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OfflineAgarStudent
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Panaelous (Coperlandia Cyanescens - Australia)
    #23830162 - 11/13/16 07:03 PM (7 years, 2 months ago)

Hi Guys –

Having looked at lots of pan cyan teks, RR’s notes and trying to pick out the best of it all, this is my first attempt and was just wondering if the process so far looks legit?

1) Colonised rye berries of Coperlandia Cyanescens



2) Using a spawn ratio of about 50/50, mixed with pasteurised hpoo ( my substrate is 10 parts hpoo, 1 part gypsum, 1 part vermiculite as per RR instruction).
3) Cased (less than half an inch thick) with pasturied (10 parts peat moss, 1 part gypsum, 1 part calcium carbonate as per RR instruction)
4) Small trays 5 x 10 x 3 inches high used



5) Will leave to colonise these for 4-5 days at about room temperature (18-20 Deg C)
6) Will place three trays in SGFC and placed under 6500K light for 12 hours/day.



Can anyone please let me know if any processes are lacking or if the process appears okay?
Much thanks :smile:


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InvisibleSporeDaddy
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23830397 - 11/13/16 08:15 PM (7 years, 2 months ago)

Seems good on part of having the right materials/lighting. I am also starting my first Panaeolus Cyan. grow. My research shows:

1. Inoculate agar and should colonize in 72 hours at 80-85 F.

2. Wedges of agar into rye grain, at least two wedges for a quart to increase speed. Keep temp at 80-85 F for best growth in this stage.

3. Break apart colonized rye grain and spread over your manure that you placed in your tray, like 1/2-1 inch deep, then place an equal amount on top of the rye grain. Allow to colonize at same temps. in dark, humid, high CO2 environment. Should break through on surface with patches of mycelium and proceed to colonize.

4. Once your surface is 75% covered use your casing to cover the entire surface and mist till moist to the eye. And place into fruiting conditions with airflow and light. Plus decrease temp. to like 72-75F

This is what I have gathered so far on the entire process using a monotub/SCFC, it may not be 100% :dumbhunter:


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"Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom."
- Terrence McKenna

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Edited by SporeDaddy (11/13/16 08:17 PM)


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OfflineAgarStudent
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: SporeDaddy]
    #23830540 - 11/13/16 08:59 PM (7 years, 2 months ago)

Thanks for that.

Feel free to keep us updated on your progress.  Did you end up using a multispore or an isolate?


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InvisibleSporeDaddy
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23832302 - 11/14/16 01:47 PM (7 years, 2 months ago)

I chose to quickly inoculate the agar with multi-spore spots in the center, though i could have done isolates using my microscope. I usually do that with second generation spore prints after using the primary spore syringe, just something i prefer to do.


--------------------
"Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom."
- Terrence McKenna

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OfflinePanarchist
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23833463 - 11/14/16 07:17 PM (7 years, 2 months ago)

Quote:

AgarStudent said:


Can anyone please let me know if any processes are lacking or if the process appears okay?
Much thanks :smile:




looks like your on the right track there's just some slight modifications you might want to consider as the thinking on pans has evolved a little since RR wrote his teks. if you've got clean spawn you can easily increase that spawn/substrate ratio to 1:2. and many pan growers use a substrate mix that includes straw or coir because manure with just verm can get a bit muddy. most mix equal parts of manure and straw/coir. and those trays should be fine just keep your substrate no more than 2 inches deep. and a number of growers have found that giving your substrate a few extra days to consolidate before casing and introducing to fruiting conditions helps to prevent overlay of the casing layer which will hinder the mycelium's ability to put out fruits. if using a 1:2 spawn/substrate ration you would then expect full visible colonization at around 7 days and then you would want to give at least 3 days after that before it's ready to fruit.


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OfflineAgarStudent
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: Panarchist]
    #23834395 - 11/15/16 03:05 AM (7 years, 2 months ago)

Thanks for that.  Can you see any real issue in casing at the same time I did the spawning?
Next time I will try adding straw and/or coco.  Is poo, verm, gypsum still a fair substrate or does the poo/straw/coco beat that?  Does that also have gypsum, vermiculite?


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Why reinvent the wheel when someone here has already done the science?



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InvisibleSporeDaddy
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23834598 - 11/15/16 07:20 AM (7 years, 2 months ago)

manure, vermiculite and gypsum are a good combination that provide nutrients for growing mycelium especially in Pan. Cyan. Yet it seems to increase the quality of your fruiting stage when you have a mix of coir or straw added with manure and verm.


--------------------
"Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom."
- Terrence McKenna

:nicetry::heytherebadboy:


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OfflinePanarchist
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23835518 - 11/15/16 01:32 PM (7 years, 2 months ago)

Quote:

AgarStudent said:
Thanks for that.  Can you see any real issue in casing at the same time I did the spawning?
Next time I will try adding straw and/or coco.  Is poo, verm, gypsum still a fair substrate or does the poo/straw/coco beat that?  Does that also have gypsum, vermiculite?






the problem that can happen from casing early is that the mycelium will sometimes start to consume the casing layer and form these blobs that won't fruit. it seems like when you give the mycelium time to consolidate it switches from being in a digestive mode into a fruiting mode. it isn't always a problem but i've seen a bunch of growers have issues with it. and adding straw or coir to your manure helps give the substrate a fluffier consistency that makes it easier for the mycelium to colonize it. it also increases the substrates capacity for water retention. you should probably do alright with what you've got going though. and it really comes down to personal choice as to what other additions you may want to add to the substrate. some add verm and or gypsum but others do not. in my opinion, since vermiculite is nonnutritive it offers no benefit but i could be wrong. gypsum may be beneficial but i have seen some who suggest that it isn't really.


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OfflineAgarStudent
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: Panarchist]
    #23837405 - 11/16/16 12:02 AM (7 years, 2 months ago)

Thanks for the help :smile:

I have given then trays about 3-4 days to colonise and this is what I have now:



Does this look ready to fruit?
Do they have to be cold-shocked, put in the fridge or any weird s**t like that?
Dumb question, but IMHO there too many mixed ideas on the topic and I am confused.

Cheers


Edited by AgarStudent (11/16/16 12:17 AM)


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InvisibleSporeDaddy
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23838129 - 11/16/16 08:59 AM (7 years, 2 months ago)

Looks pretty well colonized with primordial growth, fruiting could be initiated at your current point id say. If you got an casing I would definitely use on your copelandia. Just place a very thin layer covering the top surface only a cm or two deep, just enough to cover the mycelium. The casing will trap in humidity in the soil while fruiting but no one said you couldn't fruit without it. As soon as casing is on or go ahead and put into FC
:putin:


--------------------
"Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom."
- Terrence McKenna

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OfflinePanarchist
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: SporeDaddy]
    #23838399 - 11/16/16 10:47 AM (7 years, 2 months ago)

i deleted a comment i made in this thread because i failed to make a key observation relating to where you're at in the process before making it. it had some useful information in it but it didn't quite apply to your current situation. if you have already cased it then you gotta start fruiting it. it looks like there may be some signs of overlay taking place but hopefully it does not become problematic. and don't expose panaeolus mycelium to any kind of cold or even cool conditions. temperatures below 70F/21C will cause the mycelial growth to slow down and temperatures below 60F/15C can permanently stall it.


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OfflineAgarStudent
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: Panarchist]
    #23839578 - 11/16/16 05:29 PM (7 years, 2 months ago)

They are pinning now thanks guys!  Should I must then just yet?


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InvisibleComebackKid
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23839599 - 11/16/16 05:36 PM (7 years, 2 months ago)

:popcorn:


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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: ComebackKid]
    #23839622 - 11/16/16 05:44 PM (7 years, 2 months ago)



Sorry the camera is a bit shit.  That is a yes to misting? :smile:


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OfflinePanarchist
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23839744 - 11/16/16 06:42 PM (7 years, 2 months ago)

no, i think ComebackKid was just saying that he's watching the thread. if the pinset is widespread you should stop misting altogether. if it's just in small areas you can still mist where there's not pins popping up yet but be careful not to get any on the pins as it will cause aborts. it's probably not even necessary unless the surface of the casing layer looks like it's getting dry.


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InvisibleComebackKid
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: Panarchist]
    #23839887 - 11/16/16 07:40 PM (7 years, 2 months ago)

Just watching. I litterally just put cyans to agar an hour ago


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineAgarStudent
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: ComebackKid]
    #23840081 - 11/16/16 09:11 PM (7 years, 2 months ago)

I am just a bit concerned about the misting:

Quote:

RogerRabbit said:
Quote:

is it necessary to mist the cakes before each fanning/air exchange (2-3 times per day),




Yes, you'll need to mist a few times daily for best performance.  A good rule of thumb is if you're getting the proper amount of fresh air exchange for maximum pinning and yield, your substrates will need misting three or more times daily.
RR




This is the SGFC, it is pretty packed, but do you think the trays will have enough air exchange and humidity, or do I need a second chamber?



Does this substrate look dry?  I live in a dry climate, would the same rule apply as per cubes where you always want to see droplets on the tray.  I never dunked it or anything:



Here is another shot - Not sure if cyans are meant to be this dry, but I hope to write up a tek if the process works:



--------------------
Why reinvent the wheel when someone here has already done the science?



Edited by AgarStudent (11/17/16 06:27 AM)


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OfflinePanarchist
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23841835 - 11/17/16 01:49 PM (7 years, 2 months ago)

i could be wrong but i think you're a bit overcrowded in that SGFC. it looks like two trays would be more optimal in that size bin. and that substrate looks dry. you don't want droplets so much, more like just a layer of moisture enough so that it glistens. if you didn't dunk your grainspawn your combined substrate may be lacking in water at this point. it may be advisable to give a heavy misting to boost those hydration levels back up. then keep it moist until pins start to form.


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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: AgarStudent]
    #23841918 - 11/17/16 02:12 PM (7 years, 2 months ago)

I'm pretty sure it's Copelandia not Coperlandia, right?


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InvisibleSporeDaddy
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: Plazmotech]
    #23841941 - 11/17/16 02:18 PM (7 years, 2 months ago)

:whathesaid:

It is Copelandia, he just misspelled lol:popcorn:


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"Mushroom by nature is among the most powerful machines created by nature, people are the only thing to rival the mushroom."
- Terrence McKenna

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OfflineAgarStudent
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Re: Panaelous (Coperlandia Cyanescens - Australia) [Re: SporeDaddy]
    #23842711 - 11/17/16 05:29 PM (7 years, 2 months ago)

Thanks for the feedback :wink:



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Why reinvent the wheel when someone here has already done the science?



Edited by AgarStudent (11/21/16 12:21 AM)


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