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columbo
Stranger

Registered: 07/20/16
Posts: 66
Last seen: 3 years, 7 months
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Inoculate to bird seed.When to shake?
#23831059 - 11/14/16 02:21 AM (7 years, 2 months ago) |
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Hi everyone,I inoculated mycelium to bird seed first time,I shoke it after that.Do I need to shake it again?it's 11 days passed... Thank You
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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
#23831094 - 11/14/16 03:09 AM (7 years, 2 months ago) |
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Shake only at 30% colonised and when fully colonised two days before spawning to see if recoving well.
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2shoes
The anti-agar



Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
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Re: Inoculate to bird seed.When to shake? [Re: tump]
#23831157 - 11/14/16 04:21 AM (7 years, 2 months ago) |
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It's not recommended to shake more than twice. Once at inoculation then again once around 30% colonized. No need to shake to see recovery.
That jar looks kind of sketch. Maybe it's the haze on the glass but how was your mycelium produced? Agar wedge, Agar to lc, spore to lc?
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wtfcrazymofo
foil hater



Registered: 07/26/15
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Re: Inoculate to bird seed.When to shake? [Re: 2shoes]
#23831174 - 11/14/16 04:33 AM (7 years, 2 months ago) |
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Shake once after you drop the wedge.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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2shoes
The anti-agar



Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
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Re: Inoculate to bird seed.When to shake? [Re: wtfcrazymofo]
#23831202 - 11/14/16 05:05 AM (7 years, 2 months ago) |
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Then again once it's around 30%
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columbo
Stranger

Registered: 07/20/16
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Re: Inoculate to bird seed.When to shake? [Re: wtfcrazymofo]
#23832321 - 11/14/16 01:51 PM (7 years, 2 months ago) |
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It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...
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SporeDaddy
Daddy to my Spores



Registered: 08/20/16
Posts: 92
Loc: Classified
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
#23832354 - 11/14/16 02:01 PM (7 years, 2 months ago) |
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Quote:
columbo said: It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...
They are right to say only shake after inoculation to spread agar and again once you are ready to spawn into substrate. Your jar looks good in my opinion i can see healthy white mycelium colonizing at least 30% maybe more.
I say wait several more days (3 days) to let it really spread cause it will grow fast once it has large enough area covered. Then follow a tek into spawning it and fruiting.
Goodluck! Keep updating with pics
-------------------- "Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom." - Terrence McKenna  
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columbo
Stranger

Registered: 07/20/16
Posts: 66
Last seen: 3 years, 7 months
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Re: Inoculate to bird seed.When to shake? [Re: SporeDaddy]
#23832359 - 11/14/16 02:03 PM (7 years, 2 months ago) |
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Thank you
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nooneman


Registered: 04/24/09
Posts: 14,568
Loc: Utah
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
#23832368 - 11/14/16 02:06 PM (7 years, 2 months ago) |
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That jar looks contaminated. In the future, don't use birdseed. Birdseed is just about the worst and most contaminate prone substrate there is. Use grain instead.
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SporeDaddy
Daddy to my Spores



Registered: 08/20/16
Posts: 92
Loc: Classified
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Re: Inoculate to bird seed.When to shake? [Re: nooneman]
#23832391 - 11/14/16 02:13 PM (7 years, 2 months ago) |
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OP's birdseed looks like a grain based mix possibly, and his jar is very greasy which is what makes it look contaminated. Too hard to tell with that one angle. I examined the picture at full screen.
-------------------- "Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom." - Terrence McKenna  
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columbo
Stranger

Registered: 07/20/16
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
#23832468 - 11/14/16 02:34 PM (7 years, 2 months ago) |
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I think it is just some glue left after labels came off in PC,I use old school jars from ketchup made in postsovietik country just didn't have time to order proper ones But you an be right,we'll see thanks
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
#23832490 - 11/14/16 02:37 PM (7 years, 2 months ago) |
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Quote:
columbo said: It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...
Spores to lc
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
#23832512 - 11/14/16 02:42 PM (7 years, 2 months ago) |
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Quote:
columbo said: It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...
With LC, shaking once right after inoculation is enough. You can do the same with agar but I like to wait until %25 colonization then shake once.
Btw they don't look like WBS, those grains look huge, what kind of birdseed are they?
and this:
Quote:
PinPornProducer said:
Spores to lc 
Next time try achieveing clean culture with spores -> agar and make transfers if necessary, and then agar -> LC.
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
Edited by Tira (11/14/16 02:44 PM)
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columbo
Stranger

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Re: Inoculate to bird seed.When to shake? [Re: Tira]
#23832538 - 11/14/16 02:50 PM (7 years, 2 months ago) |
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I just dont know all right terms,but fuk it I'm learning:) I ment that LC was spores to water/honey  Quote:
Tira said:
Quote:
columbo said: It is just bit dirty jar,I think it happened then I took off the label,nothing serious,It was spores to lc and lc to bird seed after.So does it look 30% already or you need better photo ? It is my first bird seed tek...
With LC, shaking once right after inoculation is enough. You can do the same with agar but I like to wait until % colonization then shake once.
Btw they don't look like WBS, those grains look huge, what kind of birdseed are they?
and this:
Quote:
PinPornProducer said:
Spores to lc 
Next time try achieveing clean culture with spores -> agar and make transfers if necessary, and then agar -> LC.
Wow thanks for the tip,I thought LC does the same as agar or spores ... What is the difference shortly?size of the fruit , potency ?
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columbo
Stranger

Registered: 07/20/16
Posts: 66
Last seen: 3 years, 7 months
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
#23832544 - 11/14/16 02:52 PM (7 years, 2 months ago) |
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I mean whats wrong with spores to LC?
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
#23832548 - 11/14/16 02:54 PM (7 years, 2 months ago) |
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It's a good way to grow alot junk very fast
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Inoculate to bird seed.When to shake? [Re: columbo]
#23834414 - 11/15/16 03:34 AM (7 years, 2 months ago) |
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Quote:
columbo said: I mean whats wrong with spores to LC?
Spores prints and syringes are not sterile, so as long as you don't start and clean the culture on agar, you might be inoculating bacteria or mold spores along with your intended spores. What you're doing when creating spawn is expanding a culture, and if that culture is not clean, that means you're also expanding contamination; and because bacteria or molds expand way faster than our intended myceliums, it would not be a good step.
(that means spores to grains is also a bad idea, but it's a little bit better than LC as with LC you're expanding the culture before you transfer it to grain)
Also, LC is basically sugars mixed with water so it's a perfect medium for bacteria. You gotta be sure it's only your intended culture you're putting in there. .)
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
Edited by Tira (11/15/16 03:37 AM)
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