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Offlinemaestro50
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agar to brf
    #23827907 - 11/13/16 04:13 AM (7 years, 2 months ago)

Hello my peeps!!

So ive been transfering agar for some time now and ive recently transfered it to BRF cake but im concerned about top vermiculite layer... Ive put it on the verm layer.. will it work still?


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Re: agar to brf [Re: maestro50]
    #23827915 - 11/13/16 04:18 AM (7 years, 2 months ago)

Ive never done brf but I dont think it will based on the fact thats its purpose is basically a filter..

But you should do agar->grain


Edited by spore-ty (11/13/16 04:19 AM)


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Invisibleblindingleaf
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Re: agar to brf [Re: maestro50] * 1
    #23827916 - 11/13/16 04:18 AM (7 years, 2 months ago)

if its thick (and dry like it should be) i would say probably not.  if u have open holes on ur lid, as per the standard PF TEK, then chances are even more slim

just use a grain jar lid (filtered GE hole) and no verm layer, and it will definitely work.


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Offlinespore-ty
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Re: agar to brf [Re: blindingleaf]
    #23827917 - 11/13/16 04:21 AM (7 years, 2 months ago)

Haha nice :whathesaid:


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Offlinemaestro50
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Re: agar to brf [Re: blindingleaf]
    #23828065 - 11/13/16 06:31 AM (7 years, 2 months ago)

Quote:

blindingleaf said:
if its thick (and dry like it should be) i would say probably not.  if u have open holes on ur lid, as per the standard PF TEK, then chances are even more slim

just use a grain jar lid (filtered GE hole) and no verm layer, and it will definitely work.




i have about half inch of verm and glass with alu foil. Should i try to transfer agar deeper into the BRF mix?


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Invisibleblindingleaf
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Re: agar to brf [Re: maestro50]
    #23828083 - 11/13/16 06:42 AM (7 years, 2 months ago)

ummm  maybe?  if u do that, worst that will happen is mold will hit the uncolonized parts of the agar and grow from there, or ride in while ur digging. if that happens, it should be obvious within a week that its contaminated.

i would start over personally.  use real lids, like metal or plastic.  even salsa or jam jars and their lids.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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OfflinePinPornProducer
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Re: agar to brf [Re: maestro50]
    #23828115 - 11/13/16 07:12 AM (7 years, 2 months ago)

If I was to go agar to brf I would take a syringe full of sterile water and squirt the water into the agar plate to dislodge the myc and then suck it back up into the syringe. Then inoculate the brf jars normally :shrug:


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OfflineNDStepp84
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Re: agar to brf [Re: PinPornProducer]
    #23828159 - 11/13/16 07:37 AM (7 years, 2 months ago)

Yeah that or a blenderless LI and use a syringe to inject the LI just like you would spore solution.


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InvisibleMad Season
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Re: agar to brf [Re: NDStepp84]
    #23828207 - 11/13/16 08:08 AM (7 years, 2 months ago)

Yep that's what I'd do too. LI to pftek


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Invisibleblindingleaf
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Re: agar to brf [Re: Mad Season]
    #23828265 - 11/13/16 08:37 AM (7 years, 2 months ago)

thats a good call, didn't think about that


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Offlinemaestro50
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Re: agar to brf [Re: blindingleaf]
    #23828321 - 11/13/16 08:59 AM (7 years, 2 months ago)

ive spend months cleaning agar so i would like to keep my mycelium... and i dont have more spores... whats the best thing i can do now? Isnt agar to cake normal way to do it?

Maybe it will penetrate and develop and everything will be ok?


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Offlineenlightenment
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Re: agar to brf [Re: maestro50]
    #23828324 - 11/13/16 09:01 AM (7 years, 2 months ago)

Agar to grain is normal. Agar to cake is not practical because of the dry vermiculite layer (if you use PF tek).
As the others said you can make an LI or LC and inoculate with this. you can poke through the dry verm layer with a syringe.

Why don't you use grain? Do you have a PC? It seems like you have one because of the agar. Better to work with grain than BRF cakes IMO.


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Offlinespacechildo
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Re: agar to brf [Re: maestro50]
    #23828620 - 11/13/16 10:38 AM (7 years, 2 months ago)

Quote:

maestro50 said:
ive spend months cleaning agar so i would like to keep my mycelium... and i dont have more spores... whats the best thing i can do now? Isnt agar to cake normal way to do it?

Maybe it will penetrate and develop and everything will be ok?




myc wont grow through dry verm, agar is pretty much pointless with pf-tek IMO, pf-tek is fool proof enough as it is so just use spore syringes.


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Offlinemaestro50
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Re: agar to brf [Re: spacechildo]
    #23829797 - 11/13/16 04:48 PM (7 years, 2 months ago)

Ok guys im asking u can i save it or not??

Should i take my cake and do something with it? Ive cleaned this culture so much. Dont tell me to make it again because i know that i can make it again. How can i try to save cake?

Im learning still so. And i have PC


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Offlinespacechildo
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Re: agar to brf [Re: maestro50]
    #23829854 - 11/13/16 05:09 PM (7 years, 2 months ago)

buy a bag of grains and go back to your plates for more clean myc.


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InvisibleJosex
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Re: agar to brf [Re: spacechildo]
    #23830146 - 11/13/16 06:58 PM (7 years, 2 months ago)

If I were to do this I'd use quart pp5 plastic jars with wide mouth and filtered lids. Fill the bottles a little less than half with the PF mix and don't add the dry verm layer. Then use your agar culture to make an LI and pour it into the bottles.

Then you can choose between growing the cakes invitro in the bottles or taking them out of the bottles to fruit them normally in a SGFC.


Edited by Josex (11/13/16 07:04 PM)


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