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Edmunter
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Pleurotus eryngii first time
#23834450 - 11/15/16 04:15 AM (7 years, 2 months ago) |
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I am growing this for the first time and wanted to know what methods you have had success with and why?
I took a sample from a supermarket bought fruit and believe that would be close to an Isolate? I have 3 cultures growing on plates and thought about making an LI, a LC and agar wedge to grain.
Just for the info, does an isolate fruit only carry that one genetic? What happens when it sporulates, does it create loads of multi spores?
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Ferather
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Re: Pleurotus eryngii first time [Re: Edmunter]
#23834786 - 11/15/16 09:04 AM (7 years, 2 months ago) |
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Its sort of an isolate if you pick from a bunch of fruits. The normal term in this case is clone. An isolate is generally more in-depth, such as isolating a growing section on agar.
LI would be fine. Skip LC, sugars are risky and difficult to work with. If a mushroom sporulates it produces random genetics.
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Edmunter
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Re: Pleurotus eryngii first time [Re: Ferather]
#23834831 - 11/15/16 09:19 AM (7 years, 2 months ago) |
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I understand about clones but how about isolates, if you clone them you are guaranteed to get that mushroom again but does it still have the ability to create multi spores?
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Ferather
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Re: Pleurotus eryngii first time [Re: Edmunter]
#23834848 - 11/15/16 09:26 AM (7 years, 2 months ago) |
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Yes it does. Your clone will be running dominate genetics, when it makes spores it will use both dominant and recessive genes. Lets say you have two children with the same woman, one male: blond, blue eyes, one female: brunette, brown eyes.
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Marty Mycfly
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Registered: 12/16/13
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Re: Pleurotus eryngii first time [Re: Ferather]
#23835290 - 11/15/16 12:03 PM (7 years, 2 months ago) |
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Hey Edmunter, I grow eryngii, hands down the best way to grow kings with good BE is bag culture on 20% supplemented sawdust. In short, your sub is hardwood sawdust or wood pellets and 20% wheat bran, you sterilize that and inoculate with grain spawn. They should incubate for 3 weeks and then given a week of cold temps between 50-60 degrees. They will pin invitro in the cold, after inducing a pin set you will cut the bags open leaving a wall around the block and it's ready to fruit. They like colder temps, I leave my grow room at 68 degrees and set RH at 85%. Check out the link in my sig about King Oysters.
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Edmunter
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Re: Pleurotus eryngii first time [Re: Marty Mycfly]
#23835305 - 11/15/16 12:06 PM (7 years, 2 months ago) |
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Quote:
Marty Mycfly said: Hey Edmunter, I grow eryngii, hands down the best way to grow kings with good BE is bag culture on 20% supplemented sawdust. In short, your sub is hardwood sawdust or wood pellets and 20% wheat bran, you sterilize that and inoculate with grain spawn. They should incubate for 3 weeks and then given a week of cold temps between 50-60 degrees. They will pin invitro in the cold, after inducing a pin set you will cut the bags open leaving a wall around the block and it's ready to fruit. They like colder temps, I leave my grow room at 68 degrees and set RH at 85%. Check out the link in my sig about King Oysters.
That is exactly what I was after. Thank you.
So it is possible to do it in a mono tub too as I dont have a GH. I will experiment
Edited by Edmunter (11/15/16 12:08 PM)
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Marty Mycfly
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Re: Pleurotus eryngii first time [Re: Edmunter]
#23835329 - 11/15/16 12:15 PM (7 years, 2 months ago) |
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I have seen King monos, though I wouldnt say it's optimal for them, these are FAE sucking monsters. Also they do extremely better on supplemented sub which is hard to get into a monotub I presume, but just growing any amount of kings is fun. I built my first greenhouse because of kings, it's a really rewarding mushroom to grow, I wouldnt be surprised if you build one after you get your first taste of your own kings.
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Ferather
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Re: Pleurotus eryngii first time [Re: Marty Mycfly]
#23835378 - 11/15/16 12:32 PM (7 years, 2 months ago) |
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The mono idea works best with no lid, so it works the same way as your design Marty. Just pre-measure the depth, place holes near the top of the monotub.
Use the lid for the pinning inducing, then remove it.
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Marty Mycfly
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Re: Pleurotus eryngii first time [Re: Ferather]
#23835391 - 11/15/16 12:39 PM (7 years, 2 months ago) |
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There you go
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Edmunter
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Re: Pleurotus eryngii first time [Re: Ferather]
#23835397 - 11/15/16 12:42 PM (7 years, 2 months ago) |
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So I would either have to colonise in a bag and then transfer to mono for a suplement or not use bran.
I have a spare sgfc hanging around I may dip a bag in also.
Cultures are growing fast.
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Ferather
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Re: Pleurotus eryngii first time [Re: Marty Mycfly]
#23835401 - 11/15/16 12:44 PM (7 years, 2 months ago) |
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I take no credit, I based it from your design and a pre-existing monotub.
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Edmunter
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Re: Pleurotus eryngii first time [Re: Ferather]
#23835417 - 11/15/16 12:52 PM (7 years, 2 months ago) |
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How did u colonise it , ratios, sub ect?
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Marty Mycfly
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Re: Pleurotus eryngii first time [Re: Ferather]
#23835423 - 11/15/16 12:54 PM (7 years, 2 months ago) |
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I would just do unsupplemented for the monotub, and the SGFC would work great with a bag. Look forward to seeing it
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Edmunter
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Re: Pleurotus eryngii first time [Re: Marty Mycfly]
#23835430 - 11/15/16 12:55 PM (7 years, 2 months ago) |
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So one pasteurised and the other sterilised.
I just had mushrooms on toast....... be nice with these guys
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Ferather
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Re: Pleurotus eryngii first time [Re: Edmunter]
#23835438 - 11/15/16 12:59 PM (7 years, 2 months ago) |
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Hang on, let me reiterate, which tek is being used, the SGFC version or the mono?
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Edmunter
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Re: Pleurotus eryngii first time [Re: Ferather]
#23835440 - 11/15/16 01:00 PM (7 years, 2 months ago) |
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Both, mini mono and a bag
Edited by Edmunter (11/15/16 01:00 PM)
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Ferather
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Re: Pleurotus eryngii first time [Re: Edmunter]
#23835450 - 11/15/16 01:03 PM (7 years, 2 months ago) |
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Ah ok, my bad. The mono I made based on Marty's design is almost a SGFC. I will draw one in a few minutes. The bag in a SGFC should work great, if you fill the surroundings with hydrated perlite.
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Marty Mycfly
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Re: Pleurotus eryngii first time [Re: Edmunter]
#23835452 - 11/15/16 01:04 PM (7 years, 2 months ago) |
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Quote:
Edmunter said: So one pasteurised and the other sterilised.
I just had mushrooms on toast....... be nice with these guys
Sounds like a good plan, and you get to see how your culture performs on different subs, how far are you out from spawn and how are you going to go about it?
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Edmunter
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Re: Pleurotus eryngii first time [Re: Marty Mycfly]
#23835476 - 11/15/16 01:13 PM (7 years, 2 months ago) |
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Just transferred from clone plate so if it clean put one wedge in a jar and maybe LI with the other.
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Ferather
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Re: Pleurotus eryngii first time [Re: Edmunter]
#23835494 - 11/15/16 01:22 PM (7 years, 2 months ago) |
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Colonize your substrate in the tub, holes filled, when colonized, case in clean compost, remove the filler. When you see lots of mushroom pinning all around the surface, remove the lid completely.
Maximum of 4 inches (10.16 cm), surface to top, right Marty?
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