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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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2nd grow +[pics]
#23826879 - 11/12/16 06:53 PM (7 years, 2 months ago) |
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Well 2nd times the charm, right?
previous attempt failed mistakes were made.. trich  https://www.shroomery.org/forums/showflat.php/Number/23718516
Giving it the beans this time around b+ 1:1 spawn:sub rye grain, coir verm gyp. MS. 1ft2 tub 1 week has passed since initial colonization of sub. surface colonized in less then 2 days, growth was light and fuzzy.
   
The difference in appearance so far from the last grow is slightly alarming. Hopefully you guys will tell me what you think.
Iv also opted to use a white trash bag as the liner this time, for convenience reasons only, However this has allowed me to make out myc growth inside the sub against the bottom and the walls.

Its very possible that there is less spawn distributed on the walls then in the central area.
My Questions thus far: Hows the surface look? Should I wait for the sub to be completely colonized where it meets the liner (walls and bottom of sub) before fruiting?
These pics have been SATURATED so its easier to see the colors. Included is one pic labeled unedited to show what our eyes would see.

Seeing blue, also noticed decreased condensation on the wall so water was added via mister and holes re-stuffed before pics were shot. Bluing has since decreased.
    
Iv circled a few objects of interest, and pointed out something id like taken a look at. Any insight would be most relieving.
   
Early growth
 
   
Close ups
 
Thanks for taking a look! Dr_S
Edited by Dr_Sativa (11/28/16 09:03 PM)
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Re: 2nd grow log +[pics] [Re: Dr_Sativa]
#23827658 - 11/12/16 11:59 PM (7 years, 2 months ago) |
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Fruit it now.
Many people are introducing their tubs to fruiting conditions immediately after spawning to bulk. With great success.
Your sub looks alright.
Time to fruit man!
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: 2nd grow log +[pics] [Re: morty422]
#23827902 - 11/13/16 04:08 AM (7 years, 2 months ago) |
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Quote:
morty422 said: Fruit it now.
Many people are introducing their tubs to fruiting conditions immediately after spawning to bulk. With great success.
Your sub looks alright.
Time to fruit man!

im gonna start a log here soon of a tub fruiting at spawning maybe two
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Re: 2nd grow log +[pics] [Re: spore-ty]
#23829901 - 11/13/16 05:23 PM (7 years, 2 months ago) |
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Thanks guys!
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Timmy Meow



Registered: 04/05/15
Posts: 546
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Re: 2nd grow log +[pics] [Re: Dr_Sativa]
#23830120 - 11/13/16 06:49 PM (7 years, 2 months ago) |
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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These pics have been SATURATED so its easier to see the colors. Included is one pic labeled unedited to show what our eyes would see.

Seeing blue, also noticed decreased condensation on the wall so water was added via mister and holes restuffed before pics were shot. Bluing has since decreased.
    
Iv circled a few objects of interest, and pointed out something id like taken a look at. Any insight would be most relieving.
   
Thanks for taking a look! Dr_S
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Seeing lots of what appear to be Hyphal Knots. 
no obvious sign of contams Misting a lot, read that FAE and evaporation are more important then humidity. If that means misting it 5 or more times a day so be it, or maybe the fan is too powerful.
should a slight breeze be felt near the stuffed holes?
Anyways wondering if its possible the mycelium prioritizes areas with higher air currents to form knots because then the fruits would be in the optimal place to release spores.
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Edited by Dr_Sativa (11/16/16 06:53 AM)
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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knots are becoming more apparent, like little pin heads. Below is a pic of a small section of the tub, circled in blue are knots that are more densely packed together.

Whats a normal time frame for you personally to get from this point to fruits?
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Timmy Meow



Registered: 04/05/15
Posts: 546
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,368
Loc: The Inexpressible...
Last seen: 49 seconds
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Oh shit its gettin good
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
  Oregon Eclipse Festival 2017 :: Aug 19th - 21st :: Pure Paradise   Very Effective LSA Extraction Tek | 💧 Advanced Cold Water LSA Extraction Method 💧 |  Mescajuana - Mescaline with Marijuana | DMT Dab Bongs | UFO Technology! Shpongle
     
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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wow Timtoshis Awesome grow man! a great example and gives me a good estimate.
I sure hope it stays that way LogicaL Chaos!
Since my last post iv reworked the tub once more. Reread Franks guild titled "Dial in your monotubs like a boss" ended up moving the fan further away and made some slight adjustments to the stuffing. Should be ready to rock, and based off of Timtoshis's log id say just in time.
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Timmy Meow



Registered: 04/05/15
Posts: 546
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You're most welcome and thank you.
A very trusted and experienced member made an easy tek to dial in monotubs using micropore tape instead of polyfil. Here's the link if you're interested: https://www.shroomery.org/forums/showflat.php/Number/23651523
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Just might try that next tub. Since the last update more knots have shown up but havent noticed any knot growth yet. Figure its just a matter of time... that is unless a crucial aspect has been overlooked.
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Woot 1st pin formed last night, now all it needs are some friends!
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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An update,
Ohh yea Iv got a good feeling about this one.
More pins showing up, earlier ones are getting bigger, getting excited!
Its not going to be a spectacular yield, but honestly id be satisfied with any sized yield.
Figure the skill will come in time, and now that iv got some of the basics down. 
Il keep you guys posted, figure it must be funny/horrifying watching a noob like me, I just need more pics.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,368
Loc: The Inexpressible...
Last seen: 49 seconds
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personally, i like seeing noobs have sucess at such an early stage
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
  Oregon Eclipse Festival 2017 :: Aug 19th - 21st :: Pure Paradise   Very Effective LSA Extraction Tek | 💧 Advanced Cold Water LSA Extraction Method 💧 |  Mescajuana - Mescaline with Marijuana | DMT Dab Bongs | UFO Technology! Shpongle
     
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egobrain


Registered: 11/17/16
Posts: 180
Loc: Canada
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Dude, I'm stoked for you. My first try I got trich too.
My second tub is almost done colonizing now, hoping for a pinsplosion soon!
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afflictis
ModestMycologist
Registered: 03/09/12
Posts: 334
Last seen: 5 years, 9 months
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Re: Captains log [Re: egobrain]
#23855783 - 11/21/16 09:52 PM (7 years, 2 months ago) |
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Post some pics of the pin set
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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LogicaL Chaos what can I say, this work is whats making the world a better place
egobrain when your pinsplosion comes I wana see it! That goes for afflictis too.
Moneyshot
 Three musketeers
 The one that got away
 Snow white and the 2 dwarfs
Edited by Dr_Sativa (11/21/16 10:59 PM)
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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lookin good . Good job dude.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Thanks JacobStorm!
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Hmmmm... Thinking a white liner was probably not the best idea...
 (upside down)
Are they doomed?? What to do??
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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 mycelium is pretty aggressive
And your gonna get some pins everywhere throughout your substrate no matter what but just pick em when you harvest the whole first flush but im using clear liners now so its no problem and oh ya nice job
Edited by spore-ty (11/25/16 11:51 PM)
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Captains log [Re: spore-ty]
#23867957 - 11/26/16 06:33 AM (7 years, 2 months ago) |
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What cha growin?
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
Dr_Sativa said: Hmmmm... Thinking a white liner was probably not the best idea...
 (upside down)
Are they doomed?? What to do??
Why is a white liner to blame here? If you say light remind me to slap you lol. They pinned there because it was a more preferable area. Which means your surface isn't as ideal. Which means you need to work on your fruiting skills.
Proper surface hydration and FAE is an art that you'll develop (took me a year to figure it out), but never think it's because of a white liner that the surface isn't doing as well. I use no liners and get very little side pins.
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Quote:
ComebackKid said: What cha growin?
Syringe was labeled b+
Quote:
Mad Season said:
Why is a white liner to blame here? If you say light remind me to slap you lol. They pinned there because it was a more preferable area. Which means your surface isn't as ideal. Which means you need to work on your fruiting skills.
Proper surface hydration and FAE is an art that you'll develop (took me a year to figure it out), but never think it's because of a white liner that the surface isn't doing as well. I use no liners and get very little side pins.
Your right Mad Season
Early on when I first placed it in fruiting conditions there was way too much FAE. The surface lost more moisture then I was replenishing.
I still havent found a balancing point yet. Thinking next time a casing layer may give me more flexibility. Currently undecided.
Before the next attempt Ill be modifying the tub with larger diameter holes. And possibly using Pastywhyte's micropore tape tek instead of polyfill as recommended by Timtoshis.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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A casing can help. But all you need is nice little beads of moisture on the sub surface during pinning. Next time mist from far away. Let the mist settle on the sub surface and watch the beads form on top of the sub. Also be careful not to have any pooling water! Youll eventually notice little beads glimmering in the light. That's all you need
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Thanks ComebackKid, Im working in an area that can in no way be considered sterile, so what iv been doing is cracking the lid ever so slightly for misting to avoid air born contams. Is that a valid concern at this stage?
Some close ups
 
 
The first pic shows signs of spores beginning to drop. Should that one be harvested?
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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What kind of unsteril environment are we talkin here? Fully colonized sub is contam resistant and if you're using coir as a bilk substrate even more so. You really shouldn't have any issues with opening your lid to do a proper mist.
Yeah those look about reasy. You could leave em for a few more hours maybe
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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If an ideal environment was a 10 this would be 5 at best. lots of traffic, roommates, dust, clutter, not to mention one of the roommates is a professional bread mold cultivator.
One must make due with whats available
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Honestly, that doesn't sound that bad to me. If mold spores are going to germinate then they will find a way weather you give your shrooms proper conditions or not. 
I would focus more on maximizing your shrooms potential and less about what could go wrong in the process of getting er done 
Also you should give your room mate shit for being a slob
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Quote:
ComebackKid said: Also you should give your room mate shit for being a slob
Every day

After this ill be working a lot with agar, so ill have plenty of time to plan and reflect on the mistakes iv made so far.
List of mistakes this time around: Improper FAE hole size (tools needed) Air currents too powerful (fan too close) Temp control (read cold temps make them small(high 60s and below)) Improper humidification techniques Lacking humidity [Unknown mistake(s) placeholder]
Regarding the agar work Im looking to isolate the strongest genes. So far i have 10 samples from the original MS spawn. In addition I plan on taking samples of each of these fruits, as well as any note worthy ones that come after. Maybe spawn them to trays and see who comes out on top.
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Edited by Dr_Sativa (11/26/16 03:47 PM)
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Picked the ones that were ready and made agar samples from them.
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Status update
Growth is picking up, noticing more pins a few really advanced after those others were picked.
Fruit samples are beginning to spread on the agar. 
Removed the liner to collect side pins, found a relatively large one on the bottom. Took a sample.
Decided to not replace the liner. I think this will help keep the sub better hydrated as the droplets where running down the side of the tub and getting trapped between liner and tub.
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Dr_Sativa
Wonderer



Registered: 10/05/16
Posts: 85
Last seen: 20 days, 12 hours
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Tub was flipped and the bottom had a few "flushes"
 total yield was ~30g bone dry
 some examples pictured
thanks for tuning in peace out!
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