Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: < Back | 1 | 2 | 3 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Mycolorado]
    #23832967 - 11/14/16 05:07 PM (7 years, 2 months ago)

Got the print from a friend in another state.

A friend owns a flow hood for his farm. He let me use it for my spores. So was working in front of a hood. Ill give it another go with a syrgine the next time he has time for me to use his hood again.


Extras: Filter Print Post Top
OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 20 minutes
Re: just starting out [Re: Mycolorado]
    #23833014 - 11/14/16 05:21 PM (7 years, 2 months ago)

One print can do a shit load of plates....a little goes a long way


Extras: Filter Print Post Top
Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Leftfield420]
    #23833056 - 11/14/16 05:33 PM (7 years, 2 months ago)

Yes but if the print itself is dirty?


Extras: Filter Print Post Top
OfflineA.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
Re: just starting out [Re: pillbox]
    #23833084 - 11/14/16 05:40 PM (7 years, 2 months ago)

You can clean it up on agar.
You should do some reading, it would help.
Dick


--------------------
"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
"If you're making love to your old lady, someone else is fucking her"
"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


Extras: Filter Print Post Top
Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: A.RichardTrickle]
    #23833212 - 11/14/16 06:15 PM (7 years, 2 months ago)

Quote:

A.RichardTrickle said:
You can clean it up on agar.
You should do some reading, it would help.
Dick





lol

Resorting to name calling. Remember barriers tell me to memeber back when I was in preK.

My question is a soild question. If the print is bacterial and I have people telling me not to make a syringe and people having me to make one. I all ready know how to make a sterile syringe and water for it.

The question is for the print.


Extras: Filter Print Post Top
OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 20 minutes
Re: just starting out [Re: pillbox]
    #23833232 - 11/14/16 06:18 PM (7 years, 2 months ago)

Most prints are dirty as fuck....that's why we use agar


Extras: Filter Print Post Top
Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Leftfield420]
    #23833399 - 11/14/16 06:58 PM (7 years, 2 months ago)

I am giving my homemade inoculation loop an isopropyl bath right now inside a tall glass with a lid on it.

I got a shotglass, and old syringe. Going to boil the shotglass, take it out wrap in tinfoil and boil again. Use syringe from my farming buddy. I'll need to clean out one of his LC syringes. Going to suck up some boiling water a few times and empty the syringe 4+ times than boil.
After that bring it to his flow hood while still in hot water. Wash shotglass with iso, and wash syringe with iso.

Take a few min break for the water to cool. Come back flame sterilize the inoculation loop. Scrape some spores place the loop into the shotglass. Flame sterilize the needle let it cool for a min. Shoot the loop with water to wash off the spores. Agitate the water and than noc up a few petri dishes.

Everything sounds right to me.


Extras: Filter Print Post Top
OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 20 minutes
Re: just starting out [Re: pillbox]
    #23833405 - 11/14/16 07:00 PM (7 years, 2 months ago)

Flame it...put a drop of iso on a plate and watch the shit start growing....flaming it is best


Extras: Filter Print Post Top
Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Leftfield420]
    #23833422 - 11/14/16 07:06 PM (7 years, 2 months ago)

:spun:

Do I use iso on the plate or do I not use iso on the plate.

People say it kills spores and not to use it.

People say use it.


Extras: Filter Print Post Top
Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
Re: just starting out [Re: pillbox]
    #23833455 - 11/14/16 07:16 PM (7 years, 2 months ago)

I had a spore syringe that repetdly produced trich, eventually i was able to isolate clean myc. Probably 5-6 plates later


Extras: Filter Print Post Top
Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
Re: just starting out [Re: pillbox]
    #23833465 - 11/14/16 07:17 PM (7 years, 2 months ago)

Quote:

pillbox said:
Quote:

A.RichardTrickle said:
You can clean it up on agar.
You should do some reading, it would help.
Dick





lol

Resorting to name calling. Remember barriers tell me to memeber back when I was in preK.

My question is a soild question. If the print is bacterial and I have people telling me not to make a syringe and people having me to make one. I all ready know how to make a sterile syringe and water for it.

The question is for the print.




His name is richard, which is often shortened to dick


Extras: Filter Print Post Top
OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 20 minutes
Re: just starting out [Re: pillbox]
    #23833494 - 11/14/16 07:27 PM (7 years, 2 months ago)

I was just telling you not to wipe your loop with iso....if you put a drop of iso on a plate it will contaminate.....flame your loop dude


Extras: Filter Print Post Top
OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 7 hours, 20 minutes
Re: just starting out [Re: pillbox]
    #23833508 - 11/14/16 07:31 PM (7 years, 2 months ago)

If you are wiping your loop with iso I bet that's part of your problem there... I suggest a butane torch lighter....comes in handy when sterilizing needles and such


Edited by Leftfield420 (11/14/16 07:48 PM)


Extras: Filter Print Post Top
Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Leftfield420]
    #23833528 - 11/14/16 07:36 PM (7 years, 2 months ago)

It was sprayed than heat treated with a propane torch.
Quote:




Leftfield420 said:

Flame it...put a drop of iso on a plate and watch the shit start growing....flaming it is best



That you said put a very small amount on the plate. Made me think of getting some antibotics from a fish store to mix in while agar is cooling.


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: just starting out [Re: pillbox]
    #23833547 - 11/14/16 07:40 PM (7 years, 2 months ago)

Quote:

pillbox said:
I am giving my homemade inoculation loop an isopropyl bath right now inside a tall glass with a lid on it.

I got a shotglass, and old syringe. Going to boil the shotglass, take it out wrap in tinfoil and boil again. Use syringe from my farming buddy. I'll need to clean out one of his LC syringes. Going to suck up some boiling water a few times and empty the syringe 4+ times than boil.
After that bring it to his flow hood while still in hot water. Wash shotglass with iso, and wash syringe with iso.

Take a few min break for the water to cool. Come back flame sterilize the inoculation loop. Scrape some spores place the loop into the shotglass. Flame sterilize the needle let it cool for a min. Shoot the loop with water to wash off the spores. Agitate the water and than noc up a few petri dishes.

Everything sounds right to me.



This is exactly why I said you'd have serious problems if you made a syringe.  On top of that, don't you think if the print is dirty, it'll just make a dirty syringe?  Please explain to me the reasoning behind making a syringe to go back to agar?  i bet lefty is correct in his guess on the vector.  You need to take the alcohol out of the equation.


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow


Extras: Filter Print Post Top
Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Mycolorado]
    #23833560 - 11/14/16 07:45 PM (7 years, 2 months ago)

Well everyone is telling me to make a syringe.

So what do you recommend colo?

That is why I was thinking adding some fish antibiotics while the agar is cooling.


Extras: Filter Print Post Top
OfflinePlazmotech
we love the dead
Male


Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
Re: just starting out [Re: pillbox]
    #23833574 - 11/14/16 07:47 PM (7 years, 2 months ago)

Quote:

pillbox said:
Quote:

A.RichardTrickle said:
You can clean it up on agar.
You should do some reading, it would help.
Dick





lol

Resorting to name calling.




hahahahaha I think that's that guy's signature, he always says that at the end of each post


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


Extras: Filter Print Post Top
OfflinePlazmotech
we love the dead
Male


Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
Re: just starting out [Re: pillbox]
    #23833588 - 11/14/16 07:52 PM (7 years, 2 months ago)

Quote:

pillbox said:
Well everyone is telling me to make a syringe.

So what do you recommend colo?

That is why I was thinking adding some fish antibiotics while the agar is cooling.




I don't think you need antibiotics. I like making syringes from my spores because personally I find it easier to inoculate plates with a syringe rather than a loop.

Softer agar helps spores germinate quicker, which is a plus. The syringe will also hydrate the spores before, which might make it germinate quicker too. If you get bacteria, and its minor, don't throw away your plate just yet. Wait for the spores to germinate so you can try to get a clean (or relatively clean) wedge. That wedge will grow very rapidly onto that other plate, and in this way you can eventually isolate a clean wedge, and use it to noc a jar.

You can make a syringe if you're absolutely confident you can do so cleanly. If you think you might get even more contams by doing so, avoid it.


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


Extras: Filter Print Post Top
OfflineA.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
Re: just starting out [Re: pillbox]
    #23833599 - 11/14/16 07:55 PM (7 years, 2 months ago)

Quote:

pillbox said:
Quote:

A.RichardTrickle said:
You can clean it up on agar.
You should do some reading, it would help.
Dick





lol

Resorting to name calling. Remember barriers tell me to memeber back when I was in preK.

My question is a soild question. If the print is bacterial and I have people telling me not to make a syringe and people having me to make one. I all ready know how to make a sterile syringe and water for it.

The question is for the print.



My name, is Dick...
It's a play on words son
:mostinteresting:


--------------------
"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
"If you're making love to your old lady, someone else is fucking her"
"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


Extras: Filter Print Post Top
OfflinePlazmotech
we love the dead
Male


Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
Re: just starting out [Re: Plazmotech]
    #23833600 - 11/14/16 07:55 PM (7 years, 2 months ago)

I dont think you should boil the shotglass in water. Try steaming it with tinfoil wrapped around it. If you do that for even 20 minutes at 15 PSI you shouldn't need ISO. Only use ISO if you're too lazy to steam it (which has worked for me, though not great if your print is already really dirty).


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | Next >  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* bluhoney Start to finish tek bluhoney 5,762 13 04/05/02 10:20 AM
by SouthernGent
* Started my babies on the eve of new years eve! biglo 588 1 01/01/03 05:57 PM
by SixCee
* Re: sterilization w/rubbing alcohol on your syringe Anonymous 2,095 5 01/11/00 05:44 PM
by AnubisRonin
* is this good for a start a kit??? ReeferJ 1,043 6 09/26/01 06:57 PM
by hongus
* Gettin' Started w/ MycoFanatic Kit wan2grow 789 5 12/05/02 09:03 PM
by thepodman
* Getting Started ErikGhint 580 3 02/15/02 07:04 PM
by Mush_Mouth
* no alcohol, can I inoculate mycobags or should I wait? WakeboardrB 748 3 09/02/03 01:35 AM
by Shamanorrhea
* Starting over again Anonymous 450 2 10/07/01 09:52 PM
by FungusFarmer

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
879 topic views. 26 members, 217 guests and 49 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.011 seconds on 15 queries.