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OfflineDabrit
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Re: Consensus on how many CC's of MS solution per quart jar of WBS? [Re: tombosley8]
    #23838658 - 11/16/16 12:11 PM (7 years, 2 months ago)

Quote:

tombosley8 said:
ditch the glove box and/or remove the gloves from the glove box and you have a SAB.
Spitball Jedi's SAB TEK

You should read this too!
Spiball Jedi's The Basics

Glove boxes make a ton of turbulence because of the piston effect. With six inch holes and no gloves attached there is very little turbulence when moving your hands in and out of the sab. Also you shouldn't be flame sterilizing inside the box as that creates currents from the heat blowing contams into your work.





When I say glove box it probably holds the same meaning as SAB. I don't have permanently attached gloves covering the holes into the box I just have to wholes cut in the side of it. I then just put on disposable rubber gloves and Tyvek coveralls cut off at the elbow with an elastic around my wrists and then enter into the box( After dipping them in 70% alcohol). And yeah I only flame sterilize outside of the box and then immediately but the syringe into the box for sterilization putting a couple of drops of ms onto the floor of the box to cool it down before inoculating.


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OfflineDabrit
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Re: Consensus on how many CC's of MS solution per quart jar of WBS? [Re: Mad Season]
    #23838671 - 11/16/16 12:15 PM (7 years, 2 months ago)

Quote:

Mad Season said:
Agar should technically be the same difficulty as the pf tek. Just 3 ingredients, mixed together at a ratio, just like the pf tek. Malt extract agar is my preferred choice, and that's 2g malt extract, 2g agar, and 100 ml of water.




I'm taking it this is the ratio to make just one petri dish worth of agar?

Coolio. I know the mixing part seems pretty elementary it's just the inoculating part and being able to decipher what is a clean culture and contaminates once I get going.

But I guess that's what this site is for a tool to learn and progress as a mycologist.


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Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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InvisibleMad Season
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Re: Consensus on how many CC's of MS solution per quart jar of WBS? [Re: Dabrit]
    #23838676 - 11/16/16 12:16 PM (7 years, 2 months ago)

Depends. That's more like 2-4 dishes. 60mm dishes use like 25 ml


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Offlinetombosley8
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Re: Consensus on how many CC's of MS solution per quart jar of WBS? [Re: Dabrit]
    #23839028 - 11/16/16 02:42 PM (7 years, 2 months ago)

Quote:

Dabrit said:
Quote:

tombosley8 said:

Spitball Jedi's SAB TEK

You should read this too!
Spiball Jedi's The Basics






When I say glove box it probably holds the same meaning as SAB. I don't have permanently attached gloves covering the holes into the box I just have to wholes cut in the side of it. I then just put on disposable rubber gloves and Tyvek coveralls cut off at the elbow with an elastic around my wrists and then enter into the box( After dipping them in 70% alcohol). And yeah I only flame sterilize outside of the box and then immediately but the syringe into the box for sterilization putting a couple of drops of ms onto the floor of the box to cool it down before inoculating.




Ok kool, sounds like you get the idea.

One more thing is make sure to wipe all jars and tools with iso and before getting to work mist down the inside box with soapy water being sure to saturate all walls ceiling and floor. Read the SAB tek I linked to and it explains it very well.


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Edited by tombosley8 (11/16/16 02:43 PM)


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OfflineDabrit
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Re: Consensus on how many CC's of MS solution per quart jar of WBS? [Re: tombosley8]
    #23839994 - 11/16/16 08:33 PM (7 years, 2 months ago)

Quote:

tombosley8 said:
Quote:

Dabrit said:
Quote:

tombosley8 said:

Spitball Jedi's SAB TEK

You should read this too!
Spiball Jedi's The Basics






When I say glove box it probably holds the same meaning as SAB. I don't have permanently attached gloves covering the holes into the box I just have to wholes cut in the side of it. I then just put on disposable rubber gloves and Tyvek coveralls cut off at the elbow with an elastic around my wrists and then enter into the box( After dipping them in 70% alcohol). And yeah I only flame sterilize outside of the box and then immediately but the syringe into the box for sterilization putting a couple of drops of ms onto the floor of the box to cool it down before inoculating.




Ok kool, sounds like you get the idea.

One more thing is make sure to wipe all jars and tools with iso and before getting to work mist down the inside box with soapy water being sure to saturate all walls ceiling and floor. Read the SAB tek I linked to and it explains it very well.




Yeah that's pretty much what I do. I clean the inside with iso,  I use a 1:10 bleach to water ratio though and mist the walls ceiling and floor after putting the jars inside. And mist in the air inside the box and let sit for 10 minutes.  I then iso my gloves and such and then remove the foil lids and then iso all sides of the jar and lids and then begin innoculating


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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Offlinetombosley8
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Re: Consensus on how many CC's of MS solution per quart jar of WBS? [Re: Dabrit]
    #23841270 - 11/17/16 10:57 AM (7 years, 2 months ago)

Nice vids on the subject

and I think the bleach is overkill... just irritates my lungs at best IMO


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Edited by tombosley8 (11/17/16 01:44 PM)


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OfflineGilfoil
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Registered: 01/31/17
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Re: Consensus on how many CC's of MS solution per quart jar of WBS? [Re: tombosley8]
    #25427467 - 08/31/18 11:32 PM (5 years, 4 months ago)

Hey everybody who contributed here. I found this incredibly helpful and just wanted to say thanks! bump


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Offlineentheo_heathen
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Re: Consensus on how many CC's of MS solution per quart jar of WBS? [Re: Gilfoil]
    #25427722 - 09/01/18 03:29 AM (5 years, 4 months ago)

:doublefacepalm:


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