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somedudeonthemoon
Stranger
Registered: 11/11/16
Posts: 4
Last seen: 6 years, 11 months
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Can too much "juice" be injected into my jars from my spore syringe?
#23824592 - 11/12/16 12:10 AM (7 years, 2 months ago) |
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I am very new to this and tried to do a ton of research on the cultivation before hand and ordered pre sterilized jars and some spore syringes, thing is I got a little carried away and when I inoculated the jars I injected probably about 6cc into each jar, maybe more, in 4 spots around the edge of the jar. The jars are only 4oz each and I am worried that there is too much moisture, as there is condensation forming on the inside edges of the jars. Can this excess moisture cause failure of mycelium growth? Is there any way to fix my blunder? Any advice/input would be greatly appreciated. Will post pics if requested. Right now I am keeping them all in a closed metal pot (for darkness) with a thermometer inside and a light near it to keep the temp between 75 and 80 degrees fahrenheit. It has either been 2-3 days or a little over a week since inoculation (my apologies for uncertainness...) and whatever mycelium growth, if any, looks more gooey than hairy and that is really what has me worried.
Thanks in advance for any replies.
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joey
Sunshine Recorder



Registered: 03/04/02
Posts: 135
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: somedudeonthemoon]
#23824616 - 11/12/16 12:21 AM (7 years, 2 months ago) |
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You could have made the jars yourself for probably way less then you paid for "sterilized jars". If you got them in the mail, they were more then likely no longer sterile when you got them.
Also 6cc into each jar is overkill. You want to have around 1cc per jar. With four inoculation points, try to use a single drop in each of them.
Look up RR's pf tek, there's some free videos of it out there. It will show you everything you need to know to get started.
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joey
Sunshine Recorder



Registered: 03/04/02
Posts: 135
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: joey]
#23824619 - 11/12/16 12:23 AM (7 years, 2 months ago) |
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Also, I know it's your first post.
But let it be known, in this community the guys really shy away from any kits or pre sterlized bags/jars that you can buy on line.
The reason is, it's much easier, and cheaper to do it yourself.
Keep reading brother, you'll get the hang of it.
ps- Those jars are probably useless. Get them out of the dark. I don't know what kind of gas exchange they're getting. It's impossible to tell without pictures.
Edited by joey (11/12/16 12:26 AM)
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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: joey]
#23824632 - 11/12/16 12:30 AM (7 years, 2 months ago) |
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They will skin u on here for wasting 6 ml a jar. Most spore syringes you get contain more then 10 times the amount for high amount of active life. If you divide your syringe into 10 more syringes and injected to 100 jars you would still see growth in most of them. No worries first grow mistakes happen
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somedudeonthemoon
Stranger
Registered: 11/11/16
Posts: 4
Last seen: 6 years, 11 months
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: tump]
#23824662 - 11/12/16 12:50 AM (7 years, 2 months ago) |
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I realize now that its already been done the ammount was way too much for each jar, i tried this once before and made the jars myself and got nothing so i just decided to order everything at once to try to better my chances. The jars came in a sealed bag and i wiped the rubber inoculation hole with an alcohol swab before injection, so im not really worried about the sterility of the process, i just wondered if the moisture is gonna completely mess everything up or if i have a chance to fix anything still. Also why should i take them out of the dark? Everything i read said to keep them in a warm dark spot until it was time to transfer to the terrarium. I take the lid off to check temp several times a day and its not an air tight pot, so any co2 or other gas that builds up is definitely not kept inside the pot for long. The jars also have a small hole with some kind of filter tape over it to allow the jars to "breathe." Should i just take some pictures of the jars and my setup and post them?
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joey
Sunshine Recorder



Registered: 03/04/02
Posts: 135
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: somedudeonthemoon]
#23824668 - 11/12/16 12:59 AM (7 years, 2 months ago) |
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Look up RR's video, it will explain a lot of your questions.
Also the jars need light, just like you and I need it.
With no knowledge of how those jars were made, and what you were inoculating into, it's impossible to know what kind of gas exchange they need. Jars need to breath through a filter to keep out contamination. This is usually done trough a dry vermiculite layer, with miropore tape, or filter disks.
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somedudeonthemoon
Stranger
Registered: 11/11/16
Posts: 4
Last seen: 6 years, 11 months
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: joey]
#23824692 - 11/12/16 01:11 AM (7 years, 2 months ago) |
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I will look up video. The jars are made of "high density tempered poly ethyline" and are allegedly filled with "peanut meal nutrient with added minerals" and there is a small hole with what i assume is the tape you speak of on the lid. In regards to the light, can it be from my regular lamp or does it need to be sunlight/simulated sunlight?
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joey
Sunshine Recorder



Registered: 03/04/02
Posts: 135
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: somedudeonthemoon]
#23824699 - 11/12/16 01:16 AM (7 years, 2 months ago) |
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Daylight is best.
or
6500k florescent CFL's
Honestly. I think your best bet is to start over.
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somedudeonthemoon
Stranger
Registered: 11/11/16
Posts: 4
Last seen: 6 years, 11 months
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: joey]
#23824717 - 11/12/16 01:24 AM (7 years, 2 months ago) |
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Okay i appreciate the advice and help. I will probably keep these jars for a while longer and see what happens with them after putting them in the light and fresher air.
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TheShroomster01
It's my first time, dont laugh.



Registered: 10/30/16
Posts: 226
Loc: EU
Last seen: 5 years, 6 months
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: somedudeonthemoon]
#23824839 - 11/12/16 03:11 AM (7 years, 2 months ago) |
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Quote:
somedudeonthemoon said: I will probably keep these jars for a while longer and see what happens with them after putting them in the light and fresher air.
Yeah. keep 'em and see what happens. If anything you'll learn how it shouldn't look.
You will be able to grow them under a CFL but indirect sunlight is preferred.
-------------------- I'm European, i can't help it. "To harvest a cannabis plant to make hash corresponds to slaughtering a cow to make milk. Rub it!"
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Re: Can too much "juice" be injected into my jars from my spore syringe? [Re: TheShroomster01]
#23825087 - 11/12/16 07:26 AM (7 years, 2 months ago) |
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tips on syringe control.
Quote:
blackout said:
Quote:
third3y3 said: It's SOOO easy to squeeze too much. I guess I'll get better. Typical newbie type mistake.
Theres a trick to stop this, do not use the syringe like people typically do. I am right handed, I grasp the syringe in my right hand making a thumbs up sign, the plunger end is sticking up with my thumb under the bit you usually press down with your finger. So if I gave a thumbs up out straight the needle is facing the floor.
My right pinky finger is gripping the body of the syringe at the 10ml sign
 Now you can press down the plunger with your left hand while actively pressing upwards with your right thumb. This gives you good control. If a syringe is unused for a while the rubber seal will be subject to static fritcion (aka stiction). If you use a syringe in a normal manner the moment this stiction is overcome the plunger will tend to move far too much due to the force you are applying and the fact nothing is stopping the syringe from going all the way down.
You can do it with just 1 hand too, just practice with water.
Quote:
blackout said:
I didn't state the obvious, but as the syringe empties obviously my pinky finger moves down the body with the print on it. You can get decent control with just 1 hand too, I usually only use the 2 hand method with a syringe which has not been used in a long time. With the 1 hand method I clench my fist around the syringe. When full my little finger wrapped around the syringe body (with the print) and my other 3 fingers grasp the plunger shaft firmly and my thumb presses down. So the plunger shaft is stopped from moving quickly by the friction of my fingers. As the liquid gets used up I put the next finger on the printed body. For the last 2ml my forefinger is just pressed against the underside of the plunger shaft which again just stops it being able to jump down too much.
Another 1 handed method is having your 3 fingers grasp the body with the printing, and your forefinger wrapped around the plunger shaft which provides resistance. Then press down with your thumb in a controlled manner. As said, just practice with plain water and see what suits you best.
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