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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Dry mushroom to agar attempt
    #23832177 - 11/14/16 01:02 PM (7 years, 2 months ago)

Hello!  This is my first time using agar.  I have some cubensis lying around so I decided to try to clone the dry tissue.  Made the agar extra wet for this experiment.  I'm not expecting much, but maybe after a few weeks mycelium will start to grow. 

I also am attempting to clone some Psycolobe allenii on agar.  We'll see how that goes.

Anyway, here are some pictures.  I'll keep this thread updated on wether or not either of these worked.

Psycolobe allenii


Psycolobe cubensis


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InvisibleNobler Hino
a dojo and a forge?!
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Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Dry mushroom to agar attempt [Re: Sirtalis]
    #23832198 - 11/14/16 01:07 PM (7 years, 2 months ago)

Shoulda went with gill tissue but we'll see what happens, welcome to the shroomery dude.


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Dry mushroom to agar attempt [Re: Nobler Hino]
    #23832209 - 11/14/16 01:10 PM (7 years, 2 months ago)

Thanks, this place is awesome! 

I have a couple other plates with gill tissue.  Wanted to test out each part of the mushroom just to see which performs best.  Used stem, gill and inside the caps on different agar plates.


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 9 days
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
    #23832340 - 11/14/16 01:56 PM (7 years, 2 months ago)

As long as they were dried gently it should be fine...good luck.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleSporeDaddy
Daddy to my Spores
I'm a teapot User Gallery


Registered: 08/20/16
Posts: 92
Loc: Classified
Re: Dry mushroom to agar attempt [Re: cronicr]
    #23832361 - 11/14/16 02:04 PM (7 years, 2 months ago)

:whathesaid:


--------------------
"Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom."
- Terrence McKenna

:nicetry::heytherebadboy:


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,810
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Re: Dry mushroom to agar attempt [Re: SporeDaddy] * 1
    #23832650 - 11/14/16 03:25 PM (7 years, 2 months ago)

Good luck with the bacteria tho. I doubt it was fruited and dried sterile.


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OfflineTh3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
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Re: Dry mushroom to agar attempt [Re: Pastywhyte]
    #23832819 - 11/14/16 04:21 PM (7 years, 2 months ago)

Following, Bc fuck it why not


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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Dry mushroom to agar attempt [Re: Th3Issu3]
    #23834153 - 11/14/16 10:51 PM (7 years, 2 months ago)

Probably going to be a bacteria bonanza but there's a chance it will work.  The cubensis have been sitting in a ziploc for 8 months.  The allenii were freshly picked.  Those suckers turned dark blue on agar after 24 hours.  Some serious psilocin.


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OfflineA.RichardTrickle
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Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
Re: Dry mushroom to agar attempt [Re: Sirtalis]
    #23834160 - 11/14/16 10:56 PM (7 years, 2 months ago)

Quote:

Sirtalis said:
Probably going to be a bacteria bonanza but there's a chance it will work.  The cubensis have been sitting in a ziploc for 8 months.  The allenii were freshly picked.  Those suckers turned dark blue on agar after 24 hours.  Some serious psilocin.



Does the blueness indicate alkaloid content?


--------------------
"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
"If you're making love to your old lady, someone else is fucking her"
"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Dry mushroom to agar attempt [Re: A.RichardTrickle]
    #23834170 - 11/14/16 11:02 PM (7 years, 2 months ago)

Stamets writes: "Apparently, the bluing pigmentation is a result of a phenomenon paralleling the degradation of unstable psilocin (dephosphorylated psilocybin) to presently unknown compounts by enzymes within the mushroom cells."

I blended up a few mature allenii to make a slurry and it was royal blue.  Pretty crazy.  I'm not 100% convinced bluing has to do with potency, but it's possible it is an indicator.


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OfflineA.RichardTrickle
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Registered: 11/04/16
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
    #23834192 - 11/14/16 11:16 PM (7 years, 2 months ago)

Interesting.
I mean I obviously have observed the "blue" but never correlated it to alkaloid content.
Far out!


--------------------
"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
"If you're making love to your old lady, someone else is fucking her"
"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Dry mushroom to agar attempt [Re: A.RichardTrickle]
    #23836473 - 11/15/16 06:34 PM (7 years, 2 months ago)

Bacteria bonanza is in full effect.  The cubensis are surrounded by bacteria but the cloned allenii are off to an awesome start.

Notes for next time:
1. Don't make the agar super fucking wet.  Hydrate the tissue beforehand instead of dealing with a soupy mess.

2. Dip the dried tissue in h2o2.  The benefit of preventing bacteria far outweighs the damage done to the mycelium from h2o2.

3.  Try using the gill instead of the stem.


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OfflinecronicrFacebook
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
    #23836487 - 11/15/16 06:39 PM (7 years, 2 months ago)



--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleSirtalis
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Registered: 10/05/16
Posts: 409
Re: Dry mushroom to agar attempt [Re: cronicr]
    #23836533 - 11/15/16 06:52 PM (7 years, 2 months ago)

Very nice, the 3 water baths should help my chances.  Thank you for sharing your link.


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OfflineKenetic
Nam Sayin
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Registered: 08/24/14
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Re: Dry mushroom to agar attempt [Re: Sirtalis]
    #23836550 - 11/15/16 06:59 PM (7 years, 2 months ago)

:popcorn:

Good Luck!  May the contaminates not be with you


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Invisibler.lutece
gave Columbia her wings.
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Registered: 09/06/15
Posts: 745
Loc: ∅
Re: Dry mushroom to agar attempt [Re: Sirtalis]
    #23836557 - 11/15/16 07:01 PM (7 years, 2 months ago)

An alternative to dipping in peroxide might be a 1:10 bleach solution. It doesn't stunt the mycelium as badly, but will give you a leg up on bacteria and mold spores.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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OfflineKenetic
Nam Sayin
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Registered: 08/24/14
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Re: Dry mushroom to agar attempt [Re: r.lutece]
    #23836562 - 11/15/16 07:02 PM (7 years, 2 months ago)

ime bacteria can be a nasty bitch...


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Invisiblevan hattonFacebookDiscord
Still a noob
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Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Dry mushroom to agar attempt [Re: Kenetic]
    #23836648 - 11/15/16 07:32 PM (7 years, 2 months ago)

Following this curious


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisibleJuiceh
Dabbing All Day
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Registered: 09/25/12
Posts: 3,208
Re: Dry mushroom to agar attempt [Re: A.RichardTrickle]
    #23837043 - 11/15/16 09:56 PM (7 years, 2 months ago)

Quote:

A.RichardTrickle said:
Quote:

Sirtalis said:
Probably going to be a bacteria bonanza but there's a chance it will work.  The cubensis have been sitting in a ziploc for 8 months.  The allenii were freshly picked.  Those suckers turned dark blue on agar after 24 hours.  Some serious psilocin.



Does the blueness indicate alkaloid content?



No. Blueing is just an indicator that you might have a psychedelic mushroom in your hands. There are edible bolete mushrooms that bruise blue and have zero psychedelic compounds. There are also people on here that have grown PE that didn't blue much at all yet would melt your face off. I believe it was pasty that said the bunkest shrooms he ever grew was PE. I bet his bruised blue.


--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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OfflineA.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
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Re: Dry mushroom to agar attempt [Re: Juiceh]
    #23837087 - 11/15/16 10:08 PM (7 years, 2 months ago)

Huh, interesting, thanks.
I'd love to find another decent PE swab.


--------------------
"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
"If you're making love to your old lady, someone else is fucking her"
"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


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