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Offlinebrassmonkey
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Registered: 02/06/16
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Safe to use clone tissue from tammed petri?
    #23834547 - 11/15/16 06:22 AM (7 years, 2 months ago)

Hey guys. This is my first time working with agar and I had a question. I have 3 Petri dishes going with clone tissue from a previous grow, they have been colonizing the petri for 2 weeks. 2 of the 3 dishes have contaminates and I am going to transfer a sample of the clone tissue onto a new dish. My question is, is it safe to go ahead and use some of the tissue to noc up some grain spawn or is that a bad move because of the contaminate present in the dish? Thanks for your help!



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Offlineenlightenment
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Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
Re: Safe to use clone tissue from tammed petri? [Re: brassmonkey]
    #23834563 - 11/15/16 06:41 AM (7 years, 2 months ago)

Try to transfer a healthy part of the contaminated plate to a new dish (within a SAB). Wait until it grows out a bit and use the new grown mycelium to inoculate a jar. (Contams could be still present invisible. An additional transfer for extra safety is recommended)


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