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Offlinepillbox
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Registered: 11/13/16
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Re: just starting out [Re: Plazmotech]
    #23833609 - 11/14/16 07:59 PM (7 years, 2 months ago)

Well I was thinking antibiotics to slow the growth of bacteria to allow the growth of spores than transfer away from bacteria.


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InvisibleMycolorado
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Re: just starting out [Re: Plazmotech]
    #23833624 - 11/14/16 08:02 PM (7 years, 2 months ago)

Torch loop til red hot.  Cool by dipping in agar of plate.  Rub loop over print.  Go back to plate and swipe in a zig zag pattern on agar.  You might not even see the spores that were just transferred...should have germination in around 7 days.  The amount of bacteria growing on your plates where the spores were deposited and the description of your techniques lead me to believe you are contaminating the plates and not the spores.  Wiping your loop and then transferring is most likely the cause if that is indeed what you were doing.  If not, it's probably some other area of your transfer technique.


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Offlinenumnum59
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Re: just starting out [Re: pillbox]
    #23833629 - 11/14/16 08:04 PM (7 years, 2 months ago)

I find syringes easier to noc up agar plates than spores themselves.
As for vectors, i feel your trading the noc loop for water


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Offlinepillbox
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Re: just starting out [Re: numnum59]
    #23833647 - 11/14/16 08:08 PM (7 years, 2 months ago)

I did flame sterlize the loop between dishes. I also cooled the loop by inserting into the agar.

I might need to get a thinner loop. Mine is a little thick.


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OfflineLeftfield420
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Re: just starting out [Re: Mycolorado]
    #23833666 - 11/14/16 08:13 PM (7 years, 2 months ago)

I almost suggested the exact same thing about 10 minutes ago!


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InvisibleMycolorado
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Re: just starting out [Re: numnum59]
    #23833688 - 11/14/16 08:18 PM (7 years, 2 months ago)

If you're just looking to dilute the spores I could see putting them in water in order to streak, but a syringe isn't necessary...and it's definitely easy to muck things up especially when you might be starting out and don't have good technique.  You might try both ways but either way, you should practice your technique and once you torch an instrument it's sterile until it touches something, or something touches it.  Just my 2.
I hope you get some cleaner results; good luck!


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Edited by Mycolorado (11/14/16 08:19 PM)


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OfflinecronicrFacebook
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Re: just starting out [Re: pillbox]
    #23833695 - 11/14/16 08:20 PM (7 years, 2 months ago)

Quote:

pillbox said:
Well I was thinking antibiotics to slow the growth of bacteria to allow the growth of spores than transfer away from bacteria.



Just make sure to eventually transfer to plain agar.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinepillbox
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Re: just starting out [Re: Mycolorado]
    #23833717 - 11/14/16 08:25 PM (7 years, 2 months ago)

http://www.ebay.com/itm/API-T-C-Tetracycline-Powder-Treat-bacterial-fish-disease-10pk-Freshwater-/252568885087?hash=item3ace47575f:g:TPIAAOSwoi1X8xtY

stable between 110-140C
but really think it was me inront of the flow hood?
I flame sterilized each time before I went to the spores. I made sure it was cool when I touched the agar. I got that nice sizzle sound.

I plan on only doing the spores on the anti bacteria agar than trannsfer myc to untreated agar.


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Offlinepillbox
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Re: just starting out [Re: pillbox]
    #23834278 - 11/15/16 12:27 AM (7 years, 2 months ago)



would this or a sterilize qtip work better for applying the spores


Edited by pillbox (11/15/16 01:00 AM)


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OfflineLeftfield420
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Re: just starting out [Re: pillbox]
    #23834582 - 11/15/16 07:05 AM (7 years, 2 months ago)

Either or...both should work....I use old tattoo needles for my loops..your loop should be fine..


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OfflinePlazmotech
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Re: just starting out [Re: pillbox]
    #23834633 - 11/15/16 07:48 AM (7 years, 2 months ago)

Quote:

pillbox said:


would this or a sterilize qtip work better for applying the spores




I'm fairly certain it should be just fine. But I've heard that inoculation loops should hold the surface tension of water. In other words, they should be small enough so when you dip them in water, a thin film of water should go across the loop.


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


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Offlinenumnum59
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Registered: 12/07/11
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Re: just starting out [Re: Plazmotech]
    #23835044 - 11/15/16 10:35 AM (7 years, 2 months ago)

I made mine from one of those pipe cleaner things for kids art. Just burned all the frilly shit off it and whabam


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OfflinePlazmotech
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Re: just starting out [Re: numnum59]
    #23835086 - 11/15/16 10:55 AM (7 years, 2 months ago)

HAHAHAHA creative


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


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OfflineA.RichardTrickle
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Re: just starting out [Re: numnum59]
    #23835421 - 11/15/16 12:54 PM (7 years, 2 months ago)

Quote:

numnum59 said:
I made mine from one of those pipe cleaner things for kids art. Just burned all the frilly shit off it and whabam



I'm going to try this.


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"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
"If you're making love to your old lady, someone else is fucking her"
"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


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InvisibleMycolorado
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Re: just starting out [Re: A.RichardTrickle]
    #23836186 - 11/15/16 04:47 PM (7 years, 2 months ago)

A paper clip will work...all you need is something you can flame sterilize (read: metal wire).  I used some kanthal and twisted it up with a drill and allen wrench.  I would like a smaller loop on mine but it works.  Really though, a wire with a bent or looped tip so you don't tear the agar is all you need.



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Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
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bw86's Jalisco Grow


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Offlinepillbox
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Registered: 11/13/16
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Last seen: 6 years, 5 months
Re: just starting out [Re: Plazmotech]
    #23851161 - 11/20/16 11:16 AM (7 years, 2 months ago)


does this look right


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Offlinepillbox
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Registered: 11/13/16
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Last seen: 6 years, 5 months
Re: just starting out [Re: pillbox]
    #23851759 - 11/20/16 03:02 PM (7 years, 2 months ago)

transferred from a contaminated dish.


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InvisibleMycolorado
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Re: just starting out [Re: pillbox]
    #23852150 - 11/20/16 05:13 PM (7 years, 2 months ago)

Let it grow out some and take another transfer...still looks funky and might take a few. It's best practice to put transfers in the center of the plate.  Good luck!


--------------------
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Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow


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