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InvisibleComebackKid
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23839500 - 11/16/16 05:04 PM (7 years, 2 months ago)

I believe the steps you mentioned to be correct.
Make like 20ish plates up and put a single drop of solution in the center of maybe 6 plates after the agar solidifies.
You want to wrap your plates with cling film I believe to keep contams out.
Then wait for growth.
I'm not 100% certain as I hAve only ever fucked with no pour agar.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
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Our individual experiences are all part of the universe's experience of itself


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: ComebackKid]
    #23839671 - 11/16/16 06:10 PM (7 years, 2 months ago)

Once again, thanks for your time...

For someone who is familiar with pour agar, does this tek explain my steps properly for MAE after i mix the 25g - 500ml solution?


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OfflineThe Mycologist
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23839962 - 11/16/16 08:13 PM (7 years, 2 months ago)



--------------------
"That you are hereβ€”that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: The Mycologist]
    #23842402 - 11/17/16 04:16 PM (7 years, 2 months ago)

Thanks for all the help!whats the best option for sterilizing things that cant be flame sterilized, like SAB, peitris, spore syringe ect? Alcohol, bleach, peroxide or a combination?
Once i pour the PCd water/MEA, while it solidifies, what do i do to keep contams away before i noc it up? Just leave everything inside SAB till it hardens? Do i wait for room temp or what?


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23842445 - 11/17/16 04:25 PM (7 years, 2 months ago)

Also, ivalready started, at this time i am PC the 25g(scaled) to 500ml...
While i mixed the stuff in before shutting the lid, i got a spoon full of the WEA and let it cool outside the PC, so i had an idea how long it takes to harden. Well it is definitely room temp but the top is very watery while the bottom is a little gelly. Does it take time as well as temperature (cooling) to harden? I dont want to pour into my peitris and find out that it is too watery and i wasted my peitris... ugh this is frustrating? It is at or below room temp, and i digitally scalled to 25g and measured perfectly 500ml of water... is it just going to take time as well as cooling?


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23843038 - 11/17/16 06:48 PM (7 years, 2 months ago)

Any help with the watery situation? Been 2 hours,  probably ready to start pouring... does this shit dry over time? I weighed 25g and perfectly measured 500ml. The stuff i spooned out prior to PC just seems too watery on top, im afraid of pouring in dishes and ruining due to water content
It has been cool for almost 3 hours and it isnt actually watery,  it has the consistency of snot, not as watery on top, but it has long cooled


Edited by Shroomy_Mclovin (11/17/16 06:58 PM)


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InvisibleComebackKid
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23843078 - 11/17/16 06:59 PM (7 years, 2 months ago)

Yeah it will solidify once it cools down. It usually only takes 5 minutes or so for my no pour agar dishes to solidify after PC


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: ComebackKid]
    #23843155 - 11/17/16 07:22 PM (7 years, 2 months ago)

Then my mixture cant be right, this shit is actually cold at this point.... does the 25g x 500ml ratio seem correct?

Like i said i pulled this stuff out of the jar i PC right before i closed the lid.. and that was 3 hours ago. It is on a spoon sitting on my counter, the stuff is cold at this point yet still feels like snot


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Offlinetombosley8
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23843208 - 11/17/16 07:38 PM (7 years, 2 months ago)

Hey bud here is some good shit for agar and you should watch RR's vids lets grow mushroom series.
http://www.mushroomvideos.com/Agar-Petri-Dishes
also you should buy the vids they show the full videos and can really come in handy. Well worth it for all the info in them.

here are some more agar links and overall info for cleaning and isolating/cloning thanks to bodhisatta Agar links
all of this can be done in a SAB just as easy as long as it is tall enough or your agar bottle isn't too tall.

Did you get parafilm at the science store to wrap your petris after inoculation?


And spitball Jedi's stuff is awesome all around SBJ's The Basics


Edited by tombosley8 (11/17/16 09:15 PM)


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: tombosley8]
    #23844289 - 11/18/16 06:45 AM (7 years, 2 months ago)

Badass post! Yea i saw a lot of those, but thanks for the post regardless 100% greatful. Its just trippy because as im understanding, by purchasing this Malt Extract agar powder i bypassed all the legwork  of creating my own agar-mix. I have been researching a lot and I'm getting a better understanding as we all have, this is a big learning curve.
I feel like i have the idea, but what im stuck on right now is, why is my agar so runny and what  is my proper mix. I put the 25g x 500ml ratio in this batch of MAE and it is wayy too runny, i still have not opened my PC, but the test spoon is at the consistancy of strawberry-jelly.
The label calls for 28mg x 1L(1000ml) which means i needed an extra gram of powder. But i dont think it should have been so runny though.
Now im afraid to mess up another batch, i got the hook up from the science store because it was an order that was not picked up so the guy didnt want it to expire and sold me the $90 bottle for $30...


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23844438 - 11/18/16 07:56 AM (7 years, 2 months ago)

So i left it in the pc overnight and i finally opened the pc and took it out, knowing it was too cold to use but i was curious  of the consistancy. It did harden to what i believe is the proper thickness. However it is very moist. It is like a solid-gel consistancy, but it feels like i just ran it under water.... so if agar is on the same basis of BRF/WBS than it is certainly too wet but it is a good solid.. however if it is more forgiving with moisture, like a LC than it would have probably worked perfectly.maybe a couple more G of powder would fix the excess moisture?



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OfflineLogicaL ChaosM
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23844458 - 11/18/16 08:05 AM (7 years, 2 months ago)

Is that agar?

Or jelly popsicle?


--------------------
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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: LogicaL Chaos]
    #23844509 - 11/18/16 08:28 AM (7 years, 2 months ago)

Lol not a popsicle but it does look a lil yummy... i pcd it in a mason jar cas i didn't have a good jar for pouring, but that particular jar had an inoculation plug that i was going to pull out to pour once i was ready to fill my pietri dishes.. so yeah i pulled that directly from the mason jar, it was a somewhat firm gel but seemed too wet... is excess moisture an issue with MEA?


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Invisibler.lutece
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23844524 - 11/18/16 08:34 AM (7 years, 2 months ago)

That extra gram of powder might have been just the thing to soak up that extra moisture. It's a small amount, but just like in cooking, it can make a big difference.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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Re: Questionable mycelium [Re: r.lutece]
    #23845181 - 11/18/16 12:45 PM (7 years, 2 months ago)

Slightly wet is not an issue IME. I'd rather that than too dry.

I have pooling water and pretty soft agar in most of my dishes and It has caused no problems.

The thing with adding more or less powder is you are altering the nute content since yours is premixed.

usually with slightly less nutes I get faster growth and more rhizo growth Which I prefer and then switch back for the plate that I plan to inoculate grains/etc with.

I like making my own mix for that reason, so I'm not altering the agar content when adjusting the nutes.

You can order the ingredients really cheap off amazon or ebay.

Just look for Light Malt Extract and agar agar powder.  They are all pretty similar in consistency as I've used about 10 different brands.


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InvisibleComebackKid
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23845443 - 11/18/16 02:40 PM (7 years, 2 months ago)

Throw that back in the PC and pour your plates with it. Looks perfect:thumbup:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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