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OfflineShroomy_Mclovin
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Questionable mycelium
    #23835895 - 11/15/16 03:45 PM (7 years, 2 months ago)

I did 4 large jars and 2 halfpint jars of WBS and during PC i ran out of water after an hour and a half or so. Stupid mistake,  i know but after afew contaminated runs,  i wanted to sterilize good this one. so i took my chances and added hot water, and wound up cracking every jar, they didnt shadder but they definitely  cracked the bottoms real good. So i went and ran with it, didnt want to make more jars.
Next day i nocked them up with"creeper" spores from a syringe... well only one has any kind of growth, oddly it is the one with what i would consider too much moisture.
The other jars dont seem to have any mycelium, mold or bacteria, but this jar has questionable groth that looks more like cotton. Solid white fluffy growth. Not straky and not your typical  dense mycelium... any opinions? Thanks in advance


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InvisibleComebackKid
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23835899 - 11/15/16 03:46 PM (7 years, 2 months ago)

I didn't read your post yet but from what I can tell from pics you have mold:sad:

When I did my first PC run I ran out of water due to pcing at waaaaay to high of a temp. I did the same thing you did and cracked one of my jars.

I did a search and RR said that contaminants can still make it through the cracked glass.

I took my chances as well and wrapped cling film around the jar and taped it up with packing tape.
I ended up with clean spaw. Mind you, I was also inoculating with agar.

In your case I'm going to assume contamination came in through the cracks in your glass.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


Edited by ComebackKid (11/15/16 03:56 PM)


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OfflineTira
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Re: Questionable mycelium [Re: ComebackKid]
    #23836044 - 11/15/16 04:18 PM (7 years, 2 months ago)

That does not look like something you'd like to have in your jars.  Contam for sure.

Next time don't take any risks and start over when accidents happen.  It consumes more time to try fixing problems the accident causes, and mostly they can't be fixed.  You don't want to cut corners.  Doing a proper start is the way to go.


--------------------
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InvisibleComebackKid
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Re: Questionable mycelium [Re: Tira]
    #23836144 - 11/15/16 04:39 PM (7 years, 2 months ago)

:whathesaid:

Even though I still ended up with clean spawn it does NOT not rule out the luck factor by any means. I do not reccomend taping cling film to your jars, but rather reccomend PCing at a lowe temperature.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: ComebackKid]
    #23836428 - 11/15/16 06:18 PM (7 years, 2 months ago)

Lame-o.... well im screwed, i dont have any more syringes. I might pick up a kit from planet k to save some time(on shipping), then once i get some stong mycelium i will just expand into some clean PCed WBS...

Any experience with the PK kits?


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23838106 - 11/16/16 08:49 AM (7 years, 2 months ago)

Just remembered that i had 2(cc or mg i forget the measurement but it is filled to the 2 on the syringe) that was leftover from inoculation.
I just threw the syringe in a ziplock and stored it in my fridge, contams can definitely be an issue. But from  what i understand, peitri cultivation can be a good option because i can isolate good micelium. Any advice?
I also ready that potato dextrose can be a better option for tissue culture, while Malt dextrose would be better for spores... can anyone give me some 411 on the method i should look into?


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Offlinespore-ty
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23838210 - 11/16/16 09:34 AM (7 years, 2 months ago)

Look up pasty whyte agar tek I use extra light malt extract and agar for the most part i really reccommend petri dishes so you can see your mycelium

Also from time to time when working with the same culture in on going transfers I might switch up my agar recipe with pda or something but your gonna ahave to look into a pour agar tek which is the same thing as the pasty tek

Just pour your agar in a sab or in front of a fh is the difference really


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Offlinemrmazdarx9
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23838333 - 11/16/16 10:25 AM (7 years, 2 months ago)

Quote:

Shroomy_Mclovin said:
Lame-o.... well im screwed, i dont have any more syringes. I might pick up a kit from VENDOR to save some time(on shipping), then once i get some stong mycelium i will just expand into some clean PCed WBS...

Any experience with the VENDOR?



Dont post vendors :rules:


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: spore-ty]
    #23839109 - 11/16/16 03:07 PM (7 years, 2 months ago)

Quote:

spore-ty said:
Look up pasty whyte agar tek I use extra light malt extract and agar for the most part i really reccommend petri dishes so you can see your mycelium

Also from time to time when working with the same culture in on going transfers I might switch up my agar recipe with pda or something but your gonna ahave to look into a pour agar tek which is the same thing as the pasty tek

Just pour your agar in a sab or in front of a fh is the difference really




What kind of agar? Im outfront of the science store right now lol... gonna check out those teks you mentioned.. thanks so much for your assistance...


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OfflineThe Mycologist
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23839120 - 11/16/16 03:10 PM (7 years, 2 months ago)

It doesnt matter, Potato Dextrose/Malt Extract


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: mrmazdarx9]
    #23839121 - 11/16/16 03:10 PM (7 years, 2 months ago)

Quote:

mrmazdarx9 said:
Quote:

Shroomy_Mclovin said:
Lame-o.... well im screwed, i dont have any more syringes. I might pick up a kit from VENDOR to save some time(on shipping), then once i get some stong mycelium i will just expand into some clean PCed WBS...

Any experience with the VENDOR?



Dont post vendors :rules:




My bad did that absent mindedly bc i was thinking more on the lines of venders being spore/suppliers via internet.. rather than local shops, i see plenty of people recomending local suppliers and i never thought of it... however i thank you for your post which contributed NOTHING to my initial question.. and i say that with the upmost sarcasm


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: The Mycologist]
    #23839134 - 11/16/16 03:13 PM (7 years, 2 months ago)

Quote:

The Mycologist said:
It doesnt matter, Potato Dextrose/Malt Extract




Awesome... thanks... is there anything else that i MUST GET FROM SCIENCE store while im here or will these teks require things which i can get from regular stores/walmart... sorry to ask i just want to be quick im in town and i am about to hit traffic if i dobt head home soon.. if anything else is needed i need to get it asap. I already got malt dextros and peitri dishes


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OfflineThe Mycologist
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23839167 - 11/16/16 03:24 PM (7 years, 2 months ago)

Scalpel maybe


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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InvisibleComebackKid
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Re: Questionable mycelium [Re: The Mycologist]
    #23839216 - 11/16/16 03:38 PM (7 years, 2 months ago)

Inoc loop maybe


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: ComebackKid]
    #23839258 - 11/16/16 03:50 PM (7 years, 2 months ago)

You fkin dudes ROCK! Got my pietri dishes and malt extract agar... gonna start my research at home.. im a little confused though cas i glanced at that pasteywhite tek and it seems i need agar agar, whatever that is? Lol im hoping i dont need to turn around?
Can i be recomended a tek which will work with nothing else from the sci store, aside from my malt extract agar and pietris... this is a little overwelming... like the first time i grew BRF or WBS. I know it will be simple once i do it cas im familiar with that situation... i just need to put my big boy pants on and maybe someone to hold my hand the first time (lol)


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InvisibleComebackKid
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23839272 - 11/16/16 03:53 PM (7 years, 2 months ago)

Your MEA is a premix of malt extract and agar agar powder.
Pasty tek gives you a recipe to make potato dextrose agar from scratch. You could make MEA from scratch too if you wanted. But with what you have now all you need to do is add water.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: ComebackKid]
    #23839301 - 11/16/16 04:00 PM (7 years, 2 months ago)

Hell yeah buddy! Can you recommend a retard-proof tek for MEA? I think this would be the best option for me rather than any mixing aside from water. Gotta keep it simple or might overwork my fragile brain at this point lol


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23839447 - 11/16/16 04:45 PM (7 years, 2 months ago)

All my searching is piinting toward creating MEA simular to what you explained with pasteywhites tek... but since im one step ahead as i purchased MEA, what's next? Im assuming i mix the MEA powder with water, boil, put in bottle, PC, then inside a SAB pour the PCed MEA directly into pietris, cool, then inoculate with spores? I cant find anything to back this up so yea a tek would be beautiful for my current supplies. Thanks a bunch


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InvisibleComebackKid
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Re: Questionable mycelium [Re: Shroomy_Mclovin]
    #23839457 - 11/16/16 04:46 PM (7 years, 2 months ago)



--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineShroomy_Mclovin
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Re: Questionable mycelium [Re: ComebackKid]
    #23839483 - 11/16/16 04:57 PM (7 years, 2 months ago)

Ugh i suck! So that basically tells me what ratios to mix. But a step by step tek would make me comfortable. I just dont know, do i put one drop from my spore syringe, were my steps i previously mentioned correct? Sorry i keep asking questions im just afraid of ruining the last lil bit of spores i have on hand...


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