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jellyfish


Registered: 10/02/05
Posts: 7,457
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Re: Need some advice [Re: Mikeify]
#24055367 - 01/31/17 06:31 PM (6 years, 11 months ago) |
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I'm using GT as an abbreviation for golden teacher. Since a cube is a cube, I don't see why they would be more prone to bacterial infection than any other variety of cubensis. The print I used to get that rye going was taken by me from my first and only monotub over 3 years ago. I got several flushes out of it and would re-hydrate the bulk sub by taking the tub into the bathroom and filling it up with a bit of water from the bathtub faucet. I wanted to scrap it before it got contaminated, so I did. Had no issues with bacteria.
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Re: Need some advice [Re: jellyfish]
#24118539 - 02/25/17 10:47 AM (6 years, 10 months ago) |
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Don't want to straight a new thread but need advice. I thought I had finally isolated mexicana and golden teacher on afar over successive transfers. One transfer even involved using a stone that formed on a mexicana plate. I inoculated rye, but didn't get a chance to inoculate for a week or so and I'm afraid it may have dried a bit. Also shook all my jars to speed colonization.
I have one jar of mexicana and one jar of GT that seem alright. All my agar cultures I'm going to give up on and start over because they have gotten dry and don't seem too successful. If these 2 jars I post seem okay, I will use them to start a new set of agar plates of mexicana and GT as well as a few spores I got through some trades. My main goal is to grow sclerotia as I have heard mycellium is legal in Canada just not fruiting bodies.
The ones that seem okay: Mexicana:
 GT

There are also 2 other mex jars and 1 other GT jar. They all look like they didn't colonize fully, either I introduced bacteria by shaking, they stalled, or they're something else entirely. It was hard to get a clear pic but one of the mex jars has a thick layer on top that looks more like ice than mycellium of any sort.
Weird mexicana jar1 (with that thick ice looking growth




Weird mexicana jar2:
 messed up this pic. Meant to show how some spots at the bottom are uncolonized and they were inoculated a month ago. Good be the agar got too dry but I feel it's bacterial. I use a flow hood and pressure cook my rye for over an hour so maybe shaking somehow got something through the tyvek lid.
Weird GT jar 1:

Probably going to ditch all but the 2 okay looking jars, use sterilized tools in front of a flow hood to try to inoculate some agar while still letting the mex jar be able to slowly form stores.
Advice very appreciated.
I feel I might need to make this a new post because people are going to see how many replies this thread has and assume my questions have been answered.
Most of my spore collection is over 3 years old. The GT print I took in 2013 actually fruited on agar but obviously one of my 2 GT jars are either stalled or contamed. Should I bother transferring the two decent looking rye cultures back to agar and maybe start some new spores and leave the rest of the mexicana jar to see if it will produce stones. I used jars with holes in the lids like I would normally with cubensis on rye. Should the holes be smaller to encourage CO2 buildup and cause stones to form?
Thanks again.
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