close to monoculture? TC's only [Re: Boogieman47]
    #23838270 - 11/16/16 10:02 AM (7 years, 2 months ago)

Lol man im hoping i get some canopies...


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InvisibleBoogieman47
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Re: Close to monoculture? TC's only [Re: spore-ty]
    #23838276 - 11/16/16 10:04 AM (7 years, 2 months ago)

Not on agar you dont lmao


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Offlinespore-ty
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Re: Close to monoculture? TC's only [Re: Boogieman47]
    #23838310 - 11/16/16 10:14 AM (7 years, 2 months ago)

Lol true that, I really meant in my monos


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InvisibleBoogieman47
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Re: Close to monoculture? TC's only [Re: spore-ty]
    #23838319 - 11/16/16 10:18 AM (7 years, 2 months ago)

Haha ya i know im just messin i still have not got one in a mono im hoping soon


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Invisibleazur
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Re: Close to monoculture? TC's only [Re: Pastywhyte]
    #23838356 - 11/16/16 10:33 AM (7 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

azur said:
I'd love to offer my imput, but since it's tc's only, I can't.




Don't sound salty azur, only reason you ain't a TC is cause you don't wanna be :shrug:



I never said I don't want to be. I said I don't give a fuck!


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Offlinemrmazdarx9
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Re: Close to monoculture? TC's only [Re: azur]
    #23838364 - 11/16/16 10:35 AM (7 years, 2 months ago)

Quote:

azur said:
Quote:

Pastywhyte said:
Quote:

azur said:
I'd love to offer my imput, but since it's tc's only, I can't.




Don't sound salty azur, only reason you ain't a TC is cause you don't wanna be :shrug:



I never said I don't want to be. I said I don't give a fuck!



:lmao:


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InvisiblePastywhyteMDiscord
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Re: Close to monoculture? TC's only [Re: azur]
    #23838373 - 11/16/16 10:39 AM (7 years, 2 months ago)

Quote:

azur said:
Quote:

Pastywhyte said:
Quote:

azur said:
I'd love to offer my imput, but since it's tc's only, I can't.




Don't sound salty azur, only reason you ain't a TC is cause you don't wanna be :shrug:



I never said I don't want to be. I said I don't give a fuck!





:ohyou:


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Invisibleazur
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Re: Close to monoculture? TC's only [Re: Pastywhyte]
    #23838386 - 11/16/16 10:41 AM (7 years, 2 months ago)

Actually, it would be cool to get a tag so that when you create the congratulatory thread, I can simply respond with :splooge:


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleBoogieman47
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Re: Close to monoculture? TC's only [Re: azur]
    #23838418 - 11/16/16 10:56 AM (7 years, 2 months ago)

Lmao instant ban


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Offlinefrankwhite85
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Re: Close to monoculture? TC's only [Re: Boogieman47]
    #23838618 - 11/16/16 12:01 PM (7 years, 2 months ago)

I put TC's only because any time i put up an isolation question most people say you should have put that to grain already


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InvisiblePastywhyteMDiscord
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Re: Close to monoculture? TC's only [Re: frankwhite85]
    #23838662 - 11/16/16 12:13 PM (7 years, 2 months ago)

Quote:

frankwhite85 said:
I put TC's only because any time i put up an isolation question most people say you should have put that to grain already




To be honest isolation is a pain in the ass. Maybe you already have some good clones and done a bunch of ms grows so an isolation project isn't holding you back. But waiting on isolates to test before fruiting is also a waste IMO. That's probably why people say to just get it to grain.


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Offlinespacechildo
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Re: Close to monoculture? TC's only [Re: Pastywhyte]
    #23838686 - 11/16/16 12:19 PM (7 years, 2 months ago)

yeah, nothing wrong with taking 1 transfer to a new plate to isolate further and use the rest of the plate on some grains.
dont sit around :waitingpatiently: for an isolate with nothing else on your plate (pun not intented). not saying you are just saying if you are, then dont :super:


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Offlinefrankwhite85
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Re: Close to monoculture? TC's only [Re: Pastywhyte]
    #23838692 - 11/16/16 12:22 PM (7 years, 2 months ago)

I haven't had much luck with clones yet gotta keep trying.  Is it easier to just isolate from a clone? I saw this post and it seems like he was able to get mono's with just a few transfers.
https://www.shroomery.org/forums/showflat.php/Number/18219379/fpart/all/vc/1


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InvisibleBoogieman47
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Re: Close to monoculture? TC's only [Re: frankwhite85]
    #23838706 - 11/16/16 12:30 PM (7 years, 2 months ago)

Those were an ms clone fruit clones are a bit more reliable them ms and with a clone you dont have as many strains still alot but no where near ms if you dilute the spores its easier for an isolate


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Offlinefrankwhite85
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Re: Close to monoculture? TC's only [Re: Boogieman47]
    #23838718 - 11/16/16 12:37 PM (7 years, 2 months ago)

That's what I was thinking it narrows down way more then just from spores.  So I'm just going to keep on isolating but I'll take Spacechildo's advice and just start putting the rest of the plates to grain to work on some clones in the mean time.  When it comes to this hobby I think the more you can put into the pipeline to test the better haha


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InvisibleBoogieman47
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Re: Close to monoculture? TC's only [Re: frankwhite85]
    #23838722 - 11/16/16 12:40 PM (7 years, 2 months ago)

Thats it man ive been doing it for 8 months and havent even thought about an isolate after seeing all the vets here say how much of a chance youre taking on time and material for a crapshoot ... im not saying i wouldnt love a great isolate but its not on the to do list


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Re: Close to monoculture? TC's only [Re: Boogieman47]
    #23838740 - 11/16/16 12:49 PM (7 years, 2 months ago)

I spent a few months isolating a MS culture while fruiting MS tubs and clones. I finally just put my monoculture to the test and......slow colonization and small fruits regardless of conditions.

Here's a pic:




If this were my only project, and I waited months and months to fruit this- I might have quit the hobby! Lol

If you're isolating as a side-project, good on you. If it's your only project-be prepared for a slow colonizing, small fruit tub. If you are extremely lucky you'll get an awesome culture.
If your luck jar has run out-be prepared for a block of mycelium that won't even fruit....


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Offlinefrankwhite85
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Re: Close to monoculture? TC's only [Re: morty422]
    #23838762 - 11/16/16 12:58 PM (7 years, 2 months ago)

Was that the only mono you put to grain or was that the best performing out a group of mono's? either way that sucks ass


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Offlineenlightenment
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Re: Close to monoculture? TC's only [Re: frankwhite85]
    #23838773 - 11/16/16 01:03 PM (7 years, 2 months ago)

That's why a lot of people recommended to NOT make an isolate as your main project. And inoculate some grain with your plates. Sometimes people wait for an answer they want to hear. Isolates are crap shoots. You would have known that if you used the search function. I made about 30 isolates in my life and only 2 performed good enough to say it was worth the time and work.


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Re: Close to monoculture? TC's only [Re: morty422]
    #23838816 - 11/16/16 01:24 PM (7 years, 2 months ago)

Quote:

morty422 said:
I spent a few months isolating a MS culture while fruiting MS tubs and clones. I finally just put my monoculture to the test and......slow colonization and small fruits regardless of conditions.

Here's a pic:




If this were my only project, and I waited months and months to fruit this- I might have quit the hobby! Lol




:haha:


--------------------


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FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Offlinetombosley8
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Re: Close to monoculture? TC's only [Re: azur]
    #23839088 - 11/16/16 03:01 PM (7 years, 2 months ago)

Quote:

tombosley8 said:
I wouldn't worry about obtaining a monoculture .

Your better off fruiting a ms culture with slightly narrowed genetics after a transfer or two or three to clean it up and then taking some clones from those grows to agar for isolating certain traits but even then you won't have a mono culture most of the time.
just very narrowed genetics.

This will give you consistent results.

First fruiting from Ms(on agar) is a much easier way of obtaining better cultures than trying to obtain monocultures from MS because it gives you a good idea of what you are isolating and gives you a lot of variety to choose from.


I am sure there are situations where monocultures could be amazing but for someone just getting the feel of things it is way over the top IMO.




sorry I know this has already been said in a lot of ways but thought I'd add this as another way of saying the same thing...


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