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Offlinefrankwhite85
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grains too dry for agar
    #23829342 - 11/13/16 02:06 PM (7 years, 2 months ago)

So I cooked up a round of jars for some agar to grain.  I pc'd them in a 75X AA sterilizer but I forgot to check the pressure plug beforehand.  If i don't check them each time to make sure they are flush they can leak pressure.  So it cooked fine but when i went to release the pressure valve in the morning no air came out so the pressure definitely leaked out over night.  It looked like it sucked most of the moisture out of the grains but i put some ms agar wedges to them anyway.  After a week it really didn't look like the agar had grown into the grain all that much.  Anybody ever have this problem?


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InvisiblebodhisattaMDiscordReddit
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Re: grains too dry for agar [Re: frankwhite85]
    #23829370 - 11/13/16 02:20 PM (7 years, 2 months ago)

if anything the grains come up to hydration in a sterilizer. the inside is filled with steam, it's not an oven. it doesn't dry anything out. air gets sucked in when it cools unless you use a sterilizer properly it holds a vacuum til you break it. useful for opening in front of a flow hood if you have sterilized things without filter lids, like an Erlenmeyer flask with a foil lid only. though that's a ultra rare contamination scenario anyway.

you certainly can burn or caramelize your grain though which hinders performance. pictures would be more telling


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OfflineKenetic
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Re: grains too dry for agar [Re: frankwhite85]
    #23829378 - 11/13/16 02:26 PM (7 years, 2 months ago)

I'm not being a smartass but it sounds like you don't know how a pressure cooker operates?  Of course it "leaked" out the pressure overnight.  How do you expect to open it if it never loses pressure? 

It could be me too, I'm not sure if AA's operate differently than other pc's besides the way they close. 

As far as your grains drying or looking dry, you haven't specified what type of lids you're using, grain prep, how long you ran the cycle etc.

Edit:  Apparently they are different.  Obviously I don't know how that sterilizer works lol


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InvisibleSporeDaddy
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Re: grains too dry for agar [Re: Kenetic]
    #23829402 - 11/13/16 02:33 PM (7 years, 2 months ago)

:whathesaid:

Only reason for dry grain after sterilization in pressure cooking types would be you probably did not mix enough water with the grain to absorb and dried through the night


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OfflineKenetic
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Re: grains too dry for agar [Re: SporeDaddy]
    #23829409 - 11/13/16 02:34 PM (7 years, 2 months ago)

I didn't say that lol


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Offlinefrankwhite85
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Re: grains too dry for agar [Re: Kenetic]
    #23829413 - 11/13/16 02:36 PM (7 years, 2 months ago)

When a sterilizer works properly you crack the vent once its cooled and pressurized air releases for 5-10 seconds.  When the pressure plug is not flush there was literally no air that came out when i cracked it because it leaked.  I use a mix of Bob's red mill rye berries and white sorghum.  I wash thoroughly then let it soak for 12 hours with gypsum and then do about a 30 min simmer then strain.  The grains just looked drier than usual after cooking I was just wondering if this could be the reason the culture didn't really take off into the grains.


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OfflineKenetic
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Re: grains too dry for agar [Re: frankwhite85]
    #23829433 - 11/13/16 02:41 PM (7 years, 2 months ago)

Nice.  I learned something too.  With what you just told me about how it works, I doubt that would dry your grains out.  I don't see how it would anyway.

I'm not sure what your experience is with agar to grain but in my experience things always start off a little slow until I shake it up.


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Re: grains too dry for agar [Re: frankwhite85]
    #23829443 - 11/13/16 02:45 PM (7 years, 2 months ago)

could be your agar slice was not good or even your temps. are off for incubation. Definitely could be multitude of factors.


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OfflineThe Mycologist
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Re: grains too dry for agar [Re: Kenetic]
    #23829449 - 11/13/16 02:47 PM (7 years, 2 months ago)

IDK my pressure cooker behaves like kenetic's. The pressure comes from water changing states, so when it cools the pressure dissipates.


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