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Offlinepillbox
Stranger Danger
Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
just starting out
    #23830642 - 11/13/16 09:39 PM (7 years, 2 months ago)

Got spores in the mail yesterday and made a homemade inoculation loop out of jewelery wire inoculated 4 dishes




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OfflinePlazmotech
we love the dead
Male


Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
Re: just starting out [Re: pillbox]
    #23830866 - 11/13/16 11:22 PM (7 years, 2 months ago)

Dope, good luck! For the record it looks like you have a lot of spores on that dish, probably more than necessary. No need to waste so much. Feel free to post here if you need any help.


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


Edited by Plazmotech (11/13/16 11:22 PM)


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Offlinepillbox
Stranger Danger
Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Plazmotech]
    #23832567 - 11/14/16 03:01 PM (7 years, 2 months ago)

That sucked. Bacteria after 48 hours.

Going to make a syrgine from the print. Any advice before I do?


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OfflineA.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
Re: just starting out [Re: pillbox]
    #23832591 - 11/14/16 03:07 PM (7 years, 2 months ago)

I'd stick with agar....
if you simply must go syringe just use the search and look up "making syringe" check off trusted cultivators and look newer than 5 years.
Good luck
Dick


--------------------
"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
"If you're making love to your old lady, someone else is fucking her"
"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
Re: just starting out [Re: A.RichardTrickle]
    #23832599 - 11/14/16 03:09 PM (7 years, 2 months ago)

You could go print syringe agar and clean it up from there


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OfflinePlazmotech
we love the dead
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Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
Re: just starting out [Re: A.RichardTrickle]
    #23832618 - 11/14/16 03:15 PM (7 years, 2 months ago)

I've inoculated a few agar dishes with spore syringes too. To make a syringe it's pretty easy.

Follow the Sterile syringe tek. Put the sterile syringe after its cooled down into your still air box (SAB). An SAB is just a box with holes in it for arms. Put a shot glass and your print in there, too. Spray the inside with Lysol, and let sit for 5-10 minutes. Put on some latex gloves, alcohol them. Alcohol the outside of your syringe, and wipe down the entire shot glass with alcohol. Flame sterilize the needle. Scrape some spores from the print into the shot glass using a scalpel or whatever (make sure to flame sterilize). Spray the water from the syringe into it, then aspirate. Spray out again, and do this a few times to break up the spores. Finally aspirate it again and cap.

If you need more detailed instructions I can provide them later today. I'm on mobile right now, so it's irritating to type too much.


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


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Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: numnum59]
    #23832620 - 11/14/16 03:16 PM (7 years, 2 months ago)

The bacteria was just around the place I used the loop on. I Presser cooked the loop I used and flame sterilized before using. I cooled it in the agar before scrapping off some spores.
Treating the spores with alcohol a good idea?
Syringe to agar is the plan


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: just starting out [Re: pillbox]
    #23832639 - 11/14/16 03:22 PM (7 years, 2 months ago)

Quote:

Treating the spores with alcohol a good idea?



no

click the link in my signature, then click the agar link, then click the first one there.

you can find my pictures of how I put spores on to agar.


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InvisibleMycolorado
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Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: just starting out [Re: pillbox]
    #23832661 - 11/14/16 03:29 PM (7 years, 2 months ago)

Quote:

pillbox said:
The bacteria was just around the place I used the loop on. I Presser cooked the loop I used and flame sterilized before using. I cooled it in the agar before scrapping off some spores.
Treating the spores with alcohol a good idea?
Syringe to agar is the plan



You don't need to PC a loop, just flame sterilize.  Anyway, if you're having issues with bacteria going from print to plate, you're going to have major bacteria/contamination issues making a syringe and then going to plate.  The whole idea is just silly to me...why introduce more vectors for contamination?  You might try to post up a decent pic of your plate with contamination...it might not be totally toast...


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Helpful Threads
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Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Mycolorado]
    #23832717 - 11/14/16 03:46 PM (7 years, 2 months ago)

Delete


Edited by pillbox (11/14/16 03:47 PM)


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Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Mycolorado]
    #23832719 - 11/14/16 03:46 PM (7 years, 2 months ago)







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OfflinePlazmotech
we love the dead
Male


Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
Re: just starting out [Re: pillbox]
    #23832730 - 11/14/16 03:51 PM (7 years, 2 months ago)

Quote:

pillbox said:
Treating the spores with alcohol a good idea?




No this kills the spore.


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


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Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Plazmotech]
    #23832748 - 11/14/16 03:57 PM (7 years, 2 months ago)

4 dishes and no spores. None of those have bacteria..


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OfflinePlazmotech
we love the dead
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Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
Re: just starting out [Re: pillbox]
    #23832756 - 11/14/16 04:00 PM (7 years, 2 months ago)

Sorry, what do you mean?


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


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Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Plazmotech]
    #23832764 - 11/14/16 04:03 PM (7 years, 2 months ago)

I have 4 control dishes to make sure it was not my agar prep that failed.


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OfflineLeftfield420
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Registered: 02/26/16
Posts: 10,023
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Re: just starting out [Re: pillbox]
    #23832778 - 11/14/16 04:08 PM (7 years, 2 months ago)

At first it was hard for me to get my sterile technique down...you gotta make sure your hand is not above agar when working..keep at it, you'll be much happier with results and save materials in the long run...


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
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Re: just starting out [Re: Leftfield420]
    #23832793 - 11/14/16 04:13 PM (7 years, 2 months ago)

Knoxj up more plates to start


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineLeftfield420
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Registered: 02/26/16
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Re: just starting out [Re: cronicr]
    #23832806 - 11/14/16 04:16 PM (7 years, 2 months ago)

I agree with Cronicr inoplates more plates, you'll get results eventually....


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Offlinepillbox
Stranger Danger

Registered: 11/13/16
Posts: 33
Last seen: 6 years, 5 months
Re: just starting out [Re: Leftfield420]
    #23832839 - 11/14/16 04:25 PM (7 years, 2 months ago)

Maybe. Depends how far this print will last before it becomes useless


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InvisibleMycolorado
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Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: just starting out [Re: pillbox]
    #23832936 - 11/14/16 04:55 PM (7 years, 2 months ago)

Yikes...definitely looks like it came in with the spores.  Mind describing what exactly you're doing and where the print came from?  Would appear to be super dirty.


--------------------
๐Ÿ…‚๐Ÿ„ธ๐Ÿ„ถ๐Ÿ„ผ๐Ÿ„ฐ


Helpful Threads
stonesun
Mycoloradoโ€™s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow


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