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Offlinestevtomato
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Registered: 09/12/16
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Yellow spots?
    #23826889 - 11/12/16 06:58 PM (7 years, 3 months ago)

Hello guys,

first post here! And I do have a question. I've made some grain thingy with rye and all nicely colonised. I've cased three jars (and I think most are doing okay, even though some boxes are growing slower than the other...) and I have one jar left. It looks completely white, but when I shine my torchlight on it, there are yellow spots... Now I'm worried that might be a contamination and I didn't see it on the other jars that I cased too... Could you help me please? I've tried looking at the contamination page but couldn't really find it, so...



And here's the cased mycelium with coco coir and vermiculite! The bottom two are the oldest, cased on 5th, then middle three on 6th and the top two are the election babies... :v



Thanks a lot in advance!

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OfflineKenetic
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Re: Yellow spots? [Re: stevtomato]
    #23826927 - 11/12/16 07:10 PM (7 years, 3 months ago)

Uncolonized, wet-looking grain plus the (yellow) metabolites indicate bacteria.  Bacteria seem to be the biggest challenge in this hobby.  You have to prep and sterilize well and use inoculation techniques that result in fast, clean colonization. 

Wild guess (deduction, actually), you inoculated grain with a ms syringe?

Edit:  Election babies, lol


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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Offlinestevtomato
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Registered: 09/12/16
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Re: Yellow spots? [Re: Kenetic]
    #23826941 - 11/12/16 07:15 PM (7 years, 3 months ago)

Hm, well, I used a spore syringe (it's cubensis B+). I sterilised it by PC-ing it for 90 mins and so. But I guess the bacteria came in a bit later as the grains are more or less fully colonised? So, I guess I should just throw this away?

Thanks for the response btw!

Yeah, if those election babies are growing well, at least something real good came out of it xD

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OfflineWeavieWonder
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Re: Yellow spots? [Re: stevtomato]
    #23827005 - 11/12/16 07:31 PM (7 years, 3 months ago)

Looks like metabolites in response to the presence of bacteria.  Most likely the other jars you spawned to bulk were also contaminated.  Your bulk substrate(It's not a casing) looks fucked.  I hate to say it, but I think your project is destined for failure.  Sorry, man.  If you wish to seek further advice...use proper terminology.  Don't refer to your grain spawn or grain jar as a grain thingy.  Also a substrate (the thingy in your tubs) is not called a casing.  That's a substrate.  Most of the members on this site will at the very least ignore you, but more likely verbally barate you for using such terms instead of giving you the advice you need.  Hope this helps.  Good luck sir!

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InvisibleBoogieman47
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Re: Yellow spots? [Re: stevtomato]
    #23827121 - 11/12/16 08:08 PM (7 years, 3 months ago)

Quote:

stevtomato said:
Hm, well, I used a spore syringe (it's cubensis B+). I sterilised it by PC-ing it for 90 mins and so. But I guess the bacteria came in a bit later as the grains are more or less fully colonised? So, I guess I should just throw this away?

Thanks for the response btw!

Yeah, if those election babies are growing well, at least something real good came out of it xD



You can still case the jar and fruit or even make a mono you can get fruits with bacteria its not wise to do so its wasting material if you get mold ... you should look into agar man clean myc, syringe lasts longer, and peace of mind knowing you have clean spawn


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Offlinestevtomato
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Re: Yellow spots? [Re: Boogieman47]
    #23827157 - 11/12/16 08:19 PM (7 years, 3 months ago)

Hey, cheers all for the response! You are all so helpful.

Quote:

WeavieWonder said:
Looks like metabolites in response to the presence of bacteria.  Most likely the other jars you spawned to bulk were also contaminated.  Your bulk substrate(It's not a casing) looks fucked.  I hate to say it, but I think your project is destined for failure.  Sorry, man.  If you wish to seek further advice...use proper terminology.  Don't refer to your grain spawn or grain jar as a grain thingy.  Also a substrate (the thingy in your tubs) is not called a casing.  That's a substrate.  Most of the members on this site will at the very least ignore you, but more likely verbally barate you for using such terms instead of giving you the advice you need.  Hope this helps.  Good luck sir!



I'll make sure to learn my terms better! However, I just realised I inoculated this at the end of September, could that be just because the jar is a bit old?
How does my bulk substrate look fucked, by the way? Those yellow spots are rye, btw, if that's what you mean... Or do you mean something else?

Quote:

noob47 said:
You can still case the jar and fruit or even make a mono you can get fruits with bacteria its not wise to do so its wasting material if you get mold ... you should look into agar man clean myc, syringe lasts longer, and peace of mind knowing you have clean spawn



I think I shall do that, indeed!
About agar, well, I'm still learning and I'll definitely look into that, but atm I've got enough spore syringes to inoculate with. At least that way I can see if it works or if I'm just totally hopeless at it.

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InvisibleBoogieman47
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Re: Yellow spots? [Re: stevtomato]
    #23827180 - 11/12/16 08:26 PM (7 years, 3 months ago)

Well the reason for agar is some syringes can be bad that you can possibly clean up ... so if you use straight spore solution you have a higher chance at contams ... agar is super easy and totally worth the extra work involved


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OfflineKenetic
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Re: Yellow spots? [Re: Boogieman47]
    #23827204 - 11/12/16 08:35 PM (7 years, 3 months ago)

I just had an aa syringe from a sponsor that had three different molds (plus heavy bacteria) in it.  Agar saved my ass from wasting 20 bucks


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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OfflineWeavieWonder
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Re: Yellow spots? [Re: stevtomato]
    #23827574 - 11/12/16 11:07 PM (7 years, 3 months ago)

Quote:

stevtomato said:

I'll make sure to learn my terms better! However, I just realised I inoculated this at the end of September, could that be just because the jar is a bit old?
How does my bulk substrate look fucked, by the way? Those yellow spots are rye, btw, if that's what you mean... Or do you mean something else?




If your grain took that long to colonize, it was probably contaminated, among with many other reasons.  Your bottom two tubs and the tub second from the top seem to have a serious bacterial issue at hand.  Notice the gray discoloration and the mushroom mycelium looks like melted ice cream.  Your grain jars are what contaminated your bulk substrate.  The pic of the bottom of your grain jar is a good example of what a bacterial infection looks like.  Notice the yellow liquid at the 5 o clock position.  Also you see some greasy-looking, uncolonized grains pressed against the jar.  Bacteria can be tricky.  Your grain can fully colonize, but still be contaminated.  This will serve to bite you in the ass later down the road when you G2G, spawn to bulk, or whatever.

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Invisiblemorty422
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Registered: 07/06/16
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Re: Yellow spots? [Re: Kenetic]
    #23827708 - 11/13/16 12:36 AM (7 years, 3 months ago)

Quote:

kenetic said:
I just had an aa syringe from a sponsor that had three different molds (plus heavy bacteria) in it.  Agar saved my ass from wasting 20 bucks




This is why agar is so important - and why I and others always recommend it to new growers.

You have two jars:
-A luck jar
&
-A skill jar

Trusting a spore syringe to be contaminate free is using nothing but the luck jar-and will likely result in diminished yields...and at the most....total failure.

Use the skill jar more and the luck jar less and you'll find yourself with a higher rate of success and bigger yields.

I know the analogy seems silly....but it is one of the most truthful and competent things you can do in this hobby.

Every time you think, "ehh... I hope this works..." - there is always a better way.

MS syringe > agar > clean agar > clean spawn is straight skill.

MS syringe > grain is straight luck.

:cheers:

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OfflinePlazmotech
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Re: Yellow spots? [Re: WeavieWonder]
    #23827808 - 11/13/16 02:17 AM (7 years, 3 months ago)

Quote:

WeavieWonder said:
Looks like metabolites in response to the presence of bacteria.  Most likely the other jars you spawned to bulk were also contaminated.  Your bulk substrate(It's not a casing) looks fucked.  I hate to say it, but I think your project is destined for failure.  Sorry, man.  If you wish to seek further advice...use proper terminology.  Don't refer to your grain spawn or grain jar as a grain thingy.  Also a substrate (the thingy in your tubs) is not called a casing.  That's a substrate.  Most of the members on this site will at the very least ignore you, but more likely verbally barate you for using such terms instead of giving you the advice you need.  Hope this helps.  Good luck sir!




Wow man, I see you around and your advice is excellent, but you don't have to be a dick to this guy. Idk maybe it's just me reading your comment in an aggressive tone in my head though


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow

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Invisiblemorty422
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I'm a teapot

Registered: 07/06/16
Posts: 988
Re: Yellow spots? [Re: Plazmotech]
    #23827840 - 11/13/16 03:03 AM (7 years, 3 months ago)

Quote:

Plazmotech said:
Quote:

WeavieWonder said:
Looks like metabolites in response to the presence of bacteria.  Most likely the other jars you spawned to bulk were also contaminated.  Your bulk substrate(It's not a casing) looks fucked.  I hate to say it, but I think your project is destined for failure.  Sorry, man.  If you wish to seek further advice...use proper terminology.  Don't refer to your grain spawn or grain jar as a grain thingy.  Also a substrate (the thingy in your tubs) is not called a casing.  That's a substrate.  Most of the members on this site will at the very least ignore you, but more likely verbally barate you for using such terms instead of giving you the advice you need.  Hope this helps.  Good luck sir!




Wow man, I see you around and your advice is excellent, but you don't have to be a dick to this guy. Idk maybe it's just me reading your comment in an aggressive tone in my head though




No. This person's comment also came off as being a bit abrasive to me as well...

The advice is usually okay though-so I brush it off.

Hey, WeavieWonder... I know you mean well....but maybe, try not to be so much like bodhisatta..... :lmafo:

:nyan:

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OfflineIAMNOTALLAMA
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Re: Yellow spots? [Re: Kenetic]
    #23827855 - 11/13/16 03:17 AM (7 years, 3 months ago)

Quote:

kenetic said:
I just had an aa syringe from a sponsor that had three different molds (plus heavy bacteria) in it.  Agar saved my ass from wasting 20 bucks




ERG. Me too! I got a AA+ syringe from this vendor. Like half of the jars got contaminated while that didn't happen with any of the other syringes. I contact the vendor and he says he is sorry, and he heard that was happening. So he sends me two more AA+ syringes and I'm happy until a few days ago. Jars from one of the syringes are contaminated and nothing happened with the other one. Damn. :mad2:


--------------------
"It stands as one of the richer ironies of the drug war that the creation of a powerful new taboo against marijuana led directly to the creation of a powerful new plant" - The Botany of Desire


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Offlinestevtomato
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Re: Yellow spots? [Re: IAMNOTALLAMA] * 1
    #23827874 - 11/13/16 03:48 AM (7 years, 3 months ago)

Hey, thanks again guys! Well, I kind of asked for it with all my noobness and failure to use the proper terms. I know how forum works, which is always a mixture of real experienced users and many, many n00bs like me who run around asking for silly questions. So the reaction is rather understandable (it was a good advice too, so).

I do have to clarify that I've left the fully colonised mycelium alone for... quite a few weeks. It actually took merely 3-4 weeks to fully colonised it. I have to say though that the grain might be slightly on the wet side when I inoculated... I did one batch before but the grain must have been too dry, nothing happened. Maybe I have to find a middle ground.

As for the tubs, I think I'll leave them first anyway, so I can see how it turns out later: the more I see, the more I can learn from doing, right?

I see all these advice on taking the extra step to do agar, and I've been reading up on teks after that and I've decided to take your advices and try it out. So I'll try to use more from the skill jar than the luck jar, though I might need to put in more skills into the skill jar before being able to grab anything out of it first... But thanks a lot for the advices :laugh:

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OfflineWeavieWonder
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Re: Yellow spots? [Re: stevtomato]
    #23828232 - 11/13/16 08:21 AM (7 years, 3 months ago)

Perhaps I am a little brash, but at least he learned something for free.  :shrug:  My momma raised me with tough love.  Maybe I'll take a sensitivity class.

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OfflinePlazmotech
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Re: Yellow spots? [Re: WeavieWonder]
    #23830539 - 11/13/16 08:59 PM (7 years, 3 months ago)

Quote:

WeavieWonder said:
Perhaps I am a little brash, but at least he learned something for free.  :shrug:  My momma raised me with tough love.  Maybe I'll take a sensitivity class.




hahaha


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow

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