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frankwhite85
Stranger


Registered: 04/18/16
Posts: 305
Last seen: 1 month, 6 days
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Re: Close to monoculture? TC's only [Re: morty422]
#23838762 - 11/16/16 12:58 PM (7 years, 2 months ago) |
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Was that the only mono you put to grain or was that the best performing out a group of mono's? either way that sucks ass
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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Re: Close to monoculture? TC's only [Re: frankwhite85]
#23838773 - 11/16/16 01:03 PM (7 years, 2 months ago) |
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That's why a lot of people recommended to NOT make an isolate as your main project. And inoculate some grain with your plates. Sometimes people wait for an answer they want to hear. Isolates are crap shoots. You would have known that if you used the search function. I made about 30 isolates in my life and only 2 performed good enough to say it was worth the time and work.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Close to monoculture? TC's only [Re: morty422]
#23838816 - 11/16/16 01:24 PM (7 years, 2 months ago) |
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Quote:
morty422 said: I spent a few months isolating a MS culture while fruiting MS tubs and clones. I finally just put my monoculture to the test and......slow colonization and small fruits regardless of conditions.
Here's a pic:

If this were my only project, and I waited months and months to fruit this- I might have quit the hobby! Lol
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 2 hours
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Re: Close to monoculture? TC's only [Re: azur]
#23839088 - 11/16/16 03:01 PM (7 years, 2 months ago) |
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Quote:
tombosley8 said: I wouldn't worry about obtaining a monoculture .
Your better off fruiting a ms culture with slightly narrowed genetics after a transfer or two or three to clean it up and then taking some clones from those grows to agar for isolating certain traits but even then you won't have a mono culture most of the time. just very narrowed genetics.
This will give you consistent results.
First fruiting from Ms(on agar) is a much easier way of obtaining better cultures than trying to obtain monocultures from MS because it gives you a good idea of what you are isolating and gives you a lot of variety to choose from.
I am sure there are situations where monocultures could be amazing but for someone just getting the feel of things it is way over the top IMO.
sorry I know this has already been said in a lot of ways but thought I'd add this as another way of saying the same thing...
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