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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Dry mushroom to agar attempt [Re: A.RichardTrickle]
    #23842536 - 11/17/16 04:48 PM (7 years, 2 months ago)

So the dry cubensis and dry allenii are completely covered in bacteria.  I threw those out.

The fresh allenii are ok for the moment.  Some pieces have bacteria surrounding them.  Can I get some advice on how/when to transfer these to new agar plates?  For most of the pieces the mycelium seems to be airborne, and the mycelium touching the agar plate is not taking off as well.  From the looks of it, the chunks in contact with the agar are slightly contaminated with bacteria.  Should I try to scrape the "airborne" mycelium onto new plates to clean it up?  Any input helps.





Hard to tell from this photo but you can kind of make out that the chunk in contact with agar has bacteria.



Here is the plate upside down:


Edited by Sirtalis (11/17/16 04:49 PM)


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Invisiblevan hattonFacebookDiscord
Still a noob
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Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
    #23842819 - 11/17/16 05:55 PM (7 years, 2 months ago)

Agar sandwich.


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Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: Dry mushroom to agar attempt [Re: van hatton]
    #23842863 - 11/17/16 06:04 PM (7 years, 2 months ago)

Gunna say what I said on his contam forum post..

Quote:

Mad Season said:
Is it just me or does any one else see like barely any bacteria? Like only a few spots on the 3rd plate.. I'd say just give it some more time. It's looking like it's just recovering to me.




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Offlinemojoejojo
Anonankan
I'm a teapot
Registered: 05/26/17
Posts: 13
Loc: Earth
Last seen: 23 days, 7 hours
Re: Dry mushroom to agar attempt [Re: van hatton]
    #24454246 - 07/03/17 10:54 AM (6 years, 6 months ago)

How did it go??





Quote:

van hatton said:
Agar sandwich.




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Offlinemolemole
Ethnobotic
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Registered: 04/14/12
Posts: 465
Loc: PNW
Last seen: 9 days, 19 hours
Re: Dry mushroom to agar attempt [Re: Nobler Hino]
    #24454420 - 07/03/17 12:19 PM (6 years, 6 months ago)

Quote:

Nobler Hino said:
Shoulda went with gill tissue but we'll see what happens, welcome to the shroomery dude.



I just saw this thread. I have successfully grown from dried azurescens fruits. I picked the fruits in the wild from the washington coast. Dehydrated without taking prints. I was a noob and didn't think they would grow outside of the natural environment. 

I realized I could grow them in the puget sound area.  So I decided to try and get soores from the gills of the dehydrated fruits that were about 6 months old. I put the fruit,  a pair of tweezers and a petri dish in the sab. I flame sterilized the tweezers and pluck a small piece of the gill tissue. Then placed it on the dish. It grew first try and after a few transfers was clean. This process resulted in the azzies in my avatar. They are still going and I just add new chips in the winter. I tried getting a culture from colonized woodchips on agar that was a fail every time.


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Offlinemojoejojo
Anonankan
I'm a teapot

Registered: 05/26/17
Posts: 13
Loc: Earth
Last seen: 23 days, 7 hours
Re: Dry mushroom to agar attempt [Re: molemole]
    #25531335 - 10/12/18 03:28 AM (5 years, 3 months ago)

Snap!


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My skills are under construction :stoned:


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