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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
just to clarify........
    #23830972 - 11/14/16 12:50 AM (7 years, 2 months ago)

so MS to agar
drop 1-2 drops onto agar let colonize and transfer a clean section to a new plate.

so how long do I continue transfer exactly?

would i be better off cloning a fruit?(time wise)


Edited by Hallucyjunkie99 (11/14/16 12:54 AM)


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Offlineenlightenment
alchemist
 User Gallery

Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
Re: just to clarify........ [Re: Hallucyjunkie99]
    #23830993 - 11/14/16 01:11 AM (7 years, 2 months ago)

You just need one transfer to get the mycelium away from the spore solution. You can inoculate the grain with the colonized mycelium of the second dish if you want. Further transfers are made to isolate fast and healthy sectors.

I would recommend to use the second plate to inoculate grain.
- Grow it out until you have nice fruits.
- Clone the desired mushroom.
- Work with the clone

This is the best way if you are new to agar IMO.
Later you can try to isolate and test the isolates.

Cleaning and Isolating On Agar


Edited by enlightenment (11/14/16 01:18 AM)


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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: just to clarify........ [Re: enlightenment]
    #23831088 - 11/14/16 03:03 AM (7 years, 2 months ago)

Currently growing a mono  I'm planning on cloning a fruit on agar and making an isolate


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Invisible2shoes
The anti-agar
Male


Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
Re: just to clarify........ [Re: Hallucyjunkie99]
    #23831166 - 11/14/16 04:26 AM (7 years, 2 months ago)

Transfer as many times as you see fit either to clean it up or if it out grows the plate. A tissue clone will be faster to grow than germinating spores for sure. And you'll also have more of an idea how that genetics preform.


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