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Offlinecolumbo
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Registered: 07/20/16
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Inoculate to bird seed.When to shake?
    #23831059 - 11/14/16 02:21 AM (7 years, 2 months ago)

Hi everyone,I inoculated mycelium to bird seed first time,I shoke it after that.Do I need to shake it again?it's 11 days passed...
Thank You


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Offlinetump
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Registered: 03/17/16
Posts: 2,383
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
    #23831094 - 11/14/16 03:09 AM (7 years, 2 months ago)

Shake only at 30% colonised and when fully colonised two days before spawning to see if recoving well.


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Invisible2shoes
The anti-agar
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Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
Re: Inoculate to bird seed.When to shake? [Re: tump]
    #23831157 - 11/14/16 04:21 AM (7 years, 2 months ago)

It's not recommended to shake more than twice. Once at inoculation then again once around 30% colonized. No need to shake to see recovery.

That jar looks kind of sketch. Maybe it's the haze on the glass but how was your mycelium produced? Agar wedge, Agar to lc, spore to lc?


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Offlinewtfcrazymofo
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Re: Inoculate to bird seed.When to shake? [Re: 2shoes]
    #23831174 - 11/14/16 04:33 AM (7 years, 2 months ago)

Shake once after you drop the wedge.


--------------------
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Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Invisible2shoes
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Re: Inoculate to bird seed.When to shake? [Re: wtfcrazymofo]
    #23831202 - 11/14/16 05:05 AM (7 years, 2 months ago)

Then again once it's around 30%


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Offlinecolumbo
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Re: Inoculate to bird seed.When to shake? [Re: wtfcrazymofo]
    #23832321 - 11/14/16 01:51 PM (7 years, 2 months ago)

It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...


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InvisibleSporeDaddy
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Registered: 08/20/16
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
    #23832354 - 11/14/16 02:01 PM (7 years, 2 months ago)

Quote:

columbo said:
It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...




They are right to say only shake after inoculation to spread agar and again once you are ready to spawn into substrate. Your jar looks good in my opinion i can see healthy white mycelium colonizing at least 30% maybe more.

I say wait several more days (3 days) to let it really spread cause it will grow fast once it has large enough area covered. Then follow a tek into spawning it and fruiting.

Goodluck! Keep updating with pics:mushroom2:


--------------------
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- Terrence McKenna

:nicetry::heytherebadboy:


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Offlinecolumbo
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Re: Inoculate to bird seed.When to shake? [Re: SporeDaddy]
    #23832359 - 11/14/16 02:03 PM (7 years, 2 months ago)

Thank you :smile:


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Invisiblenooneman
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
    #23832368 - 11/14/16 02:06 PM (7 years, 2 months ago)

That jar looks contaminated. In the future, don't use birdseed. Birdseed is just about the worst and most contaminate prone substrate there is. Use grain instead.


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InvisibleSporeDaddy
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Registered: 08/20/16
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Re: Inoculate to bird seed.When to shake? [Re: nooneman]
    #23832391 - 11/14/16 02:13 PM (7 years, 2 months ago)

OP's birdseed looks like a grain based mix possibly, and his jar is very greasy which is what makes it look contaminated. Too hard to tell with that one angle. I examined the picture at full screen.


--------------------
"Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom."
- Terrence McKenna

:nicetry::heytherebadboy:


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Offlinecolumbo
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
    #23832468 - 11/14/16 02:34 PM (7 years, 2 months ago)

I think it is just some glue left after labels came off in PC,I use old school jars from ketchup made in postsovietik country :laugh: just didn't have time to order proper ones :smile:But you an be right,we'll see :smile: thanks


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OfflinePinPornProducer
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
    #23832490 - 11/14/16 02:37 PM (7 years, 2 months ago)

Quote:

columbo said:
It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...



Spores to lc :justno:


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OfflineTira
I'm a teapot


Registered: 11/20/10
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
    #23832512 - 11/14/16 02:42 PM (7 years, 2 months ago)

Quote:

columbo said:
It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...




With LC, shaking once right after inoculation is enough.  You can do the same with agar but I like to wait until %25 colonization then shake once.

Btw they don't look like WBS, those grains look huge, what kind of birdseed are they?

and this:

Quote:

PinPornProducer said:

Spores to lc :justno:




Next time try achieveing clean culture with spores -> agar and make transfers if necessary, and then agar -> LC.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


Edited by Tira (11/14/16 02:44 PM)


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Offlinecolumbo
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Re: Inoculate to bird seed.When to shake? [Re: Tira]
    #23832538 - 11/14/16 02:50 PM (7 years, 2 months ago)

I just dont know all right terms,but fuk it I'm learning:) I ment that LC was spores to water/honey :smile:
Quote:

Tira said:
Quote:

columbo said:
It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...




With LC, shaking once right after inoculation is enough.  You can do the same with agar but I like to wait until % colonization then shake once.

Btw they don't look like WBS, those grains look huge, what kind of birdseed are they?

and this:

Quote:

PinPornProducer said:

Spores to lc :justno:




Next time try achieveing clean culture with spores -> agar and make transfers if necessary, and then agar -> LC.




Wow thanks for the tip,I thought LC does the same as agar or spores ...
What is the difference shortly?size of the fruit , potency ?


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Offlinecolumbo
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
    #23832544 - 11/14/16 02:52 PM (7 years, 2 months ago)

I mean whats wrong with spores to LC?


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OfflinePinPornProducer
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
    #23832548 - 11/14/16 02:54 PM (7 years, 2 months ago)

It's a good way to grow alot junk very fast


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OfflineTira
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Registered: 11/20/10
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
    #23834414 - 11/15/16 03:34 AM (7 years, 2 months ago)

Quote:

columbo said:
I mean whats wrong with spores to LC?




Spores prints and syringes are not sterile, so as long as you don't start and clean the culture on agar, you might be inoculating bacteria or mold spores along with your intended spores.  What you're doing when creating spawn is expanding a culture, and if that culture is not clean, that means you're also expanding contamination; and because bacteria or molds expand way faster than our intended myceliums, it would not be a good step.

(that means spores to grains is also a bad idea, but it's a little bit better than LC as with LC you're expanding the culture before you transfer it to grain)

Also, LC is basically sugars mixed with water so it's a perfect medium for bacteria.  You gotta be sure it's only your intended culture you're putting in there. .)


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


Edited by Tira (11/15/16 03:37 AM)


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