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OfflineKillaloting
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Registered: 06/03/14
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Re: No PC? No worries! Lazy tek [Re: Kenetic]
    #23827988 - 11/13/16 05:37 AM (7 years, 2 months ago)

I suggest you tell santa your mother does not want him to visit this year, since you been a bad boy.


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InvisibleMad Season
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Re: No PC? No worries! Lazy tek [Re: Killaloting]
    #23828000 - 11/13/16 05:46 AM (7 years, 2 months ago)

Quote:

Killaloting said:
it looks alot better then ur petri dishes thats for sure. pure thick white fluffy all over, no wet spots nothing.



My contam art is beautiful :P. Thick eh? That's what I use to explain mycelial bacterial reactions...

Oh and do you really want me to pull RR quotes and shit? 90 minutes of boiling grains doesn't work if you want 100% success. Your measly 100 jars is nothing on RRs 20000+

Quote:

RogerRabbit said:
Quote:

UnnamedGrower said:

Steam sterilization doenst have enough penetration power to get into the heart of the grain and kill all the contams hidden within it. 15 PSI for 1.5 hours the pressure built up within the PC gets into the heart and kills the contams.  BRF on verm everything is exposed the steam has contact with everything and kills all the contams




This is incorrect.

Pressure doesn't penetrate, because the jars or bags are at the same pressure.  The pressure simply allows the boiling point to raise, thus making the steam hotter.

You're also incorrect that steam sterilization does not work for grains.  I live in the mountains where water boils well below 212F/100C and I get 100% success with 7 pound rye berry bags, steam-sterilized at atmospheric pressure for 8 hours.

One can use a pressure sterilizer for a shorter amount of time, or atmospheric sterilization for a longer time.  8 hours gives me one hundred percent success for supplemented sawdust, as well as grains.  The proof of this is zero contamination in the last +/- 20,000 substrate blocks done this way.
RR



Also this method has been practiced and attempted to be proven wrong so much over the course of 15 years. Everyone now unanimously agrees 8 hours is needed for 100% success. The people who skimped out on it eventually found they either need a PC or go for 8 hours. You may think otherwise, but it's just a matter of time.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
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Offlinespore-ty
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Re: No PC? No worries! Lazy tek [Re: Killaloting] * 1
    #23828003 - 11/13/16 05:48 AM (7 years, 2 months ago)

Lmao his last remark made me chuckle uncontrollably

Quote:

Killaloting said:
it looks alot better then ur petri dishes thats for sure. pure thick white fluffy all over, no wet spots nothing.




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OfflinePinPornProducer
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Re: No PC? No worries! Lazy tek [Re: spore-ty]
    #23828009 - 11/13/16 05:53 AM (7 years, 2 months ago)



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OfflineX2QE
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Re: No PC? No worries! Lazy tek [Re: Killaloting]
    #23828011 - 11/13/16 05:55 AM (7 years, 2 months ago)

Umm for most people on this forum this is a hobby.

WTF am I looking at in the second picture?


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OfflinePinPornProducer
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Re: No PC? No worries! Lazy tek [Re: Killaloting]
    #23828014 - 11/13/16 05:57 AM (7 years, 2 months ago)

Quote:

Killaloting said:
As stated like 100 times this isnt for veteran users, so you all veteran users can take ur veteran badge and go read other methods as you have more and better equippment and willingly and able to spend more time in this "hobby".

TBH I think your guys attitudes agains newer people is what mods are NOT looking for but let them answer that themselves.

Here is my very latest project this is used coffea with filter and everything microwaved only, the lids isnt even a tight seal and no contams yet and growt is starting now already in day 2.





On picture 2 there is a few normal rice in middle of it, the plan is just to see if they start to mold when placed there because i know for a fact that if I placed them ontop they would be.


To clearify, this post is not intended for any "veteran" user, there is way more failproof methods as said in the thread already and if your veteran your probably already have ur isolate and petridishes and whatever, this is a:

"SIMPLE CHEAP "BUSH-STYLE-DIY-METHOD" THAT WILL PRODUCE MUSHROOMS, IN SOME CASES YOU MIGHT GET CONTAMINATED AND THERE ARE FAR MOR ADVANCED METHODS WITH BETTER SUCCES-RATIO BUT THIS HAS BEEN FAILPROOF FOR ME!"


Now stop the of topics and spam, serious questions input will be answered. others will be reported.



:goodluckwiththat2:

Such a lazy tek that you can't even remove the labels off the jars? How do you know what behind the labels are clean?

Pretty sure those are going to be a bacterial mess


Edited by PinPornProducer (11/13/16 06:09 AM)


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OfflineKillaloting
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Re: No PC? No worries! Lazy tek [Re: Mad Season]
    #23828025 - 11/13/16 06:02 AM (7 years, 2 months ago)

you have made close to 10.000 posts but have you ever read one?

obviously not, go back and read what it says and then u can edit your reply so you at least appear to be one of the more educated enoght to read posts before you reply to them.

Also you know very little about sterilisation, its all about temperature, but again, i did not say its sterile its just sterile enoght for a live culture to get a good havest, obviously.


There is not much difference in putting something at 120C in the oven or 120C in ur PC its still all about getting the substrade to the correct temperature for the correct amounth of time, to be 1000% sterile you might need 24h even but that is not necesary im saying.


"Hot air is among the most efficient sterilisationsmethod available, because it seems to sporefree and sporecarrying microorganisms.
Superficial sporfree bacteria is killed at 90 ° C - 95 ° C within 1/4 hour, if the air has a moisture content of 60% relative humidity. Pests and its eggs are killed by 60 ° C hot air of 30 min.
Streaming steam of 100 ° C killing anthrax spores, typically within 5 minutes, sporfria bacteria in 1 minute. Boiling water of 100 ° C have approximately the same effect as the flowing steam of the same temperature. Sporfria bacteria, including some pathogens, killed by heating in water, milk or similar liquids at 65 ° C, for 15-30 minutes at 80 ° C within one minute. The process wherein sporfria killed bacteria in milk, called pasteurization. Another way of sterilizing is using an autoclave where the object to be sterilized is placed in a pressure chamber. Saturated steam enters under pressure, heating of the estate is done by the steam releases its heat energy to the surfaces on which it condenses, this condensation continues until the entire cargo mass reached steam own temperature, such as 134 ° C for 3 min, and kill every living including spores in the container."


translated from swedish wikipedia


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OfflineKillaloting
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Re: No PC? No worries! Lazy tek [Re: X2QE]
    #23828034 - 11/13/16 06:08 AM (7 years, 2 months ago)

Quote:

X2QE said:
Umm for most people on this forum this is a hobby.

WTF am I looking at in the second picture?




hehe it is an experiment, i have made 2 test spawns of used coffea grounds with the filters everything just put in jars, microwaved for 2x2 min and then added culture.

Ive added a few just normal boiled rice in the middle of 1 just to se how it affects the growt but also perhaps yield, i do suspect I will get that one contaminated, im kinda sure but so far so good, but I have only bad experience using thoose kinds of substrades that sticks to eachother etc its just not good, but i know for a fact that if they were placed ontop they would be mold magnets, maybe in the middle of everything they wont contaminate the shit i dont know its experimental, i do this everyday, different experiments :smile:

I have even grown on toilet paper roll dunked in boiling liquid coffea with great succes.

as you say its a hobby :smile:


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InvisiblePastywhyteMDiscord
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Re: No PC? No worries! Lazy tek [Re: Killaloting]
    #23828042 - 11/13/16 06:13 AM (7 years, 2 months ago)

Do you have any pics of mushrooms grown with this method?


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Invisibleblindingleaf
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Re: No PC? No worries! Lazy tek [Re: Killaloting]
    #23828044 - 11/13/16 06:13 AM (7 years, 2 months ago)

if u would just put some pictures up of ur process and pictures of the results, it would be a lot less easy to underhand you.

in my minds eye when I stretch my imagination to its fullest limit, I could see how there is a slim slim chance that boiled grains and SUPER sterile technique could work in a short timeframe, but u'd need a heavy live mycelial inoc rate and a very clean place, and I still think the chances would be super slim, and I wouldn't count it as a success unless I saw fruits

edit:pasty beat me


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InvisibleMad Season
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Re: No PC? No worries! Lazy tek [Re: Killaloting]
    #23828055 - 11/13/16 06:23 AM (7 years, 2 months ago)

Quote:

Killaloting said:
you have made close to 10.000 posts but have you ever read one?

obviously not, go back and read what it says and then u can edit your reply so you at least appear to be one of the more educated enoght to read posts before you reply to them.

Also you know very little about sterilisation, its all about temperature, but again, i did not say its sterile its just sterile enoght for a live culture to get a good havest, obviously.


There is not much difference in putting something at 120C in the oven or 120C in ur PC its still all about getting the substrade to the correct temperature for the correct amounth of time, to be 1000% sterile you might need 24h even but that is not necesary im saying.


"Hot air is among the most efficient sterilisationsmethod available, because it seems to sporefree and sporecarrying microorganisms.
Superficial sporfree bacteria is killed at 90 ° C - 95 ° C within 1/4 hour, if the air has a moisture content of 60% relative humidity. Pests and its eggs are killed by 60 ° C hot air of 30 min.
Streaming steam of 100 ° C killing anthrax spores, typically within 5 minutes, sporfria bacteria in 1 minute. Boiling water of 100 ° C have approximately the same effect as the flowing steam of the same temperature. Sporfria bacteria, including some pathogens, killed by heating in water, milk or similar liquids at 65 ° C, for 15-30 minutes at 80 ° C within one minute. The process wherein sporfria killed bacteria in milk, called pasteurization. Another way of sterilizing is using an autoclave where the object to be sterilized is placed in a pressure chamber. Saturated steam enters under pressure, heating of the estate is done by the steam releases its heat energy to the surfaces on which it condenses, this condensation continues until the entire cargo mass reached steam own temperature, such as 134 ° C for 3 min, and kill every living including spores in the container."


translated from swedish wikipedia



So none of those things are even talking about endospores or grain media... Grain media is filled with air pockets that act as insulation from heat.. So literally none of those examples work. That's why you need to have it boiled for 8 hours, and can boil liquid media to sterility in an hour tops.

So if it isn't about having sterile grains, why is the most important aspect of this hobby clean spawn?


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinePinPornProducer
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Re: No PC? No worries! Lazy tek [Re: blindingleaf] * 1
    #23828056 - 11/13/16 06:23 AM (7 years, 2 months ago)

I tried this method last night :super:



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OfflineKillaloting
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Re: No PC? No worries! Lazy tek [Re: blindingleaf]
    #23828057 - 11/13/16 06:25 AM (7 years, 2 months ago)

Since ur polite enoght to not include childish accusations or curse words ima give you an honest reply, yes there has been fruits, there will also be more fruits.

Mycilium growth in my country is legal but not all fruits from it is... how do you guys solve this? Or is it just a risk your willing to take?


And your right this wont be 100% failproof forever, that would just be redicoulus but that ive had this much succes so far, means that it can be worth for someone to try that maybe never will try if it means investing in to much stuff, thats the whole point that people here doesnt understand, Like i can tell you the best way how to grow stuff and it will cost you 100.000 us to start it or i can suggest another way for small timers that has no serious interest ( at least yet ) so they try and get more interested and therefore perhaps advance, this isnt posted in advanced mycology thread ,its clearly stated its not a bulletproof sterilisation method, all its saying is how i have done, and that it works for me.

Why dont you go ahead and try it on 1 jar? And place your input here so can you help silence the haters :smile:

It will cost you roughly 40 cents, i can send u a few prints for the hustle :smile:


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InvisibleMad Season
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Re: No PC? No worries! Lazy tek [Re: PinPornProducer]
    #23828058 - 11/13/16 06:25 AM (7 years, 2 months ago)



A jar he sent me in PM for everyone to see. I mean it's not the worst jar, but thick, lumpy, disorganized, and wet uncolonized looking grains is bacterial.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisibleblindingleaf
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Re: No PC? No worries! Lazy tek [Re: Mad Season]
    #23828066 - 11/13/16 06:32 AM (7 years, 2 months ago)

Quote:

And place your input here so can you help silence the haters




this is ur job as the OP of the write up.

that you tube video seems more likely to succeed


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineKenetic
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Re: No PC? No worries! Lazy tek [Re: Killaloting]
    #23828077 - 11/13/16 06:38 AM (7 years, 2 months ago)

Quote:

Killaloting said:





means that it can be worth for someone to try that maybe never will try if it means investing in to much stuff, thats the whole point that people here doesnt understand, Like i can tell you the best way how to grow stuff and it will cost you 100.000 us to start it or i can suggest another way for small timers that has no serious interest ( at least yet ) so they try and get more interested and therefore perhaps advance





I can understand the logic behind this but if you seriously want to help, try to structure your "tek" a little better because it is border-line incomprehensible.  Just sayin


--------------------
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                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Edited by Kenetic (11/13/16 06:48 AM)


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InvisiblePastywhyteMDiscord
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Re: No PC? No worries! Lazy tek [Re: blindingleaf]
    #23828079 - 11/13/16 06:42 AM (7 years, 2 months ago)

There problem here is that we have a contam prone grain like popcorn, unsterilized and exposed to open air (according to the wording of this tek). If by some miracle that isn't contamed, the fruits from that grow should be cherished.

Eventually these practices will bite you in the ass and it won't be fun. Fruiting the grains as an unbroken colony is going to help keep the bacteria from getting too far ahead but the way you have exposed media eventually mold is going to get a foothold and that will be the end.

No one here is a hater. But we all see that when methods like this one go unchallenged, a few weeks later dozens of threads pop up with people having mold farms on their hands and the OP of the thread in question long gone. If this method works for the OP that is fine. I know that I couldn't make it work and I have grown a mushroom or two.


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InvisibleMad Season
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Re: No PC? No worries! Lazy tek [Re: Pastywhyte]
    #23828084 - 11/13/16 06:44 AM (7 years, 2 months ago)

There's no way I could make it work either. My homes spore load is a fuckin mess :rofl:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineKillaloting
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Re: No PC? No worries! Lazy tek [Re: blindingleaf]
    #23828088 - 11/13/16 06:44 AM (7 years, 2 months ago)

Since everyone is begging for it... im gonna video the whole procedure :smile:

Easier then trying to translate everything anyway, there is alot difference from grain to grain, as air pockets etc as someone mentioned.

This is why I only suggest using popcorn for this method, i have tried cutting corners on other substrates and failed miserly, even WBS mix failed using this method, wheat failed ,oatsmealed failed (this was the worst one hehe 100% fail ratio)

but here it has been working just fine, amazingly good, i mean even if a few jars will contaminate its no biggie, perhaps its some super durable myc, this was a sporeprint from sporebaby here on this site and its a fast colonisator i dont remember its name though.

If you cant understand the point of the post then dont reply cause you will only destroy the interest for thoose who as I said arent willing to invest as much.

And I will prove its working so just wait, current batch i plan to fruit this week or next week. so its almost time to make a new anyway, have a good sunday everyone


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InvisiblePastywhyteMDiscord
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Re: No PC? No worries! Lazy tek [Re: Killaloting]
    #23828090 - 11/13/16 06:46 AM (7 years, 2 months ago)

Popcorn has the worst endospore counts of any grain. Just because it's lower nutrition doesn't mean mold won't grow on it either. It's not a magic mold proof grain. Seriously you have perhaps gotten lucky but I promise that it won't hold up.


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