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Sirtalis



Registered: 10/05/16
Posts: 409
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Dry mushroom to agar attempt
#23832177 - 11/14/16 01:02 PM (7 years, 2 months ago) |
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Hello! This is my first time using agar. I have some cubensis lying around so I decided to try to clone the dry tissue. Made the agar extra wet for this experiment. I'm not expecting much, but maybe after a few weeks mycelium will start to grow.
I also am attempting to clone some Psycolobe allenii on agar. We'll see how that goes.
Anyway, here are some pictures. I'll keep this thread updated on wether or not either of these worked.
Psycolobe allenii

Psycolobe cubensis
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Nobler Hino
a dojo and a forge?!


Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
#23832198 - 11/14/16 01:07 PM (7 years, 2 months ago) |
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Shoulda went with gill tissue but we'll see what happens, welcome to the shroomery dude.
--------------------
   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.” – Maria Sabina
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Sirtalis



Registered: 10/05/16
Posts: 409
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Re: Dry mushroom to agar attempt [Re: Nobler Hino]
#23832209 - 11/14/16 01:10 PM (7 years, 2 months ago) |
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Thanks, this place is awesome!
I have a couple other plates with gill tissue. Wanted to test out each part of the mushroom just to see which performs best. Used stem, gill and inside the caps on different agar plates.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
#23832340 - 11/14/16 01:56 PM (7 years, 2 months ago) |
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As long as they were dried gently it should be fine...good luck.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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SporeDaddy
Daddy to my Spores



Registered: 08/20/16
Posts: 92
Loc: Classified
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Re: Dry mushroom to agar attempt [Re: cronicr]
#23832361 - 11/14/16 02:04 PM (7 years, 2 months ago) |
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-------------------- "Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom." - Terrence McKenna  
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Dry mushroom to agar attempt [Re: SporeDaddy] 1
#23832650 - 11/14/16 03:25 PM (7 years, 2 months ago) |
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Good luck with the bacteria tho. I doubt it was fruited and dried sterile.
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
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Re: Dry mushroom to agar attempt [Re: Pastywhyte]
#23832819 - 11/14/16 04:21 PM (7 years, 2 months ago) |
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Following, Bc fuck it why not
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Sirtalis



Registered: 10/05/16
Posts: 409
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Re: Dry mushroom to agar attempt [Re: Th3Issu3]
#23834153 - 11/14/16 10:51 PM (7 years, 2 months ago) |
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Probably going to be a bacteria bonanza but there's a chance it will work. The cubensis have been sitting in a ziploc for 8 months. The allenii were freshly picked. Those suckers turned dark blue on agar after 24 hours. Some serious psilocin.
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
#23834160 - 11/14/16 10:56 PM (7 years, 2 months ago) |
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Quote:
Sirtalis said: Probably going to be a bacteria bonanza but there's a chance it will work. The cubensis have been sitting in a ziploc for 8 months. The allenii were freshly picked. Those suckers turned dark blue on agar after 24 hours. Some serious psilocin.
Does the blueness indicate alkaloid content?
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
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Sirtalis



Registered: 10/05/16
Posts: 409
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Stamets writes: "Apparently, the bluing pigmentation is a result of a phenomenon paralleling the degradation of unstable psilocin (dephosphorylated psilocybin) to presently unknown compounts by enzymes within the mushroom cells."
I blended up a few mature allenii to make a slurry and it was royal blue. Pretty crazy. I'm not 100% convinced bluing has to do with potency, but it's possible it is an indicator.
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
#23834192 - 11/14/16 11:16 PM (7 years, 2 months ago) |
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Interesting. I mean I obviously have observed the "blue" but never correlated it to alkaloid content. Far out!
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
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Sirtalis



Registered: 10/05/16
Posts: 409
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Bacteria bonanza is in full effect. The cubensis are surrounded by bacteria but the cloned allenii are off to an awesome start.
Notes for next time: 1. Don't make the agar super fucking wet. Hydrate the tissue beforehand instead of dealing with a soupy mess.
2. Dip the dried tissue in h2o2. The benefit of preventing bacteria far outweighs the damage done to the mycelium from h2o2.
3. Try using the gill instead of the stem.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
#23836487 - 11/15/16 06:39 PM (7 years, 2 months ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Sirtalis



Registered: 10/05/16
Posts: 409
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Re: Dry mushroom to agar attempt [Re: cronicr]
#23836533 - 11/15/16 06:52 PM (7 years, 2 months ago) |
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Very nice, the 3 water baths should help my chances. Thank you for sharing your link.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
#23836550 - 11/15/16 06:59 PM (7 years, 2 months ago) |
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Good Luck! May the contaminates not be with you
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
#23836557 - 11/15/16 07:01 PM (7 years, 2 months ago) |
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An alternative to dipping in peroxide might be a 1:10 bleach solution. It doesn't stunt the mycelium as badly, but will give you a leg up on bacteria and mold spores.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Dry mushroom to agar attempt [Re: r.lutece]
#23836562 - 11/15/16 07:02 PM (7 years, 2 months ago) |
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ime bacteria can be a nasty bitch...
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Dry mushroom to agar attempt [Re: Kenetic]
#23836648 - 11/15/16 07:32 PM (7 years, 2 months ago) |
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Following this curious
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Quote:
A.RichardTrickle said:
Quote:
Sirtalis said: Probably going to be a bacteria bonanza but there's a chance it will work. The cubensis have been sitting in a ziploc for 8 months. The allenii were freshly picked. Those suckers turned dark blue on agar after 24 hours. Some serious psilocin.
Does the blueness indicate alkaloid content?
No. Blueing is just an indicator that you might have a psychedelic mushroom in your hands. There are edible bolete mushrooms that bruise blue and have zero psychedelic compounds. There are also people on here that have grown PE that didn't blue much at all yet would melt your face off. I believe it was pasty that said the bunkest shrooms he ever grew was PE. I bet his bruised blue.
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
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Re: Dry mushroom to agar attempt [Re: Juiceh]
#23837087 - 11/15/16 10:08 PM (7 years, 2 months ago) |
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Huh, interesting, thanks. I'd love to find another decent PE swab.
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
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Sirtalis



Registered: 10/05/16
Posts: 409
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So the dry cubensis and dry allenii are completely covered in bacteria. I threw those out.
The fresh allenii are ok for the moment. Some pieces have bacteria surrounding them. Can I get some advice on how/when to transfer these to new agar plates? For most of the pieces the mycelium seems to be airborne, and the mycelium touching the agar plate is not taking off as well. From the looks of it, the chunks in contact with the agar are slightly contaminated with bacteria. Should I try to scrape the "airborne" mycelium onto new plates to clean it up? Any input helps.

Hard to tell from this photo but you can kind of make out that the chunk in contact with agar has bacteria.

Here is the plate upside down:
Edited by Sirtalis (11/17/16 04:49 PM)
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
#23842819 - 11/17/16 05:55 PM (7 years, 2 months ago) |
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Agar sandwich.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Dry mushroom to agar attempt [Re: van hatton]
#23842863 - 11/17/16 06:04 PM (7 years, 2 months ago) |
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Gunna say what I said on his contam forum post..
Quote:
Mad Season said: Is it just me or does any one else see like barely any bacteria? Like only a few spots on the 3rd plate.. I'd say just give it some more time. It's looking like it's just recovering to me.
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mojoejojo
Anonankan

Registered: 05/26/17
Posts: 13
Loc: Earth
Last seen: 23 days, 9 hours
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Re: Dry mushroom to agar attempt [Re: van hatton]
#24454246 - 07/03/17 10:54 AM (6 years, 6 months ago) |
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How did it go??
Quote:
van hatton said: Agar sandwich.
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molemole
Ethnobotic



Registered: 04/14/12
Posts: 465
Loc: PNW
Last seen: 9 days, 21 hours
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Re: Dry mushroom to agar attempt [Re: Nobler Hino]
#24454420 - 07/03/17 12:19 PM (6 years, 6 months ago) |
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Quote:
Nobler Hino said: Shoulda went with gill tissue but we'll see what happens, welcome to the shroomery dude.
I just saw this thread. I have successfully grown from dried azurescens fruits. I picked the fruits in the wild from the washington coast. Dehydrated without taking prints. I was a noob and didn't think they would grow outside of the natural environment.
I realized I could grow them in the puget sound area. So I decided to try and get soores from the gills of the dehydrated fruits that were about 6 months old. I put the fruit, a pair of tweezers and a petri dish in the sab. I flame sterilized the tweezers and pluck a small piece of the gill tissue. Then placed it on the dish. It grew first try and after a few transfers was clean. This process resulted in the azzies in my avatar. They are still going and I just add new chips in the winter. I tried getting a culture from colonized woodchips on agar that was a fail every time.
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mojoejojo
Anonankan


Registered: 05/26/17
Posts: 13
Loc: Earth
Last seen: 23 days, 9 hours
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Re: Dry mushroom to agar attempt [Re: molemole]
#25531335 - 10/12/18 03:28 AM (5 years, 3 months ago) |
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Snap!
-------------------- My skills are under construction
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