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pillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
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Got the print from a friend in another state.
A friend owns a flow hood for his farm. He let me use it for my spores. So was working in front of a hood. Ill give it another go with a syrgine the next time he has time for me to use his hood again.
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 20 minutes
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One print can do a shit load of plates....a little goes a long way
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pillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
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Yes but if the print itself is dirty?
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
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Re: just starting out [Re: pillbox]
#23833084 - 11/14/16 05:40 PM (7 years, 2 months ago) |
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You can clean it up on agar. You should do some reading, it would help. Dick
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
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pillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
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Quote:
A.RichardTrickle said: You can clean it up on agar. You should do some reading, it would help. Dick
lol
Resorting to name calling. Remember barriers tell me to memeber back when I was in preK.
My question is a soild question. If the print is bacterial and I have people telling me not to make a syringe and people having me to make one. I all ready know how to make a sterile syringe and water for it.
The question is for the print.
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 20 minutes
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Re: just starting out [Re: pillbox]
#23833232 - 11/14/16 06:18 PM (7 years, 2 months ago) |
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Most prints are dirty as fuck....that's why we use agar
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pillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
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I am giving my homemade inoculation loop an isopropyl bath right now inside a tall glass with a lid on it.
I got a shotglass, and old syringe. Going to boil the shotglass, take it out wrap in tinfoil and boil again. Use syringe from my farming buddy. I'll need to clean out one of his LC syringes. Going to suck up some boiling water a few times and empty the syringe 4+ times than boil. After that bring it to his flow hood while still in hot water. Wash shotglass with iso, and wash syringe with iso.
Take a few min break for the water to cool. Come back flame sterilize the inoculation loop. Scrape some spores place the loop into the shotglass. Flame sterilize the needle let it cool for a min. Shoot the loop with water to wash off the spores. Agitate the water and than noc up a few petri dishes.
Everything sounds right to me.
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 20 minutes
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Re: just starting out [Re: pillbox]
#23833405 - 11/14/16 07:00 PM (7 years, 2 months ago) |
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Flame it...put a drop of iso on a plate and watch the shit start growing....flaming it is best
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pillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
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Do I use iso on the plate or do I not use iso on the plate.
People say it kills spores and not to use it.
People say use it.
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Re: just starting out [Re: pillbox]
#23833455 - 11/14/16 07:16 PM (7 years, 2 months ago) |
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I had a spore syringe that repetdly produced trich, eventually i was able to isolate clean myc. Probably 5-6 plates later
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Re: just starting out [Re: pillbox]
#23833465 - 11/14/16 07:17 PM (7 years, 2 months ago) |
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Quote:
pillbox said:
Quote:
A.RichardTrickle said: You can clean it up on agar. You should do some reading, it would help. Dick
lol
Resorting to name calling. Remember barriers tell me to memeber back when I was in preK.
My question is a soild question. If the print is bacterial and I have people telling me not to make a syringe and people having me to make one. I all ready know how to make a sterile syringe and water for it.
The question is for the print.
His name is richard, which is often shortened to dick
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
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Re: just starting out [Re: pillbox]
#23833494 - 11/14/16 07:27 PM (7 years, 2 months ago) |
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I was just telling you not to wipe your loop with iso....if you put a drop of iso on a plate it will contaminate.....flame your loop dude
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Leftfield420
bong toker



Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 20 minutes
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Re: just starting out [Re: pillbox]
#23833508 - 11/14/16 07:31 PM (7 years, 2 months ago) |
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If you are wiping your loop with iso I bet that's part of your problem there... I suggest a butane torch lighter....comes in handy when sterilizing needles and such
Edited by Leftfield420 (11/14/16 07:48 PM)
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pillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
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It was sprayed than heat treated with a propane torch. Quote:
Leftfield420 said: Flame it...put a drop of iso on a plate and watch the shit start growing....flaming it is best
That you said put a very small amount on the plate. Made me think of getting some antibotics from a fish store to mix in while agar is cooling.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: just starting out [Re: pillbox]
#23833547 - 11/14/16 07:40 PM (7 years, 2 months ago) |
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Quote:
pillbox said: I am giving my homemade inoculation loop an isopropyl bath right now inside a tall glass with a lid on it.
I got a shotglass, and old syringe. Going to boil the shotglass, take it out wrap in tinfoil and boil again. Use syringe from my farming buddy. I'll need to clean out one of his LC syringes. Going to suck up some boiling water a few times and empty the syringe 4+ times than boil. After that bring it to his flow hood while still in hot water. Wash shotglass with iso, and wash syringe with iso.
Take a few min break for the water to cool. Come back flame sterilize the inoculation loop. Scrape some spores place the loop into the shotglass. Flame sterilize the needle let it cool for a min. Shoot the loop with water to wash off the spores. Agitate the water and than noc up a few petri dishes.
Everything sounds right to me.
This is exactly why I said you'd have serious problems if you made a syringe. On top of that, don't you think if the print is dirty, it'll just make a dirty syringe? Please explain to me the reasoning behind making a syringe to go back to agar? i bet lefty is correct in his guess on the vector. You need to take the alcohol out of the equation.
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pillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
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Well everyone is telling me to make a syringe.
So what do you recommend colo?
That is why I was thinking adding some fish antibiotics while the agar is cooling.
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: just starting out [Re: pillbox]
#23833574 - 11/14/16 07:47 PM (7 years, 2 months ago) |
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Quote:
pillbox said:
Quote:
A.RichardTrickle said: You can clean it up on agar. You should do some reading, it would help. Dick
lol
Resorting to name calling.
hahahahaha I think that's that guy's signature, he always says that at the end of each post
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: just starting out [Re: pillbox]
#23833588 - 11/14/16 07:52 PM (7 years, 2 months ago) |
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Quote:
pillbox said: Well everyone is telling me to make a syringe.
So what do you recommend colo?
That is why I was thinking adding some fish antibiotics while the agar is cooling.
I don't think you need antibiotics. I like making syringes from my spores because personally I find it easier to inoculate plates with a syringe rather than a loop.
Softer agar helps spores germinate quicker, which is a plus. The syringe will also hydrate the spores before, which might make it germinate quicker too. If you get bacteria, and its minor, don't throw away your plate just yet. Wait for the spores to germinate so you can try to get a clean (or relatively clean) wedge. That wedge will grow very rapidly onto that other plate, and in this way you can eventually isolate a clean wedge, and use it to noc a jar.
You can make a syringe if you're absolutely confident you can do so cleanly. If you think you might get even more contams by doing so, avoid it.
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
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Re: just starting out [Re: pillbox]
#23833599 - 11/14/16 07:55 PM (7 years, 2 months ago) |
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Quote:
pillbox said:
Quote:
A.RichardTrickle said: You can clean it up on agar. You should do some reading, it would help. Dick
lol
Resorting to name calling. Remember barriers tell me to memeber back when I was in preK.
My question is a soild question. If the print is bacterial and I have people telling me not to make a syringe and people having me to make one. I all ready know how to make a sterile syringe and water for it.
The question is for the print.
My name, is Dick... It's a play on words son
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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I dont think you should boil the shotglass in water. Try steaming it with tinfoil wrapped around it. If you do that for even 20 minutes at 15 PSI you shouldn't need ISO. Only use ISO if you're too lazy to steam it (which has worked for me, though not great if your print is already really dirty).
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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